LIU Yun XIONG Jia-jun YANG Li-guo
2008, 27(4).
Abstract:Gateway克隆技术现已广泛用于cDNA文库构建.然而,用常规DNA测序程序对Gateway cDNA进行测序时,反向重复的att序列易形成发夹结构,导致测序质量较差,且测得的序列较短,成为Gateway克隆技术应用的限制因素.本研究报道了1种Gateway cDNA克隆高温变性测序方法,即在Gateway cDNA测序PCR反应之前,先将DNA在98℃预变性5 min,可有效防止测序峰值的快速下降,极显著地增加Gateway cDNA克隆测序长度(质量Q≥20的序列平均长度从528 bp提高到816 bp).该方法在常规DNA测序程序的基础上仅新增了5 min的高温预变性,不增加任何实验成本和操作步骤,非常适合Gateway质粒DNA的大规模测序.
ZHANG Xi-juan LI Wei-juan LI Hong-jiao XU Zheng-jinCHEN Wen-fu ZHANG Wen-zhong ZHAO Ming-hui
2008, 27(4).
Abstract:The characteristics of growth and development and yield formation of super rice Shennong 265 were studied by using Liaojing 294 and Laojing 371 as check varieties.The results revealed that the yield of Shennong 265 was 10.20 t/hm2,11.72% and 17.38% higher
曹萍 , 吕文彦 , 程海涛 , 邵国军 , 张鉴 , 武翠 , 刘涛 , 徐兴伟 , 尹长斌
2008, 27(4).
Abstract:In order to theoretically define relationship between rice taste value and texture of brown rice of developed varieties in Liaoning Province in different years,30 varieties with erect-panicle or curved-panicle were used to analyse taste quality and full b
2008, 27(4).
Abstract:On the basis of resistance monitoring of oriental fruit fly Bactrocera dorsalis(Hendel) in 2005,the resistances from populations of Guangdong,Guangxi,Hainan,Fujian,Yunnan Provinces to three insecticides were determined again by vial residue in laboratory
CHENG Zhi-hui ZHANG Jing MENG Huan-wen
2008, 27(4).
Abstract:The influences of tomato seed coated with fungicides of carbendazim,chlorothalonil,previcur,sandofan,streptomycin and CuSO4 on seed germination,seedling growth and disease control were investigated.Seed germination rate,seedling height,root length and fre
2008, 27(4).
Abstract:Alternanthera philoxeroides(Mart.) Griseb.is a noxious exotic weed species.The occurrence and infestation of Alternanthera philoxeroides in garlic fields constitutes a major threat in garlic production.To determine the influence of Alternanthera philoxero
QIN Xiao-ping ZHANG Ya-ping YANG Mei-lin
2008, 27(4).
Abstract:小菜蛾(Plutella xylostella L.)是世界性十字花科蔬菜重要害虫.由于其发生世代重叠,加之长期使用单一化学药剂,导致小菜蛾抗药性不断增强[1-3].寻找小菜蛾防治新途径成为当前蔬菜生产急待解决的问题.蜡蚧轮枝菌(Verticillium leca-nii)是一种重要的虫生真菌,毒素是该菌感染并致死昆虫的主要原因[4].国内外对蜡蚧轮枝菌可能含有的驱避作用成分还很少有人报道.笔者从昆明罹病蚜虫分离的蜡蚧轮枝孢菌株KM9803菌株粗提物对小菜蛾的驱避作用进行了测定,旨在为利用该菌防治害虫提供科学依据.
WANG Tian-wei LI Zhao-xia SHI Zhi-hua WEN Li-hua CAI Chong-fa
2008, 27(4).
Abstract:Affected by metropolitan area,the land use changes of metropolitan fringe region were different from those of ordinary cities.The spatial-temporal features of land use changes and their driving forces were analyzed at the farm,town and region scale level
2008, 27(4).
Abstract:Three major species of Bacillus cereus group are Bacillus anthracis,Bacillus cereus,and Bacillus thuringiensis.Until now,8 whole genomes of this group have been sequenced and the data can be downloaded from NCBI.16S rDNA is a "gold standard" in the field
2008, 27(4).
Abstract:The type of the receptor of follicular inhibin was identified through isolation and purification,which would be used as the base for further study on inhibin mechanism.The pituitary of 30 sows of Landrace breed was collected and the membrane protein was p
梅仕林 , 孟宪荣 , 栗绍文 , 何诚 , 杨琪 , 喻翠翠 , 张望 , 毕丁仁
2008, 27(4).
Abstract:The object of this study is to obtain the recombinant polymorphic outer membrane POMP 90 protein of swine Chlamydophila abortus(CA) for development of diagnostic ELISA method.pomp90 gene of swine CA strain CP/12 was obtained by PCR amplification and clone
2008, 27(4).
Abstract:Grass carp,Ctenopharyngodon idellus were inoculated with formalin killed Flavobacterium columnare and were fed with feed supplemented with appropriate amount of immunopolysacchride.28 days after the inoculation,the infulence of dietary immunopolysacchride
2008, 27(4).
Abstract:Purified immunoglobulin(Ig) was isolated from the serum of European eel by using protein A sepharose affinity chromatography.The polyclonal rabbit anti-eel IgM antiserum prepared by the purified Ig was then used to detect the localization and distribution
2008, 27(4).
Abstract:Mandarin fish is of great importance in the aquaculture of Guangdong Province,which has high value for food,and is welcomed by consumers from many countries.So the price of the fish is very attractive to cultrist.But,the severe disease,prevalent in recent
郑光明 , 马丽莎 , 朱新平 , 刘毅辉 , 陈永乐 , 罗建仁
2008, 27(4).
Abstract:Genetic diversity between two populations of big-headed turtle was estimated using random-amplified polymorphic DNA(RAPD) assays.49 individuals were amplified using 25 arbitrary primers.Averaged 209 and 8 DNA fragments were produced in each individual and
颜福花 , 刘先葆 , 徐象华 , 李作洲 , 郁香荷 , 吴连海 , 蔡礼鸿
2008, 27(4).
Abstract:Allozymic genetic diversity of six taxa of Prunus L.,together with its two close species(Padus racemosa and Armeniaca sp.) was investigated by using vertical slab polyacrylamide gel electrophoresis.A total of 72 alleles were detected at sixteen loci of th
2008, 27(4).
Abstract:Contents of mineral element,sugar and organic acids contained in 6 Valencia orange cultivars growing in Zigui area of China were analyzed.Results showed that the fruits were better to be freshly consumed because of their relatively lower ratio of S/A.Ther
2008, 27(4).
Abstract:Based on analyzing the different areas economic development level in Hubei Province,this paper studied the economic value of forest ecological resources,the results showed that the economic value of forest ecological resources were influenced by the socie
ZHANG Rui-xia ZHANG Juan XIONG Shan-bai TAN Yan-jun
2008, 27(4).
Abstract:通过测定3.5℃、6.5℃、9.5℃和20.0℃4种水温下鲫的存活率、血液生化指标、排泄物氨态氮含量以及鱼体肌肉质构、色度和理化指标的变化,观察低温处理对鲫生化特性及肉质的影响.结果表明,鲫的氨氮排泄量随温度升高而增加,且20.0℃的氨氮排泄量显著高于其它3种温度的.在4种水温下暂养5 d后,鲫的存活率分别为85.0%、100.0%、94.7%和75.0%,在6.5℃水温下暂养保活效果最好.20℃暂养5 d后,鲫血液的乳酸脱氢酶、谷草转氨酶、血糖和皮质醇均高于低温试验组的鲫.低温处理对鱼肉的弹性和粗蛋白无影响,而对鱼肉的持水力、粘聚性、回弹性、pH值以及可溶性蛋白、粗脂肪含量有显著影响.低温处理对鲫的存活率、生化特性和肉质有明显影响,选择6.5℃的水温有利于其保活贮运.
2008, 27(4).
Abstract:In the process of normal pressure and high pressure microwave-assisted extraction,the chemical composition changes of the flavonoids from tartary buckwheat bran were studied by HPLC-UV and HPLC-ESI-MSn.The influences of pressure and microwave-assisted ext
XIA Jun-fang ZHOU Yong ZHANG Ping-hua
2008, 27(4).
Abstract:In the paper,the precise seed-meter virtual instrumentation(VI) testing system has been designed that according to the VI design idea and by using electro-optical sensor and VI based on combined software and hardware,gaving priority to software.The seed-m
2008, 27(4).
Abstract:This paper aims to study the issue of the inventory control for perishable agricultural products under inflation and delay in payment by applying the method of control theory and system optimization.The inventory control model under market demand determin
2008, 27(4).
Abstract:This paper develops an environmental surveying system based on MSP430 single chip microcontroller and nRF401 wireless communication chip.Especially this paper illustrates how to make use of the advantage of ultra-low power and high integration in MSP430 t
CHEN Xiao-qin JIANG Shi-hong LI Guang-jing
2008, 27(4).
Abstract:With the development of biotechnology,molecular biologic techniques were used extensively in insects taxonomy in recent years.In this paper,the application of DNA sequencing,RFLP,RAPD,nucleic acid hybridization,isoenzyme electrophoresis,SSCP and DSCP tech
2008, 27(4).
Abstract:Seeds of most plant species store triacylglycerols as food reserves for germination and post-germinative growth.The triacylglycerols are present in small,discrete subcellular spherical oil bodies.Oil bodies consist of a matrix of triacylglycerols surround
2008, 27(4).
Abstract:The decaffeinated tea are a kind of deep processing tea products,which contain a low-level of caffeine.In the recent 20 years,the technique of manufacturing the decaffeinated tea was profoundly and systemic studied abroad and home,and the results revealed
2008, 27(4).
Abstract:This examination system adopts automatically full textmatching searching technology and is able to solve automatically problems such as incorrect spelling wordiness and word missing.It can skip over the non-matching words and grade the paper according to
石磊 , 龚瑞 , 尹争艳 , 周勇 , 裴洁 , 胡智斌 , 王利霞 , 胡长敏 , 刘涛 , 陈颖钰 , 廖娟红 , 赵俊龙 , 陈焕春 , 郭爱珍
2008, 27(4).
Abstract:自2008年以来,湖北省多个地区肉牛养殖场(户)发生以肺肉变及坏死为特征的呼吸道传染病,病牛咳嗽,消瘦,有的继发关节炎.常规抗生素治疗效果差,犊牛及体弱牛病死率高.为此,本研究组采集病牛样本(血液和肺等内脏器官),对该病开展了流行病学与病原学研究.实验室检测项目包括:1)支原体培养鉴定,PCR扩增与基因测序鉴定;2)细菌分离鉴定;3)牛结核诊断,包括牛结核菌素皮内变态反应、牛结核菌抗酸染色、牛结核菌培养鉴定,与牛血清结核抗体ELISA检测;4)泰勒虫血涂片检测与PCR检测等.
2008, 27(4).
Abstract:Four hundred mutants were obtained from Mesorhizobium huakuii 7653R using Tn5-sacB insertion mutagenesis.Symbiotic mutant HK56 was screened by plant nodulation experiments.The DNA sequence of exo56 was determined by thermal asymmetric interlaced PCR(TAIL-
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