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  • 1  Study on the Adjust Blood Lipid by Procyanidin of Lotus Leaf Tea
    孙智达 杨尔宁 李斌
    2004, 23(6).
    [Abstract](3406) [HTML](0) [PDF 0.00 Byte](152)
    2  Combining Ability Analysis in Rice Using Recombinant Inbred Lines 
    FU Xin-min WANG Yan GAO Guan-jun HE Yu-qing
    2010, 29(4):397-402. DOI: 1000-2421(2010)04-0397-06
    [Abstract](3353) [HTML](0) [PDF 980.48 K](2887)
    Seventeen restorer lines with fertility restorer (Rf3/Rf4) and plant hopper resistance (Bph14/Bph15) from B5 genes were obtained with molecular marker-aided selection from recombinant inbred lines constructed through crossing Minghui 63 with B5 and Zhongguoxiangdao.Combining ability,heritabilities and correlation of nine agronomic traits were analyzed using NCII experiment design to cross the 17 restorer lines with 3 wild-abortive type cyto-plasmic male sterile(CMS-WA)lines.The results showed that the general combining ability of nine agronomic traits except tillers per plant were significantly different (P<0.05 and P<0.01).The special combining ability of heading date,grains per panicle and seed setting were highly different(P<0.01).These traits were controlled largely by additive effect.Most traits were affected by sterile lines except plant height and seed setting influenced much more by restorer lines.The broad heritability and narrow heritability of most traits were higher than 60%.Broad heritability and narrow heritability of tillers per plant and yield were lower than 50%.Yield per plant were significantly correlated with tillers per plant,grains per panicle and seed setting at P<0.05and P<0.01.Correlation coefficient were significant at P<0.05 andP<0.01 between general combining ability and phenotypic values for most traits.
    3  Advances in Research Potassium Nutrient and Appliment of Cotton
    xiaying 姜存仓
    2009, 28(1):1-5.
    [Abstract](3263) [HTML](0) [PDF 0.00 Byte](2191)
    Advances in Research Potassium Nutrient and Appliment of Cotton XIA Ying1) JIANG Cun-cang1) CHEN Fang2) LU Jian-wei1) LI Xiao-kun1) Hao Yan-shu1) ( 1) College of Resources and Environmental Sciences , Huazhong Agricultural University , Wuhan 430070 , China; 2) Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China) Abstract The article reviewed the effect of potassium fertilizer on yield and the chang trends, technique of applying fertilizer in main cotton production regions in China in recent 50 years, elaborated the effect of potassium on the nutrient function, resistance and quality.The effect of potassium fertilizer was difference in different periods and regions.In recent years, as the promotion of potassium fertilizer and increase the applied amount, the effect of potassium on increasing yield in cotton was decrease gradually in some cotton production regions. So, the necessity of potassium fertilizer, applied time and applied amount should depend on the potassium condition in soil and the rule of requirement of fertilization.
    4  Virus-Induced Gene Silencing-Mediated Viral Resistance of Transgenic Papaya to Papaya ringspot virus (PRSV)
    阮小蕾 王加峰 李华平
    2009, 28(4).
    [Abstract](3175) [HTML](0) [PDF 0.00 Byte](381)
    将PCR检测呈阳性的T4代转复制酶(replicase,Rep)基因番木瓜植株在苗期接种番木瓜环斑病毒(Papaya ringspot virus,PRSV)Ys株系,定期采取不同部位的叶片进行Northern blot分析.结果表明:接种PRSV之前,在植株的各部位均能检测到转基因完整的Rep mRNA,但接种后不同时间在接种叶以上部位陆续出现了Rep mRNA的降解;接种后30 d内,接种叶下部第1片叶上始终未出现Rep mRNA的降解;另外,在发生mRNA降解的叶片上都能相继检测到小分子干涉RNA(short interferring RNA,si RNA)的产生.这说明转基因番木瓜的抗病性与mRNA的降解及siRNA的积累有着密切的关系.这种抗性发生在转录后水平上,是由病毒诱导的基因沉默(virus-induced gene silencing,VIGS)介导产生的.
    5  Relationship between Leaf Anatomical Structure and Heat Resistance of Primula
    HU Wei-juan ZHANG Qi-xiang PAN Hui-tang DONG Ling-ling
    2010, 29(3):363-368. DOI: 1000-2421(2010)03-0363-06
    [Abstract](2601) [HTML](0) [PDF 11.41 M](3977)
    The index of heat injury and leaf anatomical structure under the scanning electron microscope of six Primula species were measured to study the responsive mechanism of Primula to the high temperature and to select the heatresistant Primula species.The results showed that Primula forrestii and P.malacoides franch had higher heat-resistance than that of P.obconica,P.veris,P.saxatilis,P.denticulatess.,P.sinodenticulata with lowest heat-resistance.Leaf anatomical structures of Primula associated with heat-resistance included leaf thickness,tightness of mesophyll cell arrangement,the number of open stomata,the number of epidermal hair and powders of leaves.
    6  Effects of nitrogen deficiency on nitrogen metabolism and expression of genes related during vegetative growth stage of rice
    罗 凤 卢永恩 杨 猛 练兴明
    2012, 31(1):16-22. DOI: 1000-2421(2012)01-0016-07
    [Abstract](2440) [HTML](0) [PDF 1.07 M](5138)
    Nitrogen is one of essential nutrients for plant growth.Under the N deficiency at different time points,the dynamic changes of the expression of genes and activity of enzymes related to nitrogen assimilation in rice were investigated.The results showed that the concentrations of ammonium and nitrate significantly reduced under the N deficiency.Under the N deficiency for a short time,the expression level of genes including NR1, NR2, NiR2,GS2,Fd-GOGAT,GDH2,GDH3 in the shoots as well as NR1,NR2,GDH4 in the roots were up-regulated,and then their expression level decreased after a long time under the N deficiency.Along with the extension of time under the N deficiency,the GS,Fd-GOGAT activities in whole plants,NR activity in shoots and NADH-GOGAT activity in roots decreased while the NADPH-GDH activity in shoots increased.In roots the activities of NR,GDH increased at first and then decreased,while in shoots the NiR activity was just the opposite.Re-supplied N after 7 days of N deficiency,the transcriptional expression level and enzyme activities of NR,NiR,GS,GOGAT,GDH restored,and expression level of some genes increased as well.
    Li Xueyuan
    [Abstract](2294) [HTML](0) [PDF 0.00 Byte](169)
    The clay mineral and chemical composition of clay fraction, morphologic al feactures, physical and chemical properties, as well as cation exchangeproperties of three soil profiles collected from Wuhan were studied. The soil formingconditions and genesis p
    8  Dynamic Analysis on Quality of Salted Eggs in Pickling Process
    RONG Jian-hua ZHANG Zheng-mao HAN Xiao XIONG Shan-bai
    2006, 25(6).
    [Abstract](2253) [HTML](0) [PDF 0.00 Byte](111)
    The effects of pickling temperature,time and concentration of salt solution on maturation of salted eggs were investigated.The salt content and yolk index increased obviously with the increase of pickling temperature,time and concentration of salt solutio
    9  Design of mountain orchard detachable unidirectional traction double track cargo vehicle
    欧阳玉平 洪添胜 焦富江 苏建 徐宁 李震 陈金德
    2015, 34(1):128-135.
    [Abstract](2249) [HTML](0) [PDF 1.21 M](5167)
    In order to overcome the transition,field operation, and standardized batch production difficulties of the existing track cargo vehicle in mountainous orchard, as well as to raise the utilization rate, the mountain orchard detachable unidirectional traction double track cargo vehicle was developed. This paper introduced the whole machine structure, the wire rope diameter selection,the minimum track inclination,the maximum track distance,the minimum track turning radius and the working principle of the rope brake device. Load experiments were carried out to test the cargo′s synthetic track function. It is indicated by calculation that the wire rope diameter should be 7.7 mm,the minimum track inclination was 5.7°, the maximum track distance was 170 m, the minimum track horizontal turning radius was 7 m, and the minimum track vertical turning radius was 2 m. Load experiment results indicated that the cargo vehicle′s uplink average speed was 0.51 m/s, the downlink average speed was 0.54 m/s, the average energy consumption was 1.235 kW·h and the effective use degree was 100%. This cargo vehicle was suitable for fruit, pesticide, chemical fertilizer and other cargo transportation in the mountain orchard. Meanwhile, it can be applied to carry the spraying machine, the shave machine and other agricultural machines.
    10,a Perl script for searching motifs in a group of related DNA/protein sequences
    李旭凯 彭良才 王令强
    2014, 33(04):1-6.
    [Abstract](2198) [HTML](0) [PDF 1.17 M](2454)
    A motif is a sequence pattern occurring repeatedly in a group of related DNA or protein sequences,and is an important concept for describing the common structure and function shared by the members of a protein family.However,the motif can be quite complex and is often difficult to predict the pattern of amino acid sequence.To get the desired results of the short motifs (2-4 polypeptides) derived from various bioinformatics is still a difficult task.The can be used to solve this problem and provide a convenient set of Perl script for working with biological sequence motif.A Perl script was written for searching very similar amino acid sequence pattern or motif in a group of related protein sequences by matching all the possible amino acids fragments permutation and counting frequency and position of each motif matched in sequence.
    11  Effects of germination conditions on growth characteristics and nutrition of mung bean sprout
    尹涛 丁俊胄 陈芸 谢雯雯 熊善柏 赵思明
    2015, 34(4):120-124.
    [Abstract](2121) [HTML](0) [PDF 976.50 K](7980)
    Effects of soaking temperature,cultivation temperature and watering frequency on the growth characteristics and nutrition of mung bean sprout were investigated. Growth characteristics and nutrition of mung bean sprout were significantly affected by soaking temperature,cultivation temperature and watering frequency (P<0.05). When the bean was soaked at higher temperature (40℃),body weight of the cultivated mung bean sprout was heavier,hypocotyl was longer,contents of free amino acid (FAA),water soluble sugar (WSS) and γ-aminobutyric acid (GABA) were higher. The hypocotyl was longer while the diameter was smaller when the bean was cultivated in the relatively higher temperature (30-35℃). When the watering frequency decreased,weight,hypocotyl length and diameter decreased,WSS content decreased first and then increased,FAA content increased first and then decreased,GABA content was fluctuated. The optimal germination conditions of mung bean sprout were soaking bean at 40℃ for 5 h,with cultivation temperature of 25-30℃ and watering once per hour.
    12  Comprehensive degree of land-use at different scales based on spatial autocorrelation regression and geographically weighted regression models
    宁秀红 郭龙 张海涛
    2013, 32(4):48-54. DOI: 1000-2421(2013)04-0048-07
    [Abstract](1959) [HTML](0) [PDF 1.58 M](6647)
    The spatial distribution of Valley County land use degree in 2009 was studied and the spatial autocorrelation of land use degree and its driving factors in different scales were analyzed.A spatial autoregressive model and geographically weighted regression model was established from the perspective of global local.The results showed that there was a certain spatial autocorrelation in land use degree and its driving factors with scale effect.Spatial regression model could estimate the overall parameter of various factors while geographically weighted regression model could give the local parameter of various factors.Scales of geographical weighted regression model could reflect the detailed geographical information to some extent.
    13  Nitrogen Nutrition Characteristics of Rice T-DNA Insertion Mutant
    刘辉 赵竹青 叶志娟 杨野
    2009, 28(4).
    [Abstract](1846) [HTML](0) [PDF 0.00 Byte](381)
    14  Effect of bio-floating bed on growth performance and flesh quality of fish in culture pond
    王景伟 李大鹏 潘宙 郭威 谢从新 何绪刚 亓成龙
    2015, 34(4):108-113.
    [Abstract](1813) [HTML](0) [PDF 1.06 M](9673)
    In this study,we investigated the effect of bio-floating bed on growth and muscle quality of grass carp (Ctenopharyngodon idellus) and blunt snout bream(Megalobrama amblycephala) in ponds.Two modes of culture ponds were chosen:one equipped with bio-floating bed was the experimental pond,and the other without beds was the control pond.At the end of the experiment,we measured the growth parameters,the muscle conventional nutrients,water holding capacity (WHC),and texture characteristics using biochemical and physical methods.The results showed that the final weight of fish cultured in the experimental pond was higher than that in the control pond.The hepatic somatic index of grass carp (1.77%) in the experimental pond was significantly lower than that of the control (2.47%).The condition factors of grass carp and blunt snout bream (1.73 and 2.56,respectively) were significantly higher than those of the control (1.64 and 2.40,respectively).There was no significant difference in the eviscerated weight of both carp and bream between the experimental (91.06 and 88.28,respectively) and the control (89.22 and 88.49,respectively) ponds.The drop loss (14.49% and 10.69,respectively) and water loss rate (17.52% and 10.34%,respectively) of the experimental grass carp and blunt snout bream were significantly lower than those of the control.The cooked rate of the experimental fish was significantly higher than the control.The muscle crude fat content (1.89%) and crude protein content (14.60%) of grass carp in the experimental ponds were significantly higher than those of the control (1.54% and 13.46%),whereas muscle water content (81.68%) of the experimental fish was significantly lower than that (82.65%) of the control.There is no significant difference in muscle ash content between the two groups.Unlike grass carp,only muscle crude protein content (17.23%) of the experimental bream was significantly higher than that (15.90%) of the control.The muscular hardness,gumminess,and chewiness of the experimental carp (5 298.10 g,2 450.78 g and 770.78 g,respectively) were significantly higher than those of the control (3 226.79 g,1 881.43 g and 602.80 g,respectively).The resilience (0.31) and cohesion (0.48) of experimental carp were significantly lower than those (0.43 and 0.58,respectively) of the control.However,only the muscular springiness of the blunt snout bream in the experimental pond was significantly higher than that in the control pond.
    15  De novo assembly of teosinte seedling transcriptome defined by RNA-Seq
    肖之夏 郑用琏
    2014, 33(2):15-21. DOI: 1000-2421(2014)02-0015-07
    [Abstract](1805) [HTML](0) [PDF 980.62 K](4104)
    Teosinte is the ancestor of maize,and plays an important role in maize domestication process and gene cloning.Solexa RNA-Seq was used to de novo assembly and analyze the transcriptome of teosintes.40.6 GB raw data were produced,including 175 101 250 reads of 76 bp length.After quality control and de novo assembly,58 147 teosinte transcripts with an average length of 1 335 bp were obtained.After bioinformatically comparing,it was found that 94.3% of teosinte transcripts had good matching with B73 cDNAs,and that 84.1% of the transcript had good matching with rice,84.6% with sorghum and 83.9% with brachypodium at protein level.This research will provide a reference for subsequent studies on maize evolution and gene discovery.
    16  Isolation and characterization of a novel rice constitutive promoter
    魏 晶 毛伟华 林拥军 陈 浩
    2012, 31(2):139-146. DOI: 1000-2421(2012)02-0139-08
    [Abstract](1757) [HTML](0) [PDF 2.72 M](3282)
    A constitutive gene (TIGR Locus:Loc_ Os07g34589) was obtained by combining our lab’s database with RT-PCR verification.A novel rice constitutive promoter named PSUI1 with 1 941 bp was isolated using the genomic DNA of the indica rice variety Minghui63 based on a PCR method.To determine the expression strength and pattern of the novel constitutive promoter,the cloned promoter was fused with the β-glucuronidase (GUS) reporter gene to form plant expression vector DX2181b-PSUI1,and the maize ubiquitin promoter was also fused with GUS reporter gene to form expression vector DX2181b-PUbi as the control.Both DX2181b-PSUI1 and DX2181b-PUbi were transformed into japonica rice variety Zhonghua11 by Agrobaeterium-mediated method.GUS staining showed that GUS gene was expessed in different rice tissues and organs at different growth stages of DX2181b-PSUI1 transgenic rice plants.Quantitative analysis of GUS activity showed that the strength of maize ubiquitin promoter was 2-3 times higher than that of the PSUI1 promoter,but the expression of PSUI1 promoter exhibited much more stable than that of maize Ubiquitin promoter between transgenic rice lines.
    17  Analyzing genetic diversity of Quercus aquifolioides in different altitudesof Jade Dragon Snow Mountain in the northwest of Yunnan Province with AFLPs
    程小毛 李响 姜永雷 黄晓霞
    2017, 36(1):22-27.
    [Abstract](1735) [HTML](0) [PDF 960.35 K](1780)
    The genetic diversity of Quercus aquifolioides population located at different altitudes (2 750~3 500 m) of Jade Dragon Snow Mountain in the northwest of Yunnan Province was investigated with amplification fragment length polymorphisms (AFLPs) markers.The results showed that the ratio of polymorphic loci was 100%.Shannon diversity index I was 0.54 and the Nei’s gene diversity index He was 0.37,indicating that the Quercus aquifolioides had high genetic diversity at species level.I and He wereagreed,indicating that the genetic diversity of 6 populations varied with altitude gradient.The genetic diversity from 3 050 m to 3 350 m was high.The genetic diversity of 2 7502 900 m and 3 500 m group was decreased.Results of the genetic differentiation of Quercus aquifolioides Gst (0.089) and AMOVA analysis (Phist = 0.110) showed that there was a low level of genetic differentiation among populations.Gene flow among population was 5.12,suggesting that high gene flow may be a main factor leading to high levels of the genetic diversity among populations.
    18  Analysis on the Current Status of Utilization of Crop Straw in China
    Gao Xiangzhao ) ) Ma Wenqi ) Ma Changbao ) Zhang Fusuo ) Wang Yunhua )
    2002, 21(3).
    [Abstract](1726) [HTML](0) [PDF 0.00 Byte](174)
    根据全国农业技术推广服务中心的 2 0 0 0年度土壤肥料专业统计数据 ,分析研究了中国秸秆资源数量、利用途径及养分去向。结果表明 ,2 0 0 0年全国秸秆资源总量达 5 .5 4 1× 10 8t,其中含有的N、P2 O5、K2 O分别为4 .939× 10 6t、15 6 7× 10 6t、9.82 5× 10 6t,总养分为 1.6 33× 10 7t;在作物秸秆资源中 ,以水稻、玉米和小麦为主 ,共占76 .1% ;秸秆利用中 ,肥料、燃料和饲料量分别占秸秆资源的 36 .6 %、2 3.7%和 2 2 .6 % ,其它如原料、焚烧和弃置乱堆共占 17% ;秸秆资源中的养分利用也是以肥料量最多 ,其次是燃料和饲料 ,原料、焚烧和弃置乱堆较少 ,N、P2O5和K2 O养分还田率分别为 4 7.3%、82 .9%和 84 .4 % ,每年有大量养分损失 ;不同作物和地区有一定差异
    19  Moleculer identification and pathogenicity detection of Pseudomonas syringae pv.actinidae
    赵利娜 胡家勇 叶振风 刘普 朱立武
    2012, 31(5):604-608. DOI: 1000-2421(2012)05-0604-05
    [Abstract](1678) [HTML](0) [PDF 1.06 M](2837)
    To identify the pathogenic bacteria of kiwifruit bacterial canker, the infected kiwifruit branches and barks from Anhui (Yuexi County) Province,Shaan’xi (Huxian County) Province and Chongqing (Qianjiang County) Municipality were collected as the experiment materials. Plate streaking, gradient dilution and beef extract peptone agar (BPA) media cultivation were used for bacteria isolation, and tobacco hypersensitive responses detection, kiwifruit pathogenicity detection and 16S-23S rDNA-ITS sequential analysis were carried out as follows for bacteria identification. From the bacterial canker infected kiwifruit tissues, 10 pathogenic strains were isolated, and all isolated strains have pathogenicity and hypersensitivity to tested kiwifruit and tobacco leaves. However, different isolated strains have different pathogenicity for kiwifruit. 10 purified pathogenic strains belonged to the same pathogen through 16S-23S rDNA-ITS and Blast sequential analysis. The amplified 280 bp sequences were the same as the previously reported 16S-23S rDNA-ITS of Pseudomonas syringae pv.actinidae. It is concluded that the isolates from the canker infected kiwifruit branches and barks were Pseudomonas syringae pv.actinidae.

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