Page 42 - 《华中农业大学学报》2020年第6期
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华 中 农 业 大 学 学 报 第 ３９ 卷
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２０００ , ３９ ( ８ ): １Ｇ３．LIUCL , LIJD．RecentadvancesonthedeＧ [ J ] ．Pesticides , ２０００ , ３９ ( ８ ): １Ｇ３ ( inChinesewithEn g lishabＧ
velo p mentoffun g icidesusedforcontrolofoom y cetediseases stract ) ．
Anal y zin gp h y sicochemical p ro p ertiesandcharacteristics
ofXanthomonascam p estrissecretar yp rotein
,
QIN Yue , ZHU You p en g HANChan g zhi
YunnanProvinceKe yLaborator yo fForestDisasterWarnin gandControl ,
,
Colle g eo fBiodiversit yConservation , SouthwestForestr yUniversit y Kunmin g６５０２２４ , China
Abstract Xanthomonascam p estrisbelon g toGramＧne g ativebacteria , whichmainl ydama g ecrucifＧ
erousve g etablesandeconomicforestsincludin gwalnutsandman g osteens , causin gg reateconomiclosses
toa g riculturalandforestr ycro p sandseriousl yharmin g thehealth ydevelo p mentofthea g riculturaland
forestr y industr y ．Secretedp roteins p la yanim p ortantroleinthep atho g enic p rocessof p lant p atho g enic
fun g i , bacteria , andoom y cetes．Thereisnore p ortsonthep h y sicochemical p ro p ertiesandcharacteristics
ofsecretar yp roteinsofXanthomonas．Thisstud yusesthesecretar yp roteinse q uencesofX．cam p estris
B１００ , X．cam p estrisp v．cam p estrisstr．８００４ , andX．cam p estrisCN１４fromthe p ublishedg enomeＧwide
se q uenceasthebasicdata , andusesProtscale , SMART , Tar g etP２．０Server , andotherbioinformatics
softwaretoanal y zethe p h y sicochemical p ro p erties , conserveddomains , andtransit p e p tidesofthesecreＧ
tar yp roteinsaboveＧmentioned．TheresultsshowedthattherewasnoobviousrulebetweenthetheoretiＧ
calisoelectricp ointofsecretar yp roteinsandthelen g thofaminoacidsinX．cam p estris．Anavera g e４４．
７３％ ofthep roteinisunstablep rotein．Anavera g eof８３．２１％ ofthep roteinhasatotalavera g eh y droＧ
p hilicit yoflessthan０ , whichisah y dro p hilicp rotein．Thenumberofp roteinswithobviousconserved
domainsineachsecretar yp roteinofXanthomonasis１２onavera g e．Allsecretar yp roteinshavesi g nal
p e p tides．Thedistributionoftransit p e p tidep redictionp ossibilitiesisrelativel yeven．
Ke y words Xanthomonas ; secretar yp rotein ; h y sicochemicalp ro p erties ; bioinformatics ; athoＧ
p
p
g en ; subcellularlocalization ; transit p e p tide
( 责任编辑: 张志钰)
