Page 20 - 《华中农业大学学报》2020年第6期

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华 中 农 业 大 学 学 报 第 ３９ 卷

Identificationandex p ressionofTERTinLi p a p hiser y simi Kaltenbach )
(

,
ZHANGXinze , ZHANG Hui j ie , ZHOU Yichen g LIZhao , WANG He y uan , ZHUZhihui
HubeiInsectResourcesUtilizationandSustainablePestMana g ementKe yLaborator y /
Colle g eo fPlantScienceandTechnolo gy Huazhon gA g riculturalUniversit y Wuhan４３００７０ , China
,
,
Abstract TheLi p a p hiser y simi ( Kaltenbach ) isoneofthea g riculturalinsectsendan g erin g crucifＧ
erousve g etables , andithasara p idp ro p a g ations p eedandisdistributedallovertheworld．Telomerase
reversetranscri p tase ( TERT ) isacatal y ticsubunitoftelomerase , whichnotonl y caninducetelomerase
activit y toaffectcella g in ganda p o p tosis , butalsohasotherbiolo g icalfunctionsbesidestelomerasedeＧ
p endent．Inordertoclarif ythemechanism ofthetelomerasereversetranscri p tase ( TERT ) ininsect
g rowthanddevelo p ment , theTERTinL．er y simi ( Kaltenbach ) wasclonedanditss p atialandtem p oral
ex p ressionwasanal y zed．Accordin gtothelocaltranscri p tomelibrar ytherelatedse q uencesofTERT
,
g eneofL．er y simiwerescreened , andtheCDsse q uencesofTERT g enewereclonedb yg eneam p lificaＧ
,
tion．ThefullＧlen g thse q uenceoftheCDSre g ionofthe g eneis２６５８b p whichcanencode８８５aminoacid
residues．Itssecondar ystructurecontains４１al p haＧhelixesand６０coiledcoils．Afteru p loadin g ittothe
NCBI , itwasfoundthatthe g enehasthehi g hesthomolo gywithDiura p hisnoxia , andhaslowhomolo gy
withothers p ecies．Therelativeex p ressionofTERTindifferenta g esandtissuesofL．er y simiwasanaＧ
l y zedb y realＧtimePCR．Theresultsshowedthattherelativeex p ressionofTERT g eneintheadultsta g e
ofL．er y simiwasthehi g hest , andthereweresi g nificantdifferenceswitheacha g e．Theex p ressionlevel
st
of１ instarn y m p hwassi g nificantl y lowerthanthatofadults , butsi g nificantl yhi g herthanthatof２ ,
nd
rd th th
３ and４ instarn y m p hs．Theex p ressionlevelof４ instarn y m p hwasthelowest , buttherewasnosi g Ｇ
nd rd , th
nificantdifferencewiththatof２ and３ instarn y m p hs．Subse q uentl ythe４ instarn y m p hs , whichis
basicall ymatureandhasthelowestex p ressionlevelofTERT g ene , wasselectedfordissection．Wewere
th
abletosimultaneousl ydetecttheex p ressionofTERT g eneinthehead , chestandabdomenof４ instar
n y m p hswiththehi g hestex p ressionintheabdomen．Theseresultssu gg estthattheTERT g ene p la y san
im p ortantroleintheg rowthanddevelo p mentofL．er y simi , andthere g ionwithlowsimilarit ycanbe
selectedasthetar g etre g ionfor g eneinterferenceorsilencesoastocontrolthema j orharmcausedb y L．

er y simiina g riculture．
Ke y words telomerase ; telomerasereversetranscri p tase ; Li p a p hiser y simi ; eneclone ; eneexＧ
g
g
p ression ; biolo g icalcontrol

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