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第 2 期 王宇 等：山羊痘弱毒疫苗 （AV41 株） 接种途径和剂量对牛抗体反应的影响 31

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Effects of inoculation routes and doses of attenuated goat pox vaccine
（AV41 strain） on antibody response in cattle

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WANG Yu ,XIONG Bo ,PENG Qingjie ,XIANG Zhijie ,YUAN Xinwei ,
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WANG Yan ,ZOU Liangxun ,CHEN Yingyu ,GUO Aizhen 1,2
1.College of Veterinary Medicine，Huazhong Agricultural University/
State Key Laboratory of Agricultural Microbiology，Wuhan 430070，China；
2.Hubei Hongshan Laboratory，Wuhan 430070，China； 3.Angel Yeast Co.，Ltd，Yichang 443001，China；
4.Hubei Liangyou Jinniu Animal Husbandry Techology Co.，Ltd，Xiangyang 441034，China
Abstract To evaluate the dynamics of antibodies production after subcutaneous and intradermal inoc⁃
ulation of attenuated goat pox vaccine （AV41 strain） in cattle and optimize the immunization program of
cattle，25 healthy beef cattle were randomly assigned into 5 groups and inoculated subcutaneously and intra⁃
3.5
dermally at 5 and 10 times of goat dose，respectively. The standard single dose was 1.0 × 10 TCID 50
AV41 strain.At 0，15，30，45 and 60 days post vaccination，the serum antibody level was detected by both
virus neutralisation test （VNT） and a commercial ELISA kit.The PCR results of nasopharyngeal swabs
and blood were negative without shedding vaccine strain.The results of VNT and ELISA antibody detec⁃
tion were similar，60% of the cattle in the subcutaneous inoculation with 5 times dose group seroconverted
on day 30 post vaccination，with the maximum neuralising antibody titer of 1∶640，followed by the intrader⁃
mal inoculation with 10 times dose group and subcutaneous inoculation with 10 times dose group （40%），
while only 20% seroconverted in the intradermal inoculation with 5 times dose group.The neutralisation in⁃
dex of VNT was significantly positively correlated with the S/P value of ELISA.The above results suggest
that similar to the inoculation route and dose of relevant international vaccines，subcutaneous inoculation of
AV41 at a 5 times goat dose is safe and effective and can be used as a clinical vaccination protocol for
lumpy skin disease.
Keywords lumpy skin disease； attenuated goat pox vaccine （AV41 strain）； vaccination dose； inocu⁃
lation route； antibody detection； healthy breeding

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