Page 33 - 《华中农业大学学报（自然科学版）》2022年第4期

第 4 期 张晟国 等：堆肥发酵功能菌株的筛选及其在鸡粪好氧堆肥中的应用 27

Screening of functional strains for composting fermentation and its
application in aerobic composting of chicken manure

ZHANG Shengguo,PENG Yan,LI Ruyu,LI Zhanxin,HUANG Yihao,
WANG Guolei,REN Zhuqing,WU Jian
College of Animal Science and Technology，Huazhong Agricultural University，Wuhan 430070，China

Abstract Livestock manure causes serious environmental pollution problems，and aerobic compost‐

ing can realize the harmless treatment of livestock manure. However，traditional aerobic composting still
has problems such as slow temperature rise，long cycle，serious nutrient loss and serious odor volatiliza‐
tion，and the efficiency and quality of composting need to be further improved. The addition of external mi‐
crobial inoculants can improve the traditional natural composting process of livestock manure. The function‐
al strains for composting fermentation were screened by analyzing the enzyme production and deodorization
ability of the strains，and two kinds of compound microbial inoculants were obtained to develop the microbi‐
al inoculants with high efficiency of degradation，transformation，deodorization and nitrogen-fixation for aer‐
obic composting of livestock and poultry manure，and to further improve the efficiency and quality of com‐
posting. The effects of adding compound microbial inoculants on the conventional physical and chemical in‐
dexes，the activity of cellulase and urease，the loss of nitrogen and the product quality of aerobic compost‐
ing of chicken manure were studied. The results showed that the compound microbial inoculant significantly
increased the cellulase activity of the composting due to its higher ability to degrade organic matter，and the
activity of cellulase reached 4.937 U/g at the later stage compared with the control and the compound mi‐
crobial inoculant Ⅲ. Adding compound microbial inoculant Ⅰ significantly increased the temperature of the
composting，and the maximum temperature reached 65.8 ℃，which was 5.8 ℃ higher than that of the con‐
trol. The compound microbial inoculant Ⅱ significantly reduced the activity of urease and the loss of nitro‐
gen of the composting because it contained the bacteria with strong ability of deodorization，and the loss of
nitrogen was lower than that of the control. The content of total nutrient and the quality of compost prod‐
ucts were improved by adding compound microbial inoculant Ⅱ，and the total nutrient of compost products
was 5.32%，which was higher than that of the control（4.52%）. The addition of compound microbial inoc‐
ulant Ⅰ and Ⅱ both accelerated the maturity of compost. On the 7th day of composting，the GI of seed
germination index of adding compound microbial inoculant Ⅰ and Ⅱ was 75.12% and 75.29%，while the
GI of the control was only 47.89%. It is indicated that the compound microbial inoculants developed has a
good effect in the application of aerobic composting of chicken manure，which can increase the temperature
of composting and the activity of cellulase，reduce the activity of urease and the loss rate of total nutrient
and nitrogen of compost products.
Keywords chicken manure；aerobic composting；compound microbial inoculant；cellulase；urease；

deodorization；organic fertilizer production

（责任编辑：赵琳琳）