Abstract:The flanking sequence of a rice T-DNA inserted mutant G48 with a blight bacteria resistance was analyzed via PCR-walking.Results showed the T-DNA was inserted into the clone OSJNBa0076E06 (chromosome 3,AC132215.1) in Oryza sativa L.japonica cv.nipponbare.The left and right border of T-DNA inserted was 93750th and 93824th nucleotide of OSJNBa0076E06,respectively.PCR-walking provided a simple and effective method for analyzing flanking sequence of T-DNA insertion.

Abstract:In order to let kenaf cytoplasmic male sterile（CMS）lines and maintainers in southern China live through the winter safely and to realize perennial cultivation,propagation of male sterile lines will be fixed in the lower backcross generation.Kenaf cytoplasmic male sterile（CMS） line P3A and its maintainer P3B were grafted on the kenaf wild species H040,hybrids P3A×H040,and its grafing were used as controls.The results of cluster analysis and membership function showed that cold resistance may be divided into 5 categories,level 1,the most:kenaf wild species H040 and hybrids P3A×H040; level 2,strong:P3A/P3A×H040 and P3A/H040; level 3 medium:P3A CK and P3A/P3B; level 4,Less:P3B/P3A×H040 and P3B/H040; level 5,the weakest:P3B/P3A and P3B CK.Main composition analysis showed that the first principal components were withered degree,soluble sugar content,conductivity,number of leaves; The second principal component was water content of stem.Kenaf scion cold resistance on grafted by strong seedling rootstocks was improved.

Abstract:Large water-level-fluctuation zone was formed after the impoundment of the Three Gorges Reservoir.The microbial diversity of this area could be greatly influenced by the water level fluctuation.The enumeration and BIOLOG-ECO methods were used to assess the microbial diversity in the water-level-fluctuation zone.Results showed that the quantity of cultured microorganisms was highly dependent on the position.The quantity of cultured microorganisms changed over time.The data of BIOLOG demonstrated clearly that the diversity index of most soil samples did not changed significantly.The soil microbes from different soil samples utilized different carbon sources,and their ability to utilize certain carbon sources increased with time.

Abstract:This study aims to identify a Bacillus strain HNA3 isolated from the rhizosphere soil and characterize its broad antagonistic capacity towards various plant pathogenic fungi,and to optimize the conditions for the fermentation and the bioactive component separation,therefore,to establish a solid basis for developing the HNA3 strain into a promising bioactive agent with a broad spectrum of antifungal activity.In the present work,the classification status of the HNA3 was identified through morphological observation,16S rDNA sequence and the order of species-unique genes arrangement analysis.The spectrum and capacity of the HNA3’s antifungal activity was identified by the plate dual-culture antagonistic experiment and the antimicrobial inhibition rate,respectively.The flask shaking fermentation and acid precipitation method were used to determine the optimal conditions for the HNA3’s antimicrobial compound production and separation.By using the methods of surface activity examination and the analysis on the arrangement of candidate function genes and sequences,the basic properties of the bioactive antimicrobial compound were determined.The results showed that the HNA3 was identified as Bacillus amyloliquefaciens,and the BPY was the appropriate medium for HNA3’s culture growth,as well as for the production of bioactive antifungal compound.It was also found that the bioactive antifungal compound existed in the HNA3’s culture filtrates and it could be separated through acid precipitation.Further more,the bioactive compound could be ascribed to the category of lipopeptide and possessed the properties of high stability and stress resistance.In conclusion,the strain HNA3 demonstrates a broad spectrum of antifungal activity,and indicates a promising application prospective by developing it into a type of spraying,agricultural microbial fungicides in the future.

Abstract:One antagonistic bacterial strain was isolated form soli and identified as Bacillus amyloliquefaciens X-42 based on its morphology and 16S rDNA sequences.This strain produced a series of metabolites that exhibited a strong inhibitory activity to Fusarium graminearum.From its secreted metabolites during fermentation,some lipopeptides were precipitated with ammonia sulfate and used for antifungal assays.These lipopeptides were able to directly damage mycelia and conidiospores of F.graminearum,resulting in severely deformed,partly enlarged structures.Field assays showed a significant reduction of Fusarium head blight (FHB) diseases on wheat when X-42 suspension was sprayed on wheat spikes either before or after inoculation with F.graminearum.Relative protective efficacies ranged from 79% to 88%,comparable to that treated with chemical fungicide carbendazim.This study may serve as foundation for the development of new and environmental friendly biological agents to control FHB.

Abstract:A bacterial strain LM1 which can aerobically degrade sorghum liquor wastewater was isolated from the sewage water sample of a sorghum liquor manufacturer with dilution plate method.This strain was identified as Rhodococcus sp.based on its morphological observation and 16S rDNA sequence analysis.The characteristic of its liquor wastewater degradation was studied.The optimal conditions for degrading sorghum liquor wastewater were pH 7.0,temperature 30 ℃ with 5% inoculation amount.Under these conditions,(81.0 ± 2.4)% reduction in chemical oxygen demand (COD),(81.2 ± 4.1)% reduction in total organic carbon (TOC) and (66.6±0.1)% reduction in total nitrogen (TN),and (72.7±0.2)% reduction in total phosphorus (TP) of the spent wash could be achieved within 72 h by Rhodococcus sp.LM1.The value of COD and TN in effluent was (475.3±60.0) mg/L and (16.6±0.1) mg/L,which were under the highest concentration of the second discharge standard(COD≤400 mg/L) and the first discharge standard (TN≤25 mg/L) of water pollutants for fermentation alcohol and distilled spirits industry (GB 27631-2011),respectively.The effluent was further treated by Fenton oxidation as the advanced treatment,and the concentrations of COD,TOC,TN,TP and de-colorization ratio were (120.0±7.0) mg/L,(36.0±3.5) mg/L,0.1 mg/L,(13.3±0.5) mg/L and 99.8%,respectively,fitting with the standard of discharging wastewater.

Abstract:Studies of the water-holding capacity of litters and soil under the plantations of different age groups was carried out in four types of pure stands (such as Pinus massoniana forest, Cupressus funebris forest, Cunninghamia lanceolata forest and Quercus acutissima forest) in Taizishan Forest Farm,Hubei Province.The results showed that the nearly-mature stand of C.funebris forest had the biggest total litter amount（15.53 t/hm2）,the juvenile stand of Q.acutissima forest had the smallest total litter amount（2.39 t/hm2）； the maximum water-holding ratio of the litter in Q.acutissima forests of three different age groups were the highest compared with other stand types.However,the maximum water-holding capacity and effective retain capacity of the nearly-mature C.funebris stand were biggest,reaching up to 29.82 t/hm2 and 23.76 t/hm2,respectively,with middle-aged C.funebris stand,nearly-mature P.massoniana stand and nearly-mature Q.acutissima stand following it.The litters of middle-aged stand and the nearly-mature stand of C.funebris have superior effective retain capacity.The NCWHC (non-capillary water-holding capacity) and CWHC(capillary water-holding capacity) of three different-aged Q.acutissima stands below the depth of soil layer(0-40 cm) were more than others．Among the middle-aged stands and the nearly-mature stands,water-holding capacity of soil under Q.acutissima plantation was maximum（2 182.4 t/hm2 and 2 658.2 t/hm2,respectively）,water-holding capacity of soil under P.massoniana plantation was minimum（1 291.8 t/hm2 and 1 739.8 t/hm2,respectively）.

Abstract:One new species in the genus Systolederus Bolivar, Systolederus guizhouensis Deng et Zheng sp. nov. is described from eastern and southern in Guizhou,China. The type specimens are deposited in the Institute of Zoology,Shaanxi Normal University,Xi′an,China.Systolederus guizhouensis Deng et Zheng sp. nov. (Fig.1)The new species is allied to Systolederus guposhanensis Deng，Zheng et Wei,2007,but differs in: 1)width of longitudinal furrow equal to width of first segment of antennae; 2)pronotum 5.0 times as long as portion surpassing apex of hind femur; 3)wings reaching apex of pronotum; 4) width of midfemur of female equal to width of tegmina.Length of body:♂7.0~7.5 mm,♀8.0~8.5 mm； length of pronotum:♂7.5~8.0 mm,♀ 9.0~9.5 mm；length of hind femur: ♂ 4.5~5.0 mm, ♀5.0~5 .5 mm.Holotype ♀,Guizhou Ceheng (Rongdu),105.8°E, 24.9°N, alt.900 m, 2012-Ⅶ-21, collected by ZHANG Guang-hai. Paratypes: 2♂2♀,same data as holotype, collected by ZHANG Guang-hai and WU Zhao-rong; 1♀,Guizhou Shibing (Shanmuhe), 108.1°E, 27.1°N, alt.950 m, 2012-Ⅷ-09, collected by DENG Wei-an .Etymology:The new species name is from the type locality Guizhou,China.

Abstract:A new PCB-degrading rhizobacterium strain named A1 was isolated from the long-term PCBs-contaminated alfalfa rhizospheric soil by enrichment culture. Through morphological observation and sequence analysis of 16S rDNA, the strain was identified as Pandoraea norimbergensis. GC-MS was used to determine the ability of resting cells degradation of PCBs, and it was found that the strain could totally remove 56.7% of the Aroclor1242 in 72 hours. The bphA1 gene core，encoding the catalytic site of the large subunit of biphenyl dioxygenase，was sequenced and its third structure was also predicted. It showed that the nucleic acid sequence of bphA1 gene had 100% homology compared with Pandoraea sp.JB1,and the third structure of protein had 99.37% identity compared with Comamonas testosteroni B356.

Abstract:A bacterial strain HA-01, isolated from rhizosphere soil, exhibited extensive antagonistic effect on 12 fruit and vegetable pathogens. The strain was identified as Bacillus amyloliquefaciens based on morphological observation, physiological and biochemical characteristics,16S rDNA sequence homology and specific PCR detection. The effects of different treatments of strain HA-01 against disease caused by Penicillium digitatum Sacc. on inoculated mashui mandarin were also investigated. The results showed that the incidence of citrus green mold of mashui mandarin treated by cell culture,cell-free filtrate and cell suspension of strain HA-01 was less than 20% in all treatments while the incidence of control was 94.44%, and the lesion diameter was all below 2.00 cm while the lesion diameter of the control was 3.60 cm.

Abstract:Ratoon stunting disease (RSD) of sugarcane (Sacchrum spp. Hyb.), caused by bacterium Leifsonia xyli subsp. xyli (Lxx), is one of the most important economically damaging diseases worldwide. The objective of the present study was to determine the effects of RSD on plant growth and the endogenous hormone levels in sugarcane stalks. Two mechanical cane varieties (i.e. sugar cane variety of ROC22 and chewing cane variety of Badila) were selected as the plant materials and RSD infection was determined by using PCR reaction. The seedcanes were grown in pots,and three treatments were done: pathogen-free seedcanes as control; seedcanes with thermal treatment: RSD infected seedcanes were treated at 52 ℃ for 30 min; RSD infected seedcanes. During the experiment, the plant heights were investigated, and the endogenous hormone levels in cane stalks were analyzed after planting in 90,120,150 and 180 d, respectively. The results showed that: the plant heights of the RSD infected seedcanes were significantly lower than those of the control and the thermal treatment.The contents of GA3 and IAA were obviously lower in the RSD infected seedcanes than those in the control and the thermal treatment in contrast to the content of ABA which was higher in the infected seedcane treatment as compared with the control and the thermal treatment; two cane varieties showed the same patterns and there was no significant difference between the control and the thermal treatment in plant height and endogenous hormone levels. Therefore, the thermal therapy is efficient to control RSD in sugarcane.

Abstract:A bacterial strain HZ-2 was newly isolated by enrichment culture from the activated sludge in a sewage treatment plant．Based on the morphology，the physiological and biochemical characteristics，and 16S rDNA sequence analysis，the strain HZ-2 was identified as Bacillus pumilus HZ-2．The strain HZ-2 could not only completely degrade mesotrione 200 mg/L in 5 d，under the conditions of 1% inoculum, 30 ℃, pH 7.0 and 180 r/min, but also could tolerate and efficiently degrade mesotrione of high concentrations up to 400 mg/L. All these results indicate the strain HZ-2 is deserved to be further developed as a candidate mesotrione-degrading microbe.

Abstract:To provide scientific experience for higher efficient utilization of soil potassium,effects of low-molecular weight organic acids and cations on the release of potassium from different potassium-bearing minerals (biotite,muscovite and orthoclase) by the ways of extracting.The results showed that different low-molecular weight organic acids and cations increased the release of potassium from potassium-bearing minerals significantly.Compared to that of the control treatment (H2O),the release amount of potassium from biotite using oxalic acid (OA),citric acid (CA),tartaric acid (TA),NH4Ac,CaCl2,NaCl and H+ as extractants was increased up to 188.2%,151.6%,149.6%,24.6%,32.6%,3.5% and 169.8%,respectively.The release amount of potassium from muscovite using the same solutions as extractants was increased up to 211.0%,202.8%,199.4%,81.6%,38.3%,21.7% and 169.1%,respectively.The release amount of potassium from orthoclase was increased up to 219.0%,206.6%,216.0%,144.1%,27.5%,60.4% and 210.9%,respectively.Using the same extractant,the release amount of potassium was in the order of muscovite>bitotite>orthoclase.The release amount of potassium from potassium-bearing minerals was increased with the increase of the concentration of the extractant.

Abstract:Pot experiments were conducted to investigate the effects of co-application of molybdenum (Mo) and selenium (Se) on the yield,the uptaking of Mo and Se,and the quality of Chinese cabbage.The results showed that co-application of Mo and Se could increase the yield of Chinese cabbage.The concentrations and accumulations of Mo and Se,contents of Vc,contents of soluble sugar and soluble protein of Chinese cabbage were increased.But the content of nitrate of Chinese cabbage was decreased.The interaction between Mo and Se had a significant influence on the uptaking of nutrition and the quality of Chinese cabbage.Co-application of them was useful for producing Mo-riched and Se-riched Chinese cabbage.

Abstract:To improve lignocellulose energy-oriented use efficiency of the rice straw，rice straw was pretreated with phosphoric acid-acetone.The effects of particle size,materials to liquid ratio,temperature and time on enzymatic saccharification ratio of rice straw were studied.The construction of the rice straw was scanned before and after the pretreatment by SEM.The results showed that when the particle size of rice straw was ≤380 μm,the materials to liquid ratio was 1∶8,the pretreated temperature was 80 ℃ and the pretreated time was 1.5 h,the enzymatic saccharification ratio of rice straw increased from 18.6% to 65.4%.The crystalline structure of the rice straw was destroyed to pieces by SEM,providing good working conditions for the subsequent enzymatic saccharification.The research could provide an effective pretreated way of straw under mildness conditions.

Abstract:The water quality of Honghu Lake between 2007 and 2010 was taken as an example and the geostatistics and five commonly used interpolation methods (radial basis function,inverse distance weighted,global polynomial,local polynomial and ordinary Kriging) were used to analyze the temporal and spatial conditions of water quality of Honghu Lake.Error evaluation factors (MAE,MRE and RMSE) were employed to evaluate the fitting situation.It concluded that all factors of the water quality had high spatial-temporal heterogeneity and the concentration of total nitrogen was influenced by temperature,water depth and water cycle.Among the five kinds of interpolation methods,the radial basis function had a better predict result.The total nitrogen concentration of Honghu Lake had different characteristics of spatial distribution.

Abstract:A total of 21 crossbred (Duroc×Landrace×Large white) weaned piglets with an average body weight of (12.22±1.27) kg were randomly assigned into 3 groups with 7 replicates per group and 1 piglet in each replicate．The 3 groups were as the following：basal diet (groupⅠ),basal diet+2％ bamboo carbon (groupⅡ) and basal diet + bamboo vinegar 1％(group Ⅲ)．The experiment lasted for 37 d．This experiment was conducted to study effects of bamboo-carbon and bamboo vinegar on growing performance,physiological and biochemical indexes of blood and antioxidant capacity of weaned piglets.The results showed that：(1)There were no significant differences of final weight,ADG,ADFI,the ratio of feed to gain between groupⅡ,Ⅲ and groupⅠ(P>0.05).Compared with groupⅠ,final weight,ADG,ADFI in group Ⅲ,had a ascendant trend,increasing with 9.70%,14.74%,6.33% (P>0.05),respectively,while the ratio of feed to gain had a downtrend (P>0.05),decreasing by 7.17%.(2) There were no significant differences of hematologic indexes between groupⅡ,Ⅲ and groupⅠ,but the WBC,RBC,HGB,HTC,PLT of group Ⅲ were higher than that of groupⅠ.(3) On day 15 of the experiment,the activity of LDH and AST of groupⅡ was significantly higher than that of groupⅠ(P<0.05),but there was no significant difference between group Ⅲ and groupⅠ．There were no significant differences of the other index.(4）The T-AOC of group Ⅱ and Ⅲ was significantly higher than that of groupⅠ(P<0.05) on day 15 of the experiment.There was no significant difference of MDA between groupⅡ,Ⅲ and groupⅠ,but decreased by 27.68% in group Ⅲ compared with groupⅠ,and was significantly lower than in group Ⅱ.In conclusion,the effects of bamboo-carbon and bamboo vinegar on weaned piglets were more obvious in the early growing period (1-15 d) than in the later growing period (16-37 d).Addition of 2% bamboo-carbon may be too high and the addition of 1% bamboo vinegar can improve the growth performance,physiological,biochemical indexes of blood and antioxidant performance of weaned piglets.

Abstract:The aim of this study was to investigate the content of IgA,IgM and lactoferrin,and their variation affected by somatic cell count (SCC) in milk.Milk from dairy farms in Beijing,Chuzhou,Harbin,Hohhot,Urumqi,Xi’an was collected in spring and summer.The content of IgA,IgM and lactoferrin and SCC in milk were determined.Data was processed by SAS 8.0 and descriptive statistics,correlation analysis,and variance analysis (effects of season and dairy farm on IgA,IgM and lactoferrin) were carried out.The results showed that the content of IgA,IgM and lactoferrin in milk obeyed lognormal distribution.Significant differences of IgA,IgM and lactoferrin content and SCC in milk from different farms were found.The IgM and lactoferrin content from samples collected in spring and summer differed remarkably,but the IgA and SCC data was similar.Correlation coefficients between IgA,IgM,lactoferrin content and somatic cell score (SCS) were significantly positive.Content (log) of IgA,IgM,lactoferrin in milk raised with the increasing of SCS.It can be concluded that significant positive correlation existed between IgA,IgM,lactoferrin content and SCC,and content of IgM and lactoferrin in milk were affected by season.

Abstract:The spermatogenesis associated 4 (SPATA4) gene from testis of Wuchang bream (Megalobrama amblycephala) were cloned using RACE (rapid amplification of cDNA ends) technology and characterized. The full length cDNA of SPATA4 was 1 076 bp including a 678 bp open reading frame,which encode a putative protein of 225 amino acids residues with a theoretical molecular weight of 25.72 ku and an isoelectric point of 9.32. Further analysis of SPATA4 sequence indicated that it had high identity with other vertebrates. Quantitative real time PCR was performed to detect the SPATA4 mRNA in different tissues of adult fish and various developmental stages. The results showed that during 1-206 h post-fertilization,the SPATA4 gene had the highest expression levels at 4 h and 28 h,decreased at 50 h to the lowest level,and had similar expression levels at other time points . In the ten tissues tested,the SPATA4 gene showed highest expression level in testis.

Abstract:The dominant bacterial strain,designated as Z1,was isolated from hepatopancreas of diseased Danio rerio.The biochemical and physiological characteristics of the isolated strain was studied by using conventional method,such as ATB Expression System (ID32 STREP),16S rRNA and gyrB gene sequence.The results confirmed that the strain Z1 was Edwardsiella tarda. The challenge tests were carried out by artificially infecting D.rerio and Puntius tetrazona with the pure culture of the isolated strain.The symptoms of the artificially infected fish were similar to those infected naturally and the characters of the re-isolated strains were the same as the initially isolated strain.Antibiotic sensitivity test showed that among 17 antibiotics tested 11 were sensitive and 6 were resistant.The antibacterial activity of 15 Chinese herbs to pathogenic E.tarda (Z1) was determined in vitro by two fold dilution method.The results showed that Galla chinensis and Pericarpium granati were found to have significant inhibition on the bacterium with both minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) less than 6.25 mg/mL; Artemisiae argyi,Catsia tora,Prunus mume Sieb. et Zucc.,Pulsatilla chinensis,Coptis chinensis Franch.，Rheum palmatum L.，Scutellariae baicalensis Georgi showed some extent inhibition on the bacterium with MIC and MBC of 25-200 mg/mL,but Chrysanthemum indicum L.,Lonicera japonica,Astragalus membranaceus (Fisch.) Bge,Glycyrrhiza glabra,Sophorae flavescentis and Phellodendri chinense Scheeid had no inhibition on the bacterium with MBC of more than 200 mg/mL.

Abstract:To detect doxycycline (DC) residues in aquatic products,a ciELISA method were developed through preparing monoclonal antibody (McAb) by immunizing BALB/C mice,using doxycyline-bovine serum albumin conjugate (DC-BSA) as immunogen.The BALB/C mice used in the experiment for cell fusion were selected by two methods,the indirect ELISA and the indirect competitive ELISA.A hybridoma cell strain (3D1-H4),which can secret a McAb that specifically reacted with DC,were obtained.The McAb was separated by using hybridoma technology,and it was identified to belong to IgG1 by the indirect ELISA.A ciELISA for detecting DC was then developed,using this McAb.This McAb was characterized on its sensitivity,IC50 and veracity.The results showed that the titers of the McAb were 1∶（8×102） and 1∶（5.12×105） in supernatant and ascites,respectively.This McAb had 8.87%,5.86% and 2.74% cross-reactivity with tetracycline,oxytetracycline and chlotetracycline,respectively.The linear detection range for this ciELISA varied from 1.58 to 199.53 ng/mL,with a sensitivity of 2.88 ng/mL and an IC50 of 36.31 ng/mL.The recoveries of DC spiked in fish muscle and liver were 85.13% and 79.69%,respectively.Both the inter-assay and intra-assay coefficient variations were less than 15%.These results suggested that this ciELISA method was able to be used for detecting DC residues in aquatic products.

Abstract:In order to understand the water quality condition of Dacha Lake in Wuhan,composition of phytoplankton in the lake was investigated from June 2010 to May 2011.A total of seven phyla,59 genera and 89 species of phytoplankton were identified from qualitative and quantitative samples.Based on the species and quantity of phytoplankton,biological assessment of water pollution and eutrophication condition was conducted by using the methods of indicator organism and biotic diversity index (Margalef and Shannon-Weiner index).In the phytoplankton community,Chlorophyta and Cyanophyta were dominant in both alga species and quantities,followed by Bacillariophyta.Of the pollution-indicating phytoplankton,the algae indicating α-ms and β-α-ms types of eutrophication were dominant,accounting for 48.72% and 23.08% of the total,respectively.The Margalef index and Shannon-Weiner index for phytoplankton ranged from 1.45 to 2.19 and from 1.64 to 2.95,respectively.The results indicated that the water quality of Dacha Lake was of moderate pollution (α- or β- moderate pollution),and the trophic status was in the state of eutrophication (α-ms or β-α-ms),mainly in the type of α-ms as revealed by the pollution-indicating algae.

Abstract:The free amino acids and nucleotides were analyzedinraw and cooked silver carp (Hypophthalmichthys molitrix) musclesof different body weight (1.0-3.0 kg) using TAV (taste active value) and EUC (equivalent umami concentration) methods.The results showed that Tau,His and Gly were the main free amino acids in silver carp muscle,and their concentrations decreased after cooking.Histidine significantly contributed to the bitter taste,Glu contributed to the major umami taste,while Gly and Ala contributed to the sweet taste.The nucleotide concentrations in fish muscles of different body weights mainly dominated by IMP and HxR,and there were significant differences in nucleotide concentrations between raw and cooked muscles.The TAV of IMP and AMP decreased after cooking,while the TAV of Hx increased,and the major umami taste was still contributed by IMP.The EUC showed a trend of increasing followed by decreasing,with increasing body weight.And it reached the maximum (31.57 g MSG/100 g) at 2.0 kg for cooked fish muscle.

Abstract:A remote control traction monorail transporter 7YGDQ-50 was designed specifically for the complex mountainous environment. The transporter is composed of the electric hoist, the telecontrol, the control box, the driving wheels, the wire rope, the guide wheel, the travel switch, the trailer car, the track and the adjustable counterweight device, etc. To solve the problems of the present traction monorail transporter, the key components, which include the electric hoist, the control box, the driving wheels, the trailer car and the adjustable counterweight device, were redesigned and improved by the change of the driving mode. The transporter can move uphill, downhill or park by the telecontrol or a manual button. The field experiments show that the transporter improves the work efficiency compared with the existing traction monorail transporter, and is suitable for the complex mountainous transport operations. While the telecontrol is used, the receiving device should be in visual range.

Abstract:The activity sounds of Sitophilus zeamais adults in wheat, corn and Sitophilus oryzae larvae in coix seed were respectively sampled in different temperatures, and decomposed by wavelet packet. Three characteristics of decomposition nodes were analyzed. They were nodes energy, nodes energy ratio and energy entropy. The research found that the nodes energy and nodes energy ratio of the activity sound of Sitophilus zeamais adults in wheat and corn were greatly different in the same temperature, and the difference of frequency distribution between these two types of signals was obtained. It was also found that there was much difference between the energy entropy of these two types of signals, and the activity acoustic signal energy entropy mean statistical data of Sitophilus zeamais in wheat and corn were collected. In different temperatures, the nodes energy, nodes energy ratio and energy entropy of the activity sound of Sitophilus oryzae larvae in coix seed were also different. The results show that both the difference of temperature and grain species affect the activity sound characteristics of grain insects, and the activity sounds in wavelet packet decomposed characteristics are significant different.