Most cited articles
2002, 21(3).
Abstract:
根据全国农业技术推广服务中心的 2 0 0 0年度土壤肥料专业统计数据 ,分析研究了中国秸秆资源数量、利用途径及养分去向。结果表明 ,2 0 0 0年全国秸秆资源总量达 5 .5 4 1× 10 8t,其中含有的N、P2 O5、K2 O分别为4 .939× 10 6t、15 6 7× 10 6t、9.82 5× 10 6t,总养分为 1.6 33× 10 7t;在作物秸秆资源中 ,以水稻、玉米和小麦为主 ,共占76 .1% ;秸秆利用中 ,肥料、燃料和饲料量分别占秸秆资源的 36 .6 %、2 3.7%和 2 2 .6 % ,其它如原料、焚烧和弃置乱堆共占 17% ;秸秆资源中的养分利用也是以肥料量最多 ,其次是燃料和饲料 ,原料、焚烧和弃置乱堆较少 ,N、P2O5和K2 O养分还田率分别为 4 7.3%、82 .9%和 84 .4 % ,每年有大量养分损失 ;不同作物和地区有一定差异
根据全国农业技术推广服务中心的 2 0 0 0年度土壤肥料专业统计数据 ,分析研究了中国秸秆资源数量、利用途径及养分去向。结果表明 ,2 0 0 0年全国秸秆资源总量达 5 .5 4 1× 10 8t,其中含有的N、P2 O5、K2 O分别为4 .939× 10 6t、15 6 7× 10 6t、9.82 5× 10 6t,总养分为 1.6 33× 10 7t;在作物秸秆资源中 ,以水稻、玉米和小麦为主 ,共占76 .1% ;秸秆利用中 ,肥料、燃料和饲料量分别占秸秆资源的 36 .6 %、2 3.7%和 2 2 .6 % ,其它如原料、焚烧和弃置乱堆共占 17% ;秸秆资源中的养分利用也是以肥料量最多 ,其次是燃料和饲料 ,原料、焚烧和弃置乱堆较少 ,N、P2O5和K2 O养分还田率分别为 4 7.3%、82 .9%和 84 .4 % ,每年有大量养分损失 ;不同作物和地区有一定差异
2002, 21(6).
Abstract:
Based on the surveys of the absorbed dust of plants in Zhengzhou city, the effects of pure air pollution of plant were estimated by means of creating models. The green plants were classified into 5 levels by using assessment and relative factors. The gre
Based on the surveys of the absorbed dust of plants in Zhengzhou city, the effects of pure air pollution of plant were estimated by means of creating models. The green plants were classified into 5 levels by using assessment and relative factors. The gre
2005, 24(1).
Abstract:
From more than ten provinces and cities in China, 32 strains of Haemophilus parasuis were isolated from swines which characterized by polyserositis and fibrinopurulent polyarthritis, based on morphologic characteristics, cultural characteristics and bio
From more than ten provinces and cities in China, 32 strains of Haemophilus parasuis were isolated from swines which characterized by polyserositis and fibrinopurulent polyarthritis, based on morphologic characteristics, cultural characteristics and bio
2001, 20(5).
Abstract:
提取高质量的ＤＮＡ是有效开展分子生物学研究的前提。多糖污染是木本植物ＤＮＡ提取中最常遇到的问题。多糖污染的ＤＮＡ呈粘稠的胶状 ,不但给实验操作带来不便 ,更重要的是多糖对反应体系中的酶活性产生抑制作用 ,严重影响分子生物学研究的下游工作[1～3] 。很多多年生木本果树 ,即使其幼嫩组织多糖的含量也明显高于大田作物 ,所以 ,常规的ＤＮＡ提取方法因不能有效除去多糖而在木本果树应用中受到限制。经典的ＣｓＣｌ梯度离心能有效除去柑桔等木本果树的多糖而得到高纯度的ＤＮＡ ,但由于梯度离心设备昂贵 ,涉及ＥＢ ,操作不便 ,且ＤＮ…
提取高质量的ＤＮＡ是有效开展分子生物学研究的前提。多糖污染是木本植物ＤＮＡ提取中最常遇到的问题。多糖污染的ＤＮＡ呈粘稠的胶状 ,不但给实验操作带来不便 ,更重要的是多糖对反应体系中的酶活性产生抑制作用 ,严重影响分子生物学研究的下游工作[1～3] 。很多多年生木本果树 ,即使其幼嫩组织多糖的含量也明显高于大田作物 ,所以 ,常规的ＤＮＡ提取方法因不能有效除去多糖而在木本果树应用中受到限制。经典的ＣｓＣｌ梯度离心能有效除去柑桔等木本果树的多糖而得到高纯度的ＤＮＡ ,但由于梯度离心设备昂贵 ,涉及ＥＢ ,操作不便 ,且ＤＮ…
1999, 18(2).
Abstract:
The grain filling processes of eleven wheat varieties with different kernel weight planted in Hubei Province were modeled with Logistic equation. The difference of grain filling parameters among three groups of varieties with different kernel
The grain filling processes of eleven wheat varieties with different kernel weight planted in Hubei Province were modeled with Logistic equation. The difference of grain filling parameters among three groups of varieties with different kernel
2001, 20(5).
Abstract:
扩增片段长度多态性 (Ａｍｐｌｉｆｉｅｄｆｒａｇｍｅｎｔｌｅｎｇｔｈｐｏｌｙｍｏｒｐｈｉｓｍ ,ＡＦＬＰ)是近几年发展起来的新一代分子标记技术 ,自 1 995年Ｚａｂｅａｕ和Ｖｏｓ以论文形式正式发表以来[1 ] ,该方法已被广泛应用于生物多样性研究、图谱构建、基因分离、ＱＴＬ分析、品种鉴定、杂种优势预测和标记辅助选择育种等多方面的研究 ,涉及到的作物有水稻[2～ 4] 、玉米[5 ,6] 、西红柿[7] 、马铃薯[8,9] 、大麦[1 0～ 1 3] 和大豆[1 4 ] 等 ,但在油菜上还未见报道。目前几乎都使用到对人体有害的同位素标记和价格昂贵的试剂盒 …
扩增片段长度多态性 (Ａｍｐｌｉｆｉｅｄｆｒａｇｍｅｎｔｌｅｎｇｔｈｐｏｌｙｍｏｒｐｈｉｓｍ ,ＡＦＬＰ)是近几年发展起来的新一代分子标记技术 ,自 1 995年Ｚａｂｅａｕ和Ｖｏｓ以论文形式正式发表以来[1 ] ,该方法已被广泛应用于生物多样性研究、图谱构建、基因分离、ＱＴＬ分析、品种鉴定、杂种优势预测和标记辅助选择育种等多方面的研究 ,涉及到的作物有水稻[2～ 4] 、玉米[5 ,6] 、西红柿[7] 、马铃薯[8,9] 、大麦[1 0～ 1 3] 和大豆[1 4 ] 等 ,但在油菜上还未见报道。目前几乎都使用到对人体有害的同位素标记和价格昂贵的试剂盒 …
2002, 21(5).
Abstract:
The grinding quality could be improved by spraying urea solution during ripening period, meanwhile the amylose content was improved and gel consistency was extended while the chalkiness was increased. The fine quantity of nitrogen for the best quality of
The grinding quality could be improved by spraying urea solution during ripening period, meanwhile the amylose content was improved and gel consistency was extended while the chalkiness was increased. The fine quantity of nitrogen for the best quality of
2002, 21(3).
Abstract:
In this paper, we introduce the Markov model into the trend analysis and forecast about the dynamic changes of the wetland landscape in the Four Lake area was introdiced in this paper. By using the remote sensing and GIS methods and Markov model for anal
In this paper, we introduce the Markov model into the trend analysis and forecast about the dynamic changes of the wetland landscape in the Four Lake area was introdiced in this paper. By using the remote sensing and GIS methods and Markov model for anal
1999, 18(2).
Abstract:
This study showed that increasing the concentration of Cd2 and prolonging toxic time of Cd2 could reduce protein content and the activity of SOD and CAT, increase MDA content and the activity of POD, also increase the permeability of
This study showed that increasing the concentration of Cd2 and prolonging toxic time of Cd2 could reduce protein content and the activity of SOD and CAT, increase MDA content and the activity of POD, also increase the permeability of
2001, 20(1).
Abstract:
根据国外已发表的猪环状病毒2型（PCV－2）的全基因组序列，设计一对2型特异性引物，对可疑病料进行PCR扩增。将扩增产物连接到pMD18－T载体上并克隆到大肠杆菌DH5α中，经提取质粒进行PCR、酶切、测序鉴定，证明扩增出了PCV－2的目的片段。将测序结果与GenBank收录的PCV－2序列进行比较，发现同源性均在90％以上。用该PCR方法在43份可疑病料中检测到了12分PCV－2阳性病料，表明该病在我国已有流行。
根据国外已发表的猪环状病毒2型（PCV－2）的全基因组序列，设计一对2型特异性引物，对可疑病料进行PCR扩增。将扩增产物连接到pMD18－T载体上并克隆到大肠杆菌DH5α中，经提取质粒进行PCR、酶切、测序鉴定，证明扩增出了PCV－2的目的片段。将测序结果与GenBank收录的PCV－2序列进行比较，发现同源性均在90％以上。用该PCR方法在43份可疑病料中检测到了12分PCV－2阳性病料，表明该病在我国已有流行。
1994, 13(4).
Abstract:
Phenolic mixture was extract from shed leaves of Magnolia officinalis, and the action of the mixture inhibiting parasitic fungi of plant was tested on PDA medium. In addition, experiments using the extract to control plant diseases were made in potting an
Phenolic mixture was extract from shed leaves of Magnolia officinalis, and the action of the mixture inhibiting parasitic fungi of plant was tested on PDA medium. In addition, experiments using the extract to control plant diseases were made in potting an
2001, 20(5).
Abstract:
从棉花根围和根内分离到17个供试菌株在平板对峙培养中，都能极显著抑制Verticillium dahliae Kleb(简称Vd）的生长，其中15株的抑菌率大于65％，最高达89．6％。这些菌株的培养滤液也对Vd的生长有抑制作用。对供试细菌的防病及棉花出苗安全性所作的盆栽试验结果表明，13个菌株对棉花出苗表现安全；10个菌株防治棉花黄萎病效果极显著，其中4株（ICB－18、NCD－2、CS－25和CS－27）的防治效果达72．3％0－81．4％；3株（C－94、C－28和NCD－25）的防治效果鬃55．7％－61．9％，而且这7株对棉花出苗都安全。田间试验结果表明，在这7个菌株中，菌株NCD－2能够极显著地防治棉花黄萎病，防治效果为78．1％；另外4株（ICB－18、CS－25、C－94和C－28）能显著降低黄萎病病情指数，防治效果达37．1％－47．3％。
从棉花根围和根内分离到17个供试菌株在平板对峙培养中，都能极显著抑制Verticillium dahliae Kleb(简称Vd）的生长，其中15株的抑菌率大于65％，最高达89．6％。这些菌株的培养滤液也对Vd的生长有抑制作用。对供试细菌的防病及棉花出苗安全性所作的盆栽试验结果表明，13个菌株对棉花出苗表现安全；10个菌株防治棉花黄萎病效果极显著，其中4株（ICB－18、NCD－2、CS－25和CS－27）的防治效果达72．3％0－81．4％；3株（C－94、C－28和NCD－25）的防治效果鬃55．7％－61．9％，而且这7株对棉花出苗都安全。田间试验结果表明，在这7个菌株中，菌株NCD－2能够极显著地防治棉花黄萎病，防治效果为78．1％；另外4株（ICB－18、CS－25、C－94和C－28）能显著降低黄萎病病情指数，防治效果达37．1％－47．3％。
2003, 22(4).
Abstract:
在饲料中添加定量的酵母 β -葡聚糖 ,投喂经注射接种福尔马林灭活的嗜水气单胞菌 (Aeromonashydrophila)菌苗的异育银鲫 (Carassiusauratusgibelio) 2 8d后 ,通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性 (SGPT)、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率 (RPS) ,探讨了酵母 β -葡聚糖对受免异育银鲫免疫应答的增强作用。结果表明 ,投喂添加 2 0 0 .0mg/ (kg·d)酵母 β -葡聚糖的饲料 ,不仅可以提高受免异育银鲫对灭活嗜水气单胞菌的免疫应答水平 ,增强抵抗嗜水气单胞菌人工感染的能力 ,而且还具有一定的促进生长和改善肝功能的作用。
在饲料中添加定量的酵母 β -葡聚糖 ,投喂经注射接种福尔马林灭活的嗜水气单胞菌 (Aeromonashydrophila)菌苗的异育银鲫 (Carassiusauratusgibelio) 2 8d后 ,通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性 (SGPT)、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率 (RPS) ,探讨了酵母 β -葡聚糖对受免异育银鲫免疫应答的增强作用。结果表明 ,投喂添加 2 0 0 .0mg/ (kg·d)酵母 β -葡聚糖的饲料 ,不仅可以提高受免异育银鲫对灭活嗜水气单胞菌的免疫应答水平 ,增强抵抗嗜水气单胞菌人工感染的能力 ,而且还具有一定的促进生长和改善肝功能的作用。
2004, 23(5).
Abstract:
Recent studies about the nitrogen transport systems and genes related were reviewed . There are low- and high-affinity transport syste ms (LATS and HATS, respectively) in the plasma membranes of root cells for uptak ing NO 3 - -N and NH 4+ -N.
Recent studies about the nitrogen transport systems and genes related were reviewed . There are low- and high-affinity transport syste ms (LATS and HATS, respectively) in the plasma membranes of root cells for uptak ing NO 3 - -N and NH 4+ -N.
15 Influence of Different Planting Methods on Growth and Development Characteristics and Yield of Rice
2010, 29(1):1-5.
DOI: 1000-2421（2010）01-0001-05
Abstract:
Growth and development characteristics and yield formation of the rice of precision hill-drop drilling were studied comparing with manual spreading,manual transplanting.Results showed that precision hill-drop drilling shortened the total growing period of rice,improved individual and the biological production area index,and reduced the total leaf age of the main stem.The tiller of precision hill-drop drilling was strong and powerful,low-leaf tiller,tiller occurred early and the peak of tillering appeared early,and the total tiller per unit area increased as well.Effective rice panicle of precision hill-drop drilling was 263.63 panicles per m2,increased by 24.63% and 15.25% more than manual spreading and transplanting,and its actual yield was 14.92% and 4.59% higher than manual spreading and transplanting,respectively.
Growth and development characteristics and yield formation of the rice of precision hill-drop drilling were studied comparing with manual spreading,manual transplanting.Results showed that precision hill-drop drilling shortened the total growing period of rice,improved individual and the biological production area index,and reduced the total leaf age of the main stem.The tiller of precision hill-drop drilling was strong and powerful,low-leaf tiller,tiller occurred early and the peak of tillering appeared early,and the total tiller per unit area increased as well.Effective rice panicle of precision hill-drop drilling was 263.63 panicles per m2,increased by 24.63% and 15.25% more than manual spreading and transplanting,and its actual yield was 14.92% and 4.59% higher than manual spreading and transplanting,respectively.
2002, 21(6).
Abstract:
Sewage sludge is one of fertilizer which contains high organic matter?nitrogen?phosphorous and lower potassium and its application in farmland can improve the growth of plant and soil fertilizer, so the treatment of sewage sludge are showing a tendency o
Sewage sludge is one of fertilizer which contains high organic matter?nitrogen?phosphorous and lower potassium and its application in farmland can improve the growth of plant and soil fertilizer, so the treatment of sewage sludge are showing a tendency o
2002, 21(6).
Abstract:
The application of N and K fertilizer not only significantly increase the vegetable yield, but also had remarkable impacts on the quality of products. The directions and extents of the impacts were effected by the vegetable species, fertilizer rate, vari
The application of N and K fertilizer not only significantly increase the vegetable yield, but also had remarkable impacts on the quality of products. The directions and extents of the impacts were effected by the vegetable species, fertilizer rate, vari
2001, 20(3).
Abstract:
Extracts of 14 Chinese plants (belong to 10 families in Hubei,Shanxi and Guangdong Provinces, China) were assessed for nematicidal activity against Bursaphelenchus xylophilus using a fungal feeding method in vitro. Some of 14 plant extracts were also
Extracts of 14 Chinese plants (belong to 10 families in Hubei,Shanxi and Guangdong Provinces, China) were assessed for nematicidal activity against Bursaphelenchus xylophilus using a fungal feeding method in vitro. Some of 14 plant extracts were also
