Page 45 - 《华农农业大学学报》2020年第2期

第 ２ 期 谭云飞 等:长江中下游流域 １３ 个克氏原螯虾群体遗传多样性和遗传结构分析 ３ ９

[ １７ ]曹玲亮, 周立志, 张保卫, 等 ． 安徽三大水系入侵物种克氏原螯 nal y sisamon g three g eo g ra p hic p o p ulationsofredswam p cra y Ｇ
虾的种群 遗 传 格 局 [ J ] ． 生 物 多 样 性, ２０１０ , １８ ( ４ ): ３９８Ｇ４０７． fish ( Procambarusclarkii ) inShandon gp rovinceusin gRAPD
CAOLL , ZHOULZ , ZHANGB W , etal．Genetic p atternsof markers [ J ] ．ActaofYan g tzeUniversit y , ２０１４ , １１ ( １７ ): ３７Ｇ４１
aninvasiveProcambarusclarkiip o p ulationinthethreeriver ( inChinese ) ．
[ ２０ ]胡亚成 ． 微卫星标记应用于克氏原螯虾的半同胞家系分析和亲
basinsof Anhuip rovince [ J ] ．Biodiversit yscience , ２０１０ , １８
( ４ ): ３９８Ｇ４０７ ( inChinesewithEn g lishabstract ) ． 子鉴定[ D ] ． 南京: 南京农业大学, ２０１５．HU Y C．Anal y sisonＧ
[ １８ ]曹玲亮 ． 基于微卫星标记的克氏原螯虾遗传多样性研究[ D ] ． 合 halfＧSibfamiliesofProcambarusclarkiiandthep arenta g easＧ
肥: 安徽 大 学, ２０１０．CAO LL．Stud yong eneticdiversit yand si g nmentusin g microsatellite markers [ D ] ．Nan j in g : Nan j in g
p o p ulationstructureofredswam p cra y fish ( ProcambarusclarＧ A g riculturalUniversit y , ２０１５ ( in Chinese with En g lish abＧ
kii ) usin gmicrosatellitemolecularmarkers [ D ] ．Hefei : Anhui stract ) ．
Universit y , ２０１０ ( inChinesewithEn g lishabstract ) ． [ ２１ ]李家乐, 董志国, 李应森, 等 ． 中国外来水生动植物[ M ] ． 上海:
[ １９ ]张龙岗, 杨玲, 刘羽清, 等 ． 山东省克氏原螯虾 ３ 个地理群体遗 上海科学技术出版社, ２００７．LIJL , DONGZG , LIYS , etal．
传差异的 RAPD 分析[ J ] ． 长江大学学报, ２０１４ , １１ ( １７ ): ３７Ｇ４１． Invasives p eciesofanimalsandp lantsinChina [ M ] ．Shan g hai :
ZHANGLG , YANGL , LIU Y Q , etal．GeneticdifferencesaＧ Shan g haiScientific& TechnicalPublishers , ２００７ ( inChinese ) ．

Geneticdiversit y andstructureanal y sisof１３redswam p cra y fish
Procambarusclarkii p o p ulationsinYan g tzeRiverbasin
( )

１ , ２ １ , ２ １ , ２ １ , ２
TAN Yunfei , PENGGuohui , XIONGLi j in g , PENGBo ,
２ ２ １ , ２
WU Yibo , SONGChaowei , BAIXufen g
１．Shuan g shuiShuan g lüInstitute / NationalKe yLaborator yo fCro pGeneticIm p rovement ,
,
Huazhon gA g riculturalUniversit y Wuhan４３００７０ , China ;
,
２．Colle g eo fFisheries , Huazhon gA g riculturalUniversit y Wuhan４３００７０ , China

Abstract Geneticanal y sisof１３redswam pcra y fish ( Procambarusclarkii ) o p ulationsfromdifＧ
p
ferentre g ionsofthe Yan g tzeRiverbasinin China werep erformedusin g８sim p lese q uencere p eats
,
( SSR ) makers．Theallelenumber , herteroz yg osit y p ol y mor p hisminformationcontent ( PIC ), Hard y Ｇ
Weinber ge q uilibriumindexandg eneticdistanceinthe１３p o p ulationswereidentified , res p ectivel y ．The
resultsshowedthattherewere３Ｇ５allelesateachSSR markerlocusinthep o p ulation , theex p ectedhetＧ
eroz yg osit ywere０．３７Ｇ０．５７ , theobservedheteroz yg osit ywere０．２３Ｇ０．４８ , andthePICwere０．４０Ｇ０．５６ , reＧ
,
s p ectivel ysu gg estin g ahi g her g eneticdiversit y amon g theinvesti g ated p o p ulations．Inaddition , ３g rou p s
wereclassifiedb y anei g hborＧ j oinin gmethodbasedonthe g eneticdistanceamon g the p o p ulations．Taihu
,
(
( TH ) andPo y an g hu ( PYH ) o p ulationsre p resentedthefirstg rou p Chon gq in g CQ ) and Chaohu
p
( CH ) o p ulationsre p resentedthesecondg rou p andHon g hu ( HH ), andDon g tin g hu ( DTH ) andHon g Ｇ
p
zehu ( HZH ) o p ulationsre p resentedthethirdg rou p ．TheseresultsindicatethatChinahasaredswam p
p
cra y fishresourceofhi g hg eneticdiversit y ．
Ke y words redswam p cra y fish ( Procambarusclarkii ); sim p lese q uencere p eats ( SSR ) marker ; eＧ
g
;
p
neticdiversit y g eneticstructure ; o p ulationdifferentiation
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