Abstract:
[Objective] To explore and utilize the benefit microbes and their functions for developing sustainable agriculture, the effects of different associated crop on endophytic microbial community structure in root of tomatoes were analyzed. [Methods] Five associated crops and monoculture treatments, i.e., tomato×lettuce(A)、tomato×amaranth(B)、tomato×cabbage(C)、tomato×chive(D)、tomato×mint(E) and monoculture(F) were set under the identical conditions in this experiment. And the endophytic microbial community structures in roots of tomatoes were analyzed using MiSeq high-throughput sequencing technology. [Result] Allorhizobium, Neorhizobium, Pararhizobium, Burkholderia, Caballeronia, Paraburkholderia, Devosia, Nocardioides and Streptomyces were the common dominant endophytic bacterial genera in roots of tomatoes under associated and monoculture systems. However, norank_f_Roseiflexaceae and Sphingobium were the special dominant endophytic bacterial genera in roots of tomatoes under tomato x lettuce (A) associated system; Mea nwhile, Amycolatopsis and Herpetosiphon, were also the unique dominant endophytic bacterial genera in roots of tomatoes under tomato x chive (D) associated system. Moreover, Bradyrhizobium and Dyella, were the special dominant endophytic bacterial genera in roots of tomatoes under tomato x mint (E) associated system; By contrast, Flavobacterium was the unique dominant endophytic bacterial genera in roots of tomatoes in monoculture system.
In addition, unclassified_p_Ascomycota was the only common dominant endophytic fungal genera in roots of tomatoes under associated and monoculture systems. And lmmersiella was the special dominant endophytic fungal genera in roots of tomatoes under tomato x lettuce (A) associated system; Meanwhile, Arachniotus, Neocosmospora, Penicillium, Phialemonium, and unclassified_c_Agaricomycetes were the unique dominant endophytic fungal genera in roots of tomatoes under tomato x amaranth (B); Geminibasidium, Plectosphaerella and Rhizophagus were the unique dominant endophytic fungal genera in roots of tomatoes under tomato x cabbage (C); Acrocalymma_vagum, Arachniotus_dankaliensis, unclassified_o_Boletales, unclassified_p_Basidiomycota, and unclassified_p_Chytridiomycota were the special dominant endophytic fungal genera in roots of tomatoes under tomato x chive (D); Chaetomium_cochliodes, Neocosmospora_rubicola, Penicillium_simplicissimum were the special dominant endophytic fungal genera in roots of tomatoes under tomato x mint (E); By contrast, Gibberella_intricans was the unique dominant endophytic fungal genera in roots of tomatoes under monoculture system (F). [Conclusion] In comparison with the monoculture of tomato, the compositions of dominant endophytic bacteria and fungi at phylum or genera level in roots of tomatoes all could be altered by associating cultivation with other crops. Meanwhile, the special dominant endophytic bacterial of fungal genera all could be enriched by associating with other crops. In suggested that the anti-biotic or abiotic stresses abilities of tomatoes could be improved by associating with the other 5 different crops in this paper.