Abstract:

Abstract:Based on the amino acid sequence of a single-chain antibody specific for zearalenone mycotoxin and codon usage in E.coli,a variable heavy chain(VH) and a variable light chain(VL) were synthesized by PCR to create a single-chain(scFv) antibody gene,ZEN2,th

Abstract:The expression of Arabidopsis Ran2 mRNA were analysed in different tissues by RT-PCR,and the direct immunofluorescence microscopy was used to determine the subcellular localization of plant Ran proteins during the cell cycle.The results showed that Ran2 i

Abstract:通过设计1对PCR引物（上游引物,5-′GCCATATGGTGCCGATCCAAAAAG-3′,下游引物,5-′GAGAATTCCCTTCAAGGCCTCAG-3′）,采用PCR方法扩增出人肥胖基因编码成熟肽的核苷酸序列,将扩增产物从琼脂糖凝胶上回收后插入测序载体pMD-18-T后进行了核苷酸序列测定。将经EcoRⅠ和NdeⅠ双酶切后的扩增产物插入经EcoRⅠ和NdeⅠ双酶切后的大肠杆菌表达载体pET-28a（＋）,构建了可在大肠杆菌中高效表达人瘦蛋白的表达载体。将表达载体转化入大肠杆菌菌株BL21,经IPTG诱导后进行SDS-PAGE检测,结果表明构建的人肥胖基因表达载体得到高效表达,重组蛋白占到菌体总蛋白的31.2%。

Abstract:Photo-thermo-sensitive genic male sterile lines,M113S,Hua201S and Peiai64S were sown continuously with interval 10~15 days and ratooned under two different natural ecological conditions in Wuhan and Hainan.Pollen sterility and seed setting rate alteration

Abstract:In the present paper,one new species of Omocestus is described.Type specimens are deposited in the Institute of Zoology,Shaanxi Normal University.Omocestus nigritibialis sp.nov.(Fig.1)The new species is similar to Omocestus petraeus(Brisout-Barnoville),18

Abstract:Thirty-nine plant methanol extracts from thirty-eight Chinese traditional medicinal plants collected from Guangxi Zhuang Autonomic Region were screened for their antifungal activity against five important plant pathogenic fungi in vitro.The tested concent

Abstract:11 pathogenic isolates and 11 nonpathogenic isolates of Phytophthora capsici were obtained from peppers and soil.The genetic diversity of all 22 isolates was assessed by RAPD,sequence analysis of the rDNA internal transcribed spacers,and pathogenicity tes

Abstract:On the basis of the investigation on the larvae of the pear leafecuring(Dasinenra pyri Bouche) in the pear garden,the aggregation index,Iwao Equation and Taylor Power-law model were applied in the analysis of spatial distribution pattern on a pear tree an

Abstract:Effects of nitrogen and potassium fertilizer applications on yield and occurrence of major diseases and insect pests of rice were studied by using the rice variety Zhong 9 You 288.The results showed that the major diseases and insect pests of rice,such as

Abstract:The relationship between the membrane structure and the nutrient release of fertilizers coated by organic polymer was studied with electron microscope.The structure and thickness of membrane in coated urea and coated ammonium phosphorus(MAP)were determine

Abstract:Effect of urea formaldehyde fertilizer on the content of chlorophyll in the canopy leave of Dongting Biluochun Tea plant was studied in order to improve the Biluochun Tea quality and the fertilizer utilization around the Tai Lake at Jiangsu Province.The r

Abstract:研究了保存时间、温度和抗冻剂对大肠杆菌感受态细胞保存的影响。结果表明：在4℃-、40℃、-70℃条件下,大肠杆菌感受态细胞转化效率在保存前期随保存时间增加呈上升趋势,转化效率在第1天或第7天达到最大值,此后随保存时间增加而呈下降趋势;在保存前期,保存于4℃的大肠杆菌感受态细胞转化效率高于保存在-70℃、-40℃的大肠杆菌感受态细胞的转化效率,在保存后期低于保存在-70℃、-40℃的大肠杆菌感受态细胞的转化效率;在相同保存温度和保存时间下,保存在甘油的大肠杆菌感受态细胞转化效率高于保存在DMSO的大肠杆菌感受态细胞的转化效率。

Abstract:The head kidney macrophages of yellow catfish(Pelteobagrus fulvidraco) were separated by Percoll continuous density gradient centrifugation,and cultivated in L-15 medium with 0.1% fetal bovine serum.The morphological and ultrastructural features,as well a

Abstract:通过测定不同规格（全长15.2-95.5 mm）、不同水温（21.2℃、16.8℃）和一天当中匙吻鲟（Poly-odon spathula）仔稚鱼饱满指数的变化,研究了匙吻鲟仔稚鱼摄食特性。结果表明,在平均全长达到20.1 mm之前（约开口摄食3 d）,随着个体的生长,饱满指数逐渐增大并在20.1 mm达到最大值,且在规格相近的个体之间,饱满指数相差较大,最高达78.4倍。平均全长达到20.1 mm以后,随着个体的生长,饱满指数呈现下降的趋势,且在规格相近的个体之间,饱满指数相差较小。水温从21.2℃降到16.8℃,20.0-23.2 mm的仔鱼饱满指数降低53.3%。仔鱼具有明显的昼夜摄食节律,属晨昏摄食,全天出现1个摄食高峰和1个摄食低谷,从低谷到高峰,饱满指数呈强弱交替波动性上升。以＂时平均饱满指数法＂计算匙吻鲟仔鱼（16.3-21.0 mm）的日摄食率为6.5%。

Abstract:Yellow cheek,Elopichthys bambusa,was collected from the Yangtze River bank ponds in Tuanfeng County,Hubei Province.The content of crude protein,fat,water and amino acid contents were measured and caloric value was calculated.The results showed that water

Abstract:为了探讨克氏原螯虾（Procambarus clarkii）暴发性疾病的传染途径和致病病原,取呈现暴发性疾病典型症状的克氏原螯虾的组织与内脏,制作成螯虾肉糜后分为3份,分别采用抗生素除菌、不做任何处理和在100℃水浴锅中保持1 h等3种方法将螯虾肉糜处理后,连续投喂体重（5.6±0.3）g的幼年虾、体重（17.5±0.4）g的青年虾和体重（24.6±0.5）g的成年虾7 d后,继续饲养观察28 d,比较了各试验组螯虾的死亡率。结果表明,口服经过抗生素除菌和未经任何处理的患病螯虾组织能导致健康试验螯虾发病而死亡,而且青年虾和成年虾死亡数量较幼年虾多;与加热处理患病螯虾组织相比,未经处理的患病螯虾组织诱导健康克氏原螯虾发病率最高,抗生素处理组其次,这种结果预示着患病螯虾组织中存在能导致克氏原螯虾疾病发生的致病性病毒和细菌,而且健康的克氏原螯虾可以通过摄食带病原的患病虾受感染。采用盐酸多西环素对患病虾的治疗未显示出疗效。

Abstract:转化子的高效筛选与再生是获得转基因植株的关键。为快速有效地筛选出转化子,并尽可能在较短时间内获得纯合的转基因植株,开展了柑橘胚性愈伤组织的转化研究。以椪柑、冰糖橙、默科特橘橙等胚性愈伤组织为外植体,采用根癌农杆菌介导法进行转化试验,根据愈伤组织再生过程中不同发育阶段的组织对抗生素的敏感程度及在不同筛选压下抗性组织的生长情况,提出了＂5步筛选再生法＂,获得了大量的再生细胞系,并经GUS检测证实为转化子。

Abstract:采用CAPS（cleaved amplified polymorphic sequence）分子标记对通过非对称原生质体融合获得的2个组合体细胞杂种植株进行了胞质遗传分析。结果表明,在伏令夏甜橙（Citrus sinensis）＋默科特橘橙（C.reticulata×C.sinensis）组合中,分析的2棵植株胞质DNA都来自伏令夏甜橙（供体）;在丹西红橘（C.retic-ulata）＋佩琦橘柚（C.reticulata×C.paradisi）组合中,分析的3棵植株胞质DNA都来自来源于佩琦橘柚（受体）。

Abstract:The effects of drought on photosynthetic characteristics in two pepper(Capsicum annuum L) cultivars were studied.The results showed that drought decreased the net photosynthetic rate(Pn),apparent maximum photosynthetic rate(Ps),potential maximum photosynt

Abstract:To identify the medicinal plants of Sedum L.,including Sedum emarginatum Migo.,S.kiangnanense D.Q.WAN,S.ellacombianum Praeger.,S.lineare Thunb.and S.sarmentosum Bunge.,by RAPD in molecular level,and preliminarily analyze the relationship among those speci

Abstract:通过典型样地法,调查了武汉市马鞍山半自然群落的物种组成、数量特征,调查结果表明：其物种共计16个群落,分属于13个群系,5个植被型和3个植被型组;共计168种植物,分属于83科149属;乔木中常绿落叶比为1.0∶2.7;生活型结构中以高位芽植物为优势,占59.51%;乔木层主要树种有马尾松、枫香、柏木,灌木层主要树种有槲栎、樟树、冬青;群落中,更新良好的种有樟树、槲栎、冬青等,更新不足和无更新的种有马尾松、柏木、枫香;群落中衰退种有马尾松、柏木等,巩固种有冬青;在城市园林应用中值得推荐的植物种有樟树、深山含笑、桂花、朴树、冬青、檵木、女贞等。

Abstract:To compare combustibility differences of woody plants and provide theory basis for the correct developing biological fire-prevention,combustion experimentations of living branches and leaves of 4 types of bayberry species and 2 types contrastive combustib

Abstract:Volatile compounds of cooked sliver carp were extracted by solid phase microextraction(SPME) with PA,CAR/PDMS and DVB/CAR/PDMS fibres were analyzed by GC-MS.The characteristics of volatile compounds of the three fibres were analyzed,and an appropriate fib

Abstract:In this paper,the multi-line detection arithmetic and its characteristics have been described based on Hough transformation principle.The characteristics include the corresponding of line & peak value,the uncertainty of multi-line detection for above-zero

Abstract:Characteristic parameters such as the peak resonant frequency,the values of the power spectrum,the power spectrum swing of the high frequency range and the ratio of the area of power spectrum between front and back on the 32 points in spectrum were found

Abstract:从3个方面总结了秀丽隐杆线虫在抗性基因方面的研究进展。秀丽隐杆线虫的先天性免疫系统在抗病原菌、抗氧化应激等方面至关重要,它主要是通过MAPK、DAF-2、TGF-β三条信号途径发挥作用;另外,含TIR区蛋白和抗菌多肽也是秀丽隐杆线虫抗微生物感染机制的重要组成部分。在秀丽隐杆线虫的抗药性基因研究方面,发现了许多抗药性相关蛋白,如P-糖蛋白和多药耐药性相关蛋白MRP在抵抗多种药物的过程中发挥重要作用;而抗驱虫剂相关蛋白也在专门针对各种驱虫剂作用机制的研究中被发现;转录因子SKN-1、信息沉默调节蛋白SIR2、谷胱甘肽转移酶等在抗百草枯等药物方面发挥着重要作用;研究还发现遍在蛋白Phb-2等对特定药物起抗性作用。此外,本文还对抗环境刺激涉及的基因、蛋白及信号途径进行了简单叙述。