Page 41 - 《华中农业大学学报（自然科学版）》2022年第4期

第 4 期 陈铭泽 等：微波辅助硫酸水解肉骨粉的工艺条件优化 35

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Optimizing process conditions for sulfuric acid hydrolysis of meat and
bone meal with microwave assistance

1
1,2
1
1,2
CHEN Mingze ,WANG Yanzhen ,FENG Chen ,CAI Ao ,
1
1,2
CAO Yunyun ,ZHOU Wenbing ,XIAO Naidong 1,2
1.College of Resources and Environment，Huazhong Agricultural University，Wuhan 430070，China；
2.The Cooperative Innovation Center for Sustainable Pig Production Co-Sponsored by
Province and Ministry，Wuhan 430070，China
Abstract The meat and bone meal obtained from the harmless high-temperature fermentation of sick

and dead livestock and poultry was used to develop and utilize the resources of sick and dead animal carcass‐
es. After the oil was removed by the extractant，the amino acid-containing hydrolyzed liquid was obtained
by sulfuric acid hydrolysis without or with the assistance of microwave，and the conditions for hydrolysis
were optimized. The material balance of macro，medium and micro plant nutrients and heavy metal ele‐
ments in the materials before and after hydrolysis and their distribution in the hydrolysis products under opti‐
mal conditions were analyzed. The results showed that the opitmal extractant was n-hexane，with the oil ex‐
traction rate of 100% and the recovery of extraction agent of 97.06% at the ratio of solid to liquid 1∶10.
The optimal sulfuric acid hydrolysis conditions for degreased meat and bone meal without microwave assis‐
tance（conventional）were as follows：sulfuric acid concentration of 5 mol/L，solid-liquid ratio of 1∶4，
temperature of 90 ℃，hydrolysis time of 7 h under which the conversion rates of total nitrogen and amino
acid-nitrogen in hydrolysate were 93.42% and 42.63%，respectively. The optimal sulfuric acid hydrolysis
conditions for degreased meat and bone meal with microwave assistance were as follows：microwave power
550 W，sulfuric acid concentration of 5.0 mol/L，hydrolysis time of 60 min. Under these conditions，the
conversion rates of total nitrogen and amino acid-nitrogen in hydrolysate were 90.12% and 82.13%，respec‐
tively. Under the optimal conditions of the two processes，there was little difference in the distribution of
each element in the liquid phase of hydrolysate and the solid phase of residue. Among them，66%-93% of
elements of N，P，K，Fe，Cu and Zn in the feedstock were distributed in the hydrolysate，more than 32%
of the elements of Ca，Mg and Mn were distributed in the residue，and more than 58% of the heavy metal
elements，Pb，Cr and Cd，entered into the hydrolysate，but did not exceed the limit of agricultural indus‐
try standard of related products. It is indicated that microwave assisted hydrolysis significantly increased the
conversion rate of amino acids and decreased the hydrolysis time. Most of the medium and micro nutrient el‐
ements entered the liquid phase，which had obvious advantages in the preparation of amino acid hydroly‐
sate. It will provide basis for the subsequent preparation of amino acid liquid fertilizer.
Keywords sick and dead animal；meat and bone meal；hydrolysis；microwave assistance；material
balance；amino acid liquid fertilizer

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