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  • 1  Analysis on the Current Status of Utilization of Crop Straw in China
    Gao Xiangzhao ) ) Ma Wenqi ) Ma Changbao ) Zhang Fusuo ) Wang Yunhua )
    2002, 21(3).
    [Abstract](1726) [HTML](0) [PDF 0.00 Byte](174) [Cited by](93)
    Abstract:
    根据全国农业技术推广服务中心的 2 0 0 0年度土壤肥料专业统计数据 ,分析研究了中国秸秆资源数量、利用途径及养分去向。结果表明 ,2 0 0 0年全国秸秆资源总量达 5 .5 4 1× 10 8t,其中含有的N、P2 O5、K2 O分别为4 .939× 10 6t、15 6 7× 10 6t、9.82 5× 10 6t,总养分为 1.6 33× 10 7t;在作物秸秆资源中 ,以水稻、玉米和小麦为主 ,共占76 .1% ;秸秆利用中 ,肥料、燃料和饲料量分别占秸秆资源的 36 .6 %、2 3.7%和 2 2 .6 % ,其它如原料、焚烧和弃置乱堆共占 17% ;秸秆资源中的养分利用也是以肥料量最多 ,其次是燃料和饲料 ,原料、焚烧和弃置乱堆较少 ,N、P2O5和K2 O养分还田率分别为 4 7.3%、82 .9%和 84 .4 % ,每年有大量养分损失 ;不同作物和地区有一定差异
    2  An efficient method for genomic DNA extraction from woody fruit plants
    程运江 伊华林 等
    2001, 20(5).
    [Abstract](1214) [HTML](0) [PDF 0.00 Byte](143) [Cited by](71)
    Abstract:
    提取高质量的DNA是有效开展分子生物学研究的前提。多糖污染是木本植物DNA提取中最常遇到的问题。多糖污染的DNA呈粘稠的胶状 ,不但给实验操作带来不便 ,更重要的是多糖对反应体系中的酶活性产生抑制作用 ,严重影响分子生物学研究的下游工作[1~3] 。很多多年生木本果树 ,即使其幼嫩组织多糖的含量也明显高于大田作物 ,所以 ,常规的DNA提取方法因不能有效除去多糖而在木本果树应用中受到限制。经典的CsCl梯度离心能有效除去柑桔等木本果树的多糖而得到高纯度的DNA ,但由于梯度离心设备昂贵 ,涉及EB ,操作不便 ,且DN…
    3  Isolation and Serovar Identification of Haemophilus parasuis in China
    Cai Xuwang~ ) Liu Zhengfei~ ) Chen Huanchun~ ) Wu Bin~ ) Jin Meilin~ ) Pat Blackall~ )
    2005, 24(1).
    [Abstract](1207) [HTML](0) [PDF 0.00 Byte](112) [Cited by](68)
    Abstract:
    From more than ten provinces and cities in China, 32 strains of Haemophilus parasuis were isolated from swines which characterized by polyserositis and fibrinopurulent polyarthritis, based on morphologic characteristics, cultural characteristics and bio
    4  The Effect of Greenland on Absorbed Dust and Its Assessment Method
    Zhao Yong Li Shuren Yan Zhiping
    2002, 21(6).
    [Abstract](1457) [HTML](0) [PDF 0.00 Byte](163) [Cited by](61)
    Abstract:
    Based on the surveys of the absorbed dust of plants in Zhengzhou city, the effects of pure air pollution of plant were estimated by means of creating models. The green plants were classified into 5 levels by using assessment and relative factors. The gre
    5  Silver-Stained Afip-A Novel Assay for DNA Fingerprinting in Brassica napus
    陆光远 杨光圣 等
    2001, 20(5).
    [Abstract](1113) [HTML](0) [PDF 0.00 Byte](109) [Cited by](56)
    Abstract:
    扩增片段长度多态性 (Amplifiedfragmentlengthpolymorphism ,AFLP)是近几年发展起来的新一代分子标记技术 ,自 1 995年Zabeau和Vos以论文形式正式发表以来[1 ] ,该方法已被广泛应用于生物多样性研究、图谱构建、基因分离、QTL分析、品种鉴定、杂种优势预测和标记辅助选择育种等多方面的研究 ,涉及到的作物有水稻[2~ 4] 、玉米[5 ,6] 、西红柿[7] 、马铃薯[8,9] 、大麦[1 0~ 1 3] 和大豆[1 4 ] 等 ,但在油菜上还未见报道。目前几乎都使用到对人体有害的同位素标记和价格昂贵的试剂盒 …
    6  ANALYSIS ON THE GRAIN FILLING CHARACTERISTICS OF WHEAT VARIETIES WITH DIFFERENT KERNEL WEIGHT
    Zhou Zhuqing Zhu Xutong
    1999, 18(2).
    [Abstract](1148) [HTML](0) [PDF 0.00 Byte](116) [Cited by](53)
    Abstract:
    The grain filling processes of eleven wheat varieties with different kernel weight planted in Hubei Province were modeled with Logistic equation. The difference of grain filling parameters among three groups of varieties with different kernel
    7  The Application of the Markov Model on the Dynamic Change of Wetland Landscape Pattern in Four-Lake Area
    Wang Xuelei ) ) Wu Yijin )
    2002, 21(3).
    [Abstract](1242) [HTML](0) [PDF 0.00 Byte](125) [Cited by](51)
    Abstract:
    In this paper, we introduce the Markov model into the trend analysis and forecast about the dynamic changes of the wetland landscape in the Four Lake area was introdiced in this paper. By using the remote sensing and GIS methods and Markov model for anal
    8  EFFECT OF CADMIUM STRESS ON SEED LENG GROWTH AND PHYSIOLOGY-CHEMISTRY OF MAIZE
    Kong Xiangsheng Guo Xiupu Zhang Miaoxia
    1999, 18(2).
    [Abstract](978) [HTML](0) [PDF 0.00 Byte](117) [Cited by](48)
    Abstract:
    This study showed that increasing the concentration of Cd2 and prolonging toxic time of Cd2 could reduce protein content and the activity of SOD and CAT, increase MDA content and the activity of POD, also increase the permeability of
    9  The Establishment And Application of PCR to Diagnosis Porcine Circovirus Type 2
    Cao Shengbo Chen Huanchun Xiao Shaobo He Qigai Xu Yindi Liu Junfa
    2001, 20(1).
    [Abstract](896) [HTML](0) [PDF 0.00 Byte](122) [Cited by](48)
    Abstract:
    根据国外已发表的猪环状病毒2型(PCV-2)的全基因组序列,设计一对2型特异性引物,对可疑病料进行PCR扩增。将扩增产物连接到pMD18-T载体上并克隆到大肠杆菌DH5α中,经提取质粒进行PCR、酶切、测序鉴定,证明扩增出了PCV-2的目的片段。将测序结果与GenBank收录的PCV-2序列进行比较,发现同源性均在90%以上。用该PCR方法在43份可疑病料中检测到了12分PCV-2阳性病料,表明该病在我国已有流行。
    10  Effects of Applying Time and Quantity of Nitrogen Fertilizer on Rice Quality
    Yang Zemin Wang Weijin Cai Mingli Chen Guoxing Lu Bilin Zhu Yonggui
    2002, 21(5).
    [Abstract](1162) [HTML](0) [PDF 0.00 Byte](127) [Cited by](48)
    Abstract:
    The grinding quality could be improved by spraying urea solution during ripening period, meanwhile the amylose content was improved and gel consistency was extended while the chalkiness was increased. The fine quantity of nitrogen for the best quality of
    11  STUDY ON ACTIVE COMPONENTS IDENTIFICATION OF MAGNOLIA OFFICINALIS AND THEIR CONTROLLING EFFECTS ON PLANT DISEASES
    Zhao Chunsen Huang Junbin Zhou Maofan
    1994, 13(4).
    [Abstract](1181) [HTML](0) [PDF 0.00 Byte](192) [Cited by](44)
    Abstract:
    Phenolic mixture was extract from shed leaves of Magnolia officinalis, and the action of the mixture inhibiting parasitic fungi of plant was tested on PDA medium. In addition, experiments using the extract to control plant diseases were made in potting an
    12  Enhancement of Immune Response in Gibel Carp (Carassius auratus gibelio) Inoculated with Aeromonas hydrophila Bacterin Using Yeast β-Glucan
    Chen Chaoran ) Chen Xuan ) Chen Changfu ) Yao Juan ) Liang Yunxiang )
    2003, 22(4).
    [Abstract](1548) [HTML](0) [PDF 0.00 Byte](142) [Cited by](38)
    Abstract:
    在饲料中添加定量的酵母 β -葡聚糖 ,投喂经注射接种福尔马林灭活的嗜水气单胞菌 (Aeromonashydrophila)菌苗的异育银鲫 (Carassiusauratusgibelio) 2 8d后 ,通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性 (SGPT)、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率 (RPS) ,探讨了酵母 β -葡聚糖对受免异育银鲫免疫应答的增强作用。结果表明 ,投喂添加 2 0 0 .0mg/ (kg·d)酵母 β -葡聚糖的饲料 ,不仅可以提高受免异育银鲫对灭活嗜水气单胞菌的免疫应答水平 ,增强抵抗嗜水气单胞菌人工感染的能力 ,而且还具有一定的促进生长和改善肝功能的作用。
    13  Biological Control of Cotton Verticillium Wilt by Antagonistic Bacteria
    Li Shezeng ) Ma Ping ) Liu Xingzhong ) H. C. Huang ) Chen Xinhua )
    2001, 20(5).
    [Abstract](1057) [HTML](0) [PDF 0.00 Byte](125) [Cited by](36)
    Abstract:
    从棉花根围和根内分离到17个供试菌株在平板对峙培养中,都能极显著抑制Verticillium dahliae Kleb(简称Vd)的生长,其中15株的抑菌率大于65%,最高达89.6%。这些菌株的培养滤液也对Vd的生长有抑制作用。对供试细菌的防病及棉花出苗安全性所作的盆栽试验结果表明,13个菌株对棉花出苗表现安全;10个菌株防治棉花黄萎病效果极显著,其中4株(ICB-18、NCD-2、CS-25和CS-27)的防治效果达72.3%0-81.4%;3株(C-94、C-28和NCD-25)的防治效果鬃55.7%-61.9%,而且这7株对棉花出苗都安全。田间试验结果表明,在这7个菌株中,菌株NCD-2能够极显著地防治棉花黄萎病,防治效果为78.1%;另外4株(ICB-18、CS-25、C-94和C-28)能显著降低黄萎病病情指数,防治效果达37.1%-47.3%。
    14  Screening for Nemaicidal Activity of Some Chinese Plant Extracts against Plant Parasitic Nematodes, Bursaphelenchus xylophilus, Meloidogyne arenaria and Hirschmanniella oryzae
    Wen Yanhua Feng Zhixin Xu Hanhong Chen Li
    2001, 20(3).
    [Abstract](1394) [HTML](0) [PDF 0.00 Byte](126) [Cited by](35)
    Abstract:
    Extracts of 14 Chinese plants (belong to 10 families in Hubei,Shanxi and Guangdong Provinces, China) were assessed for nematicidal activity against Bursaphelenchus xylophilus using a fungal feeding method in vitro. Some of 14 plant extracts were also
    15  Recent Avance in Agricultural Disposal of Sewage Sludge
    Tan Qiling Hu Chengxiao Zhao Bin Chen Li Wu Lishu
    2002, 21(6).
    [Abstract](1321) [HTML](0) [PDF 0.00 Byte](168) [Cited by](33)
    Abstract:
    Sewage sludge is one of fertilizer which contains high organic matter?nitrogen?phosphorous and lower potassium and its application in farmland can improve the growth of plant and soil fertilizer, so the treatment of sewage sludge are showing a tendency o
    16  THE COMPARATIVE ANATOMY STUDIES ON DIGESTIVE SYSTEM OF 6 FISH SPECIES OF STOMACH-CONTAINING TELEOST IN FRESHWATER
    Pan Qiansheng Guo Guangquan Fang Zhiping Li Zhanguo
    1996, 15(5).
    [Abstract](1174) [HTML](0) [PDF 0.00 Byte](132) [Cited by](32)
    Abstract:
    The digestive system of 6 teleost fish species with stomach living in freshwater, Channa argus, Siniperca chuatsi, Microperus salmoides, Pelteobagrus fulvidraco, Silurus asotus, Monopterus albus, were observed by using comparative anatomy method. The morp
    17  INHIBITION OF FLY MAGGOT CHITIN ON SOME PLANT PATHOGENIC FUNGI
    赖凡 雷朝亮
    1998, 17(2).
    [Abstract](1158) [HTML](0) [PDF 0.00 Byte](125) [Cited by](32)
    Abstract:
    观察了由蝇蛆壳制备而成的几丁糖(壳聚糖),在pH5.6、1mg/ml时16种常见植物病原真菌的生长抑制作用,以及几丁糖含量分别在0.75、1.5、3.0、6.0mg/ml时对菌丝生长的抑制情况,发现几丁糖具有广谱的抑菌特性,而且随几丁糖浓度的提高,其抑制率也相应提高,但当几丁糖浓度达到3.0mg/ml以后,抑制率又保持相对稳定,从抑菌效果来看,几丁糖对丝核菌的抑制效果高,而对棉炭疽病及茄枯萎病菌则
    18  The Effect of N and K Fertilizer on Vegetable Quality
    Guo Xisheng Wu Lishu
    2002, 21(6).
    [Abstract](959) [HTML](0) [PDF 0.00 Byte](150) [Cited by](31)
    Abstract:
    The application of N and K fertilizer not only significantly increase the vegetable yield, but also had remarkable impacts on the quality of products. The directions and extents of the impacts were effected by the vegetable species, fertilizer rate, vari
    19  PRIMARY STUDY ON THE ISOLATEDmICROSPORES CULTURE OF Brassica oleracea L.
    Yan Zhun Tian Zhihongmeng Jinling
    1999, 18(1).
    [Abstract](1058) [HTML](0) [PDF 0.00 Byte](137) [Cited by](30)
    Abstract:
    甘蓝是一种重要的蔬菜作物,利用小孢子培养方法可以获得纯合的双单倍体,加快甘蓝的育种进程以及提供遗传分析的材料。目前,有关甘蓝小孢子培养国内外报道较少,而且这些研究所用的材料多为结球甘蓝和青花菜等[1,2],而有关苤蓝的游离小孢子培养还未见报道。根据前...

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