LV Hai-ying , YANG Qi-wen , YIN Hai-cheng , SONG Pin-ga , ZHANG Xun-pu
2008, 27(3).
Abstract:斑马鱼(Danio rerio)eomesodermin(简称eomes)是母源性基因,编码一个包含T-box的转录因子.
CHEN Guo-ju ZHAO Shuang LEI Jian-jun CAO Bi-hao ZENG Guo-ping
2008, 27(3).
Abstract:According to the DNA sequence of PamPAP in Phytolacca americana,primers were designed to amplify a non-virulent defective PAP gene(PacPAP) that only inhibit virus but not interfere host organism,from genomic DNA of leaves in Phytolacca acinos.The expressi
TANG Mi , BIE Zhi-long , ZHANG Bao-cai , WU Ming-zhu , YI Hong-ping , FENG Jiong-xin
2008, 27(3).
Abstract:The leaf photosynthetic characteristics and fruit qualities of Flavor No.3,Xueli and Xuelihong melon were investigated.The results showed that Net photosynthetic rate and leaf colour value of Flavor No.3 leaf were significantly higher than those of Xueli
2008, 27(3).
Abstract:Young leaves,and calli were excised as material,and the effect of different enzyme combination,time of enzymolysis,osmotic pressure on protoplast isolation were studied.The results are as follow:Leaves which have grown for 5~10 days are cut up,then were p
LIU Jian-li , WANG Yan-hui , GUAN Wei , XIONG Wei , YU Peng-tao , CHENG Li-li
2008, 27(3).
Abstract:2006年5~10月在宁夏六盘山北侧叠叠沟林场,运用德国Ecomatik公司生产的SF-L树干液流仪研究了9株华北落叶松的树干液流变化过程及其对环境因子(气象条件、土壤水分)的响应.结果表明:华北落叶松的树干液流昼夜变化明显,呈不太典型双峰型曲线,晴天变化幅度大(0.087~0.348cm/min),阴天变化幅度小(0.087~0.281cm/min).树干液流速率在生长季内呈现逐渐降低的趋势.对环境因子影响树干液流速率的偏相关分析表明:日液流速率(SFV)与太阳辐射(ESR)、土壤水分(Ps)各影响因子均存在显著正相关,而与温度因子(Tamean)的相关系数较低.经回归分析,建立了日液流速率与各环境因子的线性回归模型:SFV=0.106 0.000141Ps 0.00214ESR 0.00218Tamcan-00043P(R=0.883).
CHEN Yu-qiong , ZENG Wei-chao , MENG Yan , XIANG Ban-gui
2008, 27(3).
Abstract:The changes of the main characters during Tengcha processing by using a traditional and a new way were studied systematically.The result showed that the contents of flavones,proteins and chlorophylls reduced gradually in the processing,while the contents
DAI Hong-fen , LAI Zhi-yong , LI Jian-guang , HUANG Bing-xiong , XIAO Wei-qiang , WANG Xiao-rong
2008, 27(3).
Abstract:应用高效液相色谱(HPLC)法测定了黄皮、杨梅果肉中的绿原酸、表儿茶素和芦丁的含量.色谱柱为Nucleosil C18反相柱(250mm×4mm),流动相为20%的甲醇(A),30%乙腈(B),pH3.2,梯度洗脱,流速0.8mL/min,检测波长280nm,柱温35℃.结果表明:3种标准品的线性范围为0.3906~50.0000μg/mL,相关系数r=0.9999.标准品在0~48h内经多次测定,保留时间和峰面积的相对标准偏差分别为0.78%~0.91%和0.67%~0.95%;回收率和回收率相对标准偏差分别为93.21%~103.50%和2.77%~3.94%;仪器最低检出限分别为0.0901、0.0955、0.0980μg/mL.测定结果还表明:黄皮和杨梅果肉中均含有一定量的绿原酸、表儿茶素和芦丁.黄皮的4个品种中,3种物质的含量(鲜重)分别为4.0468~38.6690、4.3164~84.6148、52.3924~130.6097、μg/g;杨梅的4个品种含量(鲜重)分别为17.5644~93.5463、113.0908~236.401 5、46.3667~168.3700μg/g.
2008, 27(3).
Abstract:以明胶与卡拉胶为模式体系,研究其共凝胶体的力学特性,从而探讨其交互作用的机理.通过动态粘弹性的研究表明,所有配比凝胶体贮能模量G'都大于耗能模量G",说明共凝胶体的弹性性质高于粘性,而且随振荡频率的增加,G'和G"的值都在增加.卡拉胶浓度达到1%时,其G'都远远大于其它配比的,说明在此配比下,明胶与卡拉胶一起形成了较强的凝胶网络,凝胶体的弹性性质增加.单纯明胶其熔点在32.5℃,而添加0.5%卡拉胶后,其熔点增加至35℃,当添加1.0%后,其熔点已增加至40℃.明胶与卡拉胶形成的共凝胶体的粘弹特性随2种大分子的配比及盐浓度的不同而不同.
HUANG Hai-dong LI Ji-bo LIAO Qing-xi
2008, 27(3).
Abstract:In this paper,the shearing characteristics and parameters of the bottom stalk of ramie Boehmeria nivea(L.) Gaud Huazhu 4 in harvesting period have been studied experimentally.The study shows that the mean maximum resistance intensity of shearing is 64.1 M
CUI Ru-qiang , LIAO Jin-ling , ZHUO Kan , ZHONG Guo-qiang
2008, 27(3).
Abstract:Plant parasitic nematodes is a group of pathogens which domajor harm to crops.The pragress of the gene-related resarches on the development of plant-parasitic nematodes was briefly reviewed here.It could be concluded that few resarches on the molecular me
CAO Yan-jun , HE Li-xia , LIU Peng-cheng , ZHOU De-yi
2008, 27(3).
Abstract:With energy price rising,there are two different points on whether it takes rapeseed as raw material to develop biodiesel in China.Supporters consider that rapeseed can be planted on winter-free farmland,while opponents deem that developing rapeseed biodi
FANG Dong-quan , YANG Kui , AN Ping , HUANG Zhao-xing , XIONG Xiao-jin
2008, 27(3).
Abstract:根据华中农业大学的信息化发展现状,设计与实现了基于数字校园三大平台的图书馆门户和ILASⅡ(图书馆自动化集系统Ⅱ)集成,并初步探讨了有关图书馆服务与校园一卡通的整合问题.
2008, 27(3).
Abstract:According to the status of the development of informationization of Huazhong Agricultural University,the library portal integration and ILASII integration based on the three main platforms in digital campus are designed and realized.Integration of library
MA Kang ZHOU Jie QIN Rui LIU Xin-qiong LIU Xue-qun WANG Chun-tai
2008, 27(3).
Abstract:According to the sequence from the rice date base,8 primer pairs for 8 VDAC genes were designed.The fragments of 8 VDAC genes were amplified with the template DNA of different wild rice(with BB,CC,BBCC and CCDD genome) and cultivated rice(AA genome) throu
QIAO Yan-yan , YANG Cui-yun , YU Cui , CAO Jie , MA Qing , ZHANG Li-li
2008, 27(3).
Abstract:The CP fragment of the Tobacco ringspot virus(TRSV) was amplified by RT-PCR,and inserted into the expression vector pET28a.The recombinant plasmid pET-CP was transformed into Escherichia coli BL21(DE3),the target protein was expressed with induction of IP
ZENG Cui-ping WANG Xiao-du DAI Han-chuan LONG Liang-qi WU Xiao-xiong
2008, 27(3).
Abstract:将猪IFN-α(poIFN-α)成熟蛋白基因克隆于pET-28a载体中,转染大肠杆菌,并进行诱导表达.经SDS-PAGE检测,融合表达的蛋白poIFN-α大小约27ku.收获融合表达的poIFN-α,免疫BALB/c小鼠,取脾细胞与SP2/0骨髓瘤细胞进行融合,通过间接ELISA方法对融合细胞进行检测,结果获得了1株能稳定分泌抗poIFN-α蛋白抗体的杂交瘤细胞株,命名为2H12株.Western-blotting结果及单克隆抗体亚型鉴定结果表明,单抗与重组蛋白发生特异性反应,单抗为IgG1亚型,且轻链为κ链.
ZHANG Yu-shan , WU Wei , WANG Jian-bo , XU Cai-guo
2008, 27(3).
Abstract:In order to explore the reasons of yield formation of extreme lines of high yield from the point of view of source and sink,recombinant inbred lines(RILs) population(n =190) was constructed by single-seed descendent from a cross between Zhenshan 97 and HR
DENG Wei-an , ZHENG Zhe-min , WEI Shi-zhen
2008, 27(3).
Abstract:A new species of the genus Mazarredia Bolivar is described.Type specimens are deposited in the Institute of Zoology,Shaanxi Normal University,China.Mazarredia puzheheiensis sp.nov.(Fig.1)The new species is allied to Mazarredia huanjiangensis Zheng et Jian
ZHOU Yin-li , HU Xian-qi , GUO Ya-li , ZHANG Guo-wei , LIN Li-fei
2008, 27(3).
Abstract:During 2003~2006,the investigation of megranate parasitic nematodes has been conducted,the soil samples were collected from Mengzi megranate garden,Mengzi County and Huize megranate garden,Huize County as well as Jianshui megranate garden,Jianshui County
YOU Chun-ping LIU Ren XIAO Ai-ping LIU Kai-qi LIANG Yi CHEN Yu-xiu
2008, 27(3).
Abstract:将3株香蕉枯萎病拮抗细菌bio-d4、bio-d5和bio-B分别涂布于含有不同浓度的利福平和青霉素的培养基上,筛选出可作为抗药性标记的拮抗细菌bio-d5和bio-B.将抗药性标记的拮抗细菌bio-d5接种于香蕉植株根部,1d后再接种香蕉枯萎病菌,在不同时间测定拮抗细菌在根际、根表和根内的定殖情况.结果显示,将拮抗细菌接种香蕉根部10d后,在植株根际、根表存在少量拮抗细菌,在根内尚未发现拮抗细菌;接种后20d,在植株根际、根表定殖的拮抗细菌增多,根内也定殖了少量的拮抗细菌;在接种后30d,于根表、根际和根内定殖的拮抗细菌量达到最大;在接种后40d,于植株根际、根表和根内定殖的拮抗细菌量稍有下降.
ZHOU Xing-miao PAN Ya-fei WANG Xiao-ping LI Hong LEI Chao-liang
2008, 27(3).
Abstract:Legume pod borer Maruca vitrata(Fabricius) is one of the harmful pests of bean vegetables,such as cowpea,haricot bean and kidney bean.The survival,development and fecundity of rates legume pod borer fed on cowpea,haricot bean and kidney bean were measured
TIAN Yong-qing ZHUANG Liu-dong XU Han-hong
2008, 27(3).
Abstract:Diphenylcarbazide was applied to react with 50 plant extracts respectively,and color change could be seen if the extract contained photoxins when the reaction was in the presence of ultraviolet.According to the fact that diphenylcarbazide could be oxidize
WEN Li-hua , SHI Zhi-hua , WANG Tian-wei , CAI Chong-fa , LIU Hong-yao
2008, 27(3).
Abstract:The land use changes in Xianning City,Hubei Provine using the MSS image in 1978,the TM image in 1991 and the ETM image in 2002 were demonstrated.The result indicated that from 1978 to 2002 the forestland was major element in the area studied.The forestlan
LIU Bi-rong ZENG Chang-di XIAO Hong-dong WANG Hui-zhen FANG Ming-hui YU Min
2008, 27(3).
Abstract:Influences of boron on the activity of superoxide dismutase(SOD),production rate of superoxide anion radical and the content of malondialdehyde(MDA)in Seashore paspalum and Tifdwarf were studied under media culture in spring and early autumn for understan
LIU Jin-fa XIA Ren-xue WANG Ming-yuan RAN Qing-qing WANG Peng LUO Yuan
2008, 27(3).
Abstract:The effect of arbuscular mycorrhizal(AM) fungi on utilization of insoluble phosphate and the growth in Poncirus trifoliate(L.) Raf.seedlings was studied in green house.The seedlings were grown in the sterilized sand amended with Al-P or Fe-P,inoculated wi
WEI Tian-tian , YE Tian-tian , CHENG Guo-jun , LI You-guo
2008, 27(3).
Abstract:从湖北省某重金属矿区的土壤中分离、筛选到1株能够还原Cu2 的菌株TT1.该菌株可以单抗3mmol/L的Cu2 .经对该菌株进行形态特征以及16SrDNA序列比对分析,确认该菌株属于地衣芽孢杆菌(Bacillus licheniformis).TT1与Bacillus licheni formis的同源性最高,达到98%.在含有0.2mmol/L Cu2 的培养条件下,菌株TT1的生长不受影响.Cu2 还原的最适温度为30℃,在该温度下,24h时间内TT1可以还原71%的Cu2 ;Cu2 最适还原的pH为6.因此,TT1菌株有望用于含Cu2 废水的处理.
ZENG Qing-lan , HONG Yu-zhi , LIU Zi-duo
2008, 27(3).
Abstract:以稻秆作为唯一碳源,研究了氮源、接种量、培养时间、培养温度、培养基初始pH等对丝状真菌菌株Gibberella fujikuroi产纤维素酶的影响.结果表明:该丝状真菌菌株产纤维素酶的最适氮源为CO(NH2)2,接种量为5%,培养时间为120h,培养温度为28~37℃,培养基初始pH为5~6.在最适宜的条件下,培养液中内切葡聚糖酶活力、天然纤维素酶活力和滤纸酶活力分别可达到1.723IU/mL、0.368IU/mL和0.344IU/mL.
QIAO Xi-nan , YANG Guo-yu , ZHU Yan-cai , WANG Yue-ying , HAN Li-qiang , WANG Yan-ling
2008, 27(3).
Abstract:A pair of cloning primers were designed based on the in silico sequence information.Total RNA was extracted from mucosal tissue of ovine ileum and mRNA sequence was amplified by RT-PCR.The PCR products were ligated into the pMD19-T vector,and then transfo
QIU Hai-fang LIU Bang DONG Xin-jie ZHANG Qing-de
2008, 27(3).
Abstract:通城猪是湖北省的主要地方品种,具有产仔多、性成熟早、发情明显、配种容易、母性好、抗逆性强、遗传性稳定、杂交配合力好等优良特性,尤以肉嫩清香鲜美著称,2000年被列为19个国家级优良地方猪保护品种之[1-2].
DONG Jun-feng FAN Qi-xue ZHANG Lei LI Bo YANG Kai FANG Wei
2008, 27(3).
Abstract:将来自于同一亲本的黄颡鱼按规格分为A、B、C 3组,A组为未分级组[体长(5.32±0.52)cm,体重(1.60±0.54)g],B、C 2组为分级组,其中B组为大规格组[体长(5.63±0.41)cm,体重(1.88±0.50)g],C组为小规格组[体长(4.85±0.22)cm,体重(1.11±0.17)g],饲养在9个网箱中.经60d养殖,B组增长率显著高于C组,A、B 2组无显著差异;3组增重率无显著差异.A组的体长和体重的变异系数显著高于B、C 2组,B、C 2组之间无显著差异;C组的体长和体重变异系数的变化率显著高于A、B 2组.各组的成活率、特定生长率、雌雄比率,成熟度指标均差异不显著;各组中雄性的增长率、增重牢、特定生长率均高于雌性,除A组增长率雌雄差异不显著外,其余各组各指标雌雄差异均显著;而成熟度各组中雌性均显著高于雄性.试验结果表明,黄颡鱼早期分级养殖,有利于保持整体规格的一致,但对整体的成活率及特定生长率等无显著影响.
SHI Chun-lu LIU Xiao-ling GE Hai-yan
2008, 27(3).
Abstract:炎症是免疫系统对感染的重要应答.以皮质醇混饲投喂的方法,模拟自然发生的应急反应,研究了应激激素皮质醇对黄颡鱼腹腔渗出炎症细胞的影响.结果表明:通过腹腔注射液体石蜡后诱导的黄颡鱼腹腔渗出炎症细胞主要包括嗜中粒细胞、巨噬细胞和嗜酸性颗粒细胞(EGC).注射后2d,腹腔渗出细胞以嗜中性粒细胞为主(占嗜中粒细胞和巨噬细胞总数的61.5%±1.1%);注射后6d、10d则以巨噬细胞为主(在嗜中粒细胞、巨噬细胞和EGC分类计数中分别占77.8%±2.8%和82.3%±1.1%);注射后6d出现EGC,随后数量逐渐增加.炎症过程中腹腔渗出嗜中粒细胞和巨噬细胞对福尔马林灭活金黄色葡萄球菌(F-SA)的吞噬率和硝基四氮唑蓝(NBT)反应阳性细胞率逐渐上升.皮质醇混饲投喂使腹腔渗出巨噬细胞比例和吞噬细胞的吞噬率在注射液体石蜡后6d时显著下降,而EGC比例显著上升.
JIANG Fang-jun , GAO Long-ying , HE Jun-qiang , JIANG Yu-lin
2008, 27(3).
Abstract:Sick grouper fry in Fujian Province were collected.Vacuolation was obviously observed in brain and retina of diseased fry in histopathological section.The fry organs were homogenized and sup-ernatant was inoculated to SSN-1 cell.CPE was developed on the 2
TIAN Fei-yan , LV Jian-qiang , LIU Hong , LI Hui-fang , LIU Zong-xiao , CHEN Chang-fu
2008, 27(3).
Abstract:根据已报道的鱼类病毒性神经坏死病毒(viral nervous necrosis virus, VNNV)衣壳蛋白基因(coat protein gene, cp基因)设计引物,对2006年从我国沿海养殖石斑鱼中分离到的6株鱼类神经坏死病毒进行RTPCR扩增,特异性扩增出6株病毒的完整衣壳蛋白基因,分别连接到pMD18-T载体中,并进行序列测定和分析.结果发现,6个VNNV分离株的cp基因核苷酸序列的同源性在97.3%以上;推导的氨基酸序列同源性在79.6%~99.1%之间.根据cp基因核苷酸序列绘制分子进化树,发现6个VNNV分离株在进化树同一分枝上,属于RGNNV基因型,与已报道的点带石斑鱼神经坏死病病毒(ECNNV)、巨石斑鱼神经坏死病病毒(ETNNV)及玛拉巴石斑鱼神经坏死病病毒(MGNNV)亲缘关系较近.试验结果表明,目前我国沿海养殖场发生的鱼类病毒性神经坏死病均由同一来源的病毒引起,该基因型的鱼类神经坏死病毒在我国沿海养殖的石斑鱼中广泛传播流行.
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