2007, 26(3).
Abstract:Scientific viewpoint of development is systematic development theory,which is people-oriented and balanced.Sustainable development is the basic principle for our country's developmental strategy in new era.Eco-city construction reflects the significant ch
2007, 26(3).
Abstract:Based on the research of traditional Chinese temple garden,the spatial distribution has carried out some analysis on the entirety of Wuhan Buddhist Temple garden space,and tier of space artistic conception has been listed.In summing up the open and closed
2007, 26(3).
Abstract:以猕猴桃果实为原料提取并分离纯化,得到碱溶性多糖组分,通过采用正交试验得到提取碱溶性多糖的最佳方案。该多糖经层析方法和Smith降解等反应,证实其为单一组分。该组分多糖由葡萄糖为单一糖基组成,并证实该多糖具有β(1→3)及β(l→6)连接的糖苷键。
LIAO Qing-xi , SHU Cai-xia , TIAN Bo-ping , HUANG Hai-dong , WANG Jing , LIAO Yi-tao , WU Fu-tong
2007, 26(3).
Abstract:The Arundo donax L.is a kind of good material of paper making,which is tall,thick and hard.In order to analyze the cutting process of the A.donax,a cutting experimental system of A.donax harvester based on the high-speed photography technology including t
XIA Jun-fang , XU Chang-yu , ZHOU Yong
2007, 26(3).
Abstract:以2BJD-4大豆精密播种机配套排种器为研究对象,基于ADAMS动态仿真分析软件,建立了补偿系统的虚拟样机模型,找出了适合补种装置的结构形式和影响性能的结构参数,进行了种子运动轨迹动态模拟和最优化仿真,确定了影响补种质量的3大因素,即:弹簧刚度系数K、电磁铁吸力F和型孔斜度θ,并在给定的条件下确定其最优参数值分别为1.5102 N/mm,49.849 N,-26.52°。根据最优虚拟样机模型试制出物理样机模型并进行试验,结果验证了仿真优化结果的正确性和可靠性。
JIANG Lin , ZHANG Dan , LIU Yu-rong , LIANG Yong , ZHANG Jie
2007, 26(3).
Abstract:The root tips of Vicia faba were treated with the hospital filthy water,industrial wastewater and domestic sewage from South Lake.Through normal squashed specimen,the effect on mitosis process of the root tip cells of Vicia faba was investigated.The resul
2007, 26(3).
Abstract:Fast-growing rhizobia often contain one or more large indigenous plasmids.One of these plasmids,called pSym,carries the nodulation and nitrogen fixation genes necessary for establishing the Rhizobium-legume symbiosis;the other plasmids called non-pSym.In
LI Jing , LIU Xue-qun , WANG Chun-tai
2007, 26(3).
Abstract:利用笔者所在的实验室从烟草中克隆的一个葡糖基转移酶基因(GT-like),通过PCR扩增得到该基因开放阅读框5′端-1150~0上游调控区。序列分析表明该启动子序列含有多个基因表达调控元件。将GT-like基因启动子与gus报告基因连接,构建植物表达载体pGT-gus,经根癌农杆菌介导法转化W38型烟草。转基因烟草植株GUS组织化学染色结果表明:该基因上游-1150~0序列具有启动子活性,能启动gus基因在烟草叶片中表达,而在根中的表达受时间和环境影响。GUS诱导活性的定量分析结果表明,该启动子的表达不但受甲基茉莉酸的强烈诱导,而且也受水杨酸的强烈诱导。对这一启动子的诱导表达机理的分析将有助于进一步研究甲基茉莉酸和水杨酸之间拮抗作用的相互关系。
2007, 26(3).
Abstract:A systematic cytological comparison of the anther development of the PSGMS rice with its normal fertility counterpart was conducted.The results showed that pollen abortion in the PSGMS rice occurred first no later than the pollen mother cell(PMC) stage an
HE Yun-wei , HONG Ni , XU Wen-xing , WANG Guo-ping
2007, 26(3).
Abstract:Citrus exocortis viroid(CEVd) in Citrus sinensis Osbeck cv.Robertson,which showed typical symptoms of CEVd infection,were detected by bi-directional PAGE electrophoresis,RT-PCR,RNA dot-blotting and tissue-printing hybridization.Results showed that RT-PCR
2007, 26(3).
Abstract:The genetic transformation system of somatic embryos of papaya(Carica papaya L.) cultivar Meizhonghong mediated by Agrobacterium tumefaciens using genes from Papaya ringspot virus(PRSV) was established.The results showed that the concentration of Agrobact
RYU K Y , YANG Yan-li , HU Xian-qi , LU Shao-feng , LUO Wen-fu , RYU K Y , XIAO Lang-tao
2007, 26(3).
Abstract:117 isolates came from potato plant of 26 collection areas in 10 counties and cities in Yunnan Province were tested with 11 mono-gene R1~R11 potato clones of potato late blight differential host.The results showed that there are 26 race in Yunnan Province
2007, 26(3).
Abstract:Based on field investigation,pathogen identification,artifical inoculation and examination of lily diseases in Western Henan Province,13 lily diseases were found including 6 species of fungic diseases,3 species of bacterial diseases,3 species of virus dis
CHENG Dong-mei , ZHANG Zhi-xiang , HU Mei-ying
2007, 26(3).
Abstract:测定了闹羊花素-Ⅲ对斜纹夜蛾4龄幼虫的拒食、触杀和胃毒作用。结果表明:闹羊花素-Ⅲ的杀虫方式主要以拒食和胃毒作用为主,触杀作用较弱;闹羊花素-Ⅲ处理斜纹夜蛾4龄幼虫24 h后,叶碟法测得的AFC50为16.63μg/mL,以叶片夹毒法测得的LC50为66.56μg/mL,以点滴法测得LD50为每头30.72μg,Potter喷雾法测得的LC50为590.35μg/mL。观察结果还表明,闹羊花素-Ⅲ能显著降低斜纹夜蛾幼虫的取食量、幼虫总糖量和血淋巴总糖含量。
ZHANG Zhi-xiang , CHENG Dong-mei , XU Han-hong , TIAN Yong-qing
2007, 26(3).
Abstract:室内试验测定结果表明,印楝种子甲醇抽提物中印楝素B在甲醇、乙醇、乙腈和DMF等溶剂中具有良好的稳定性。在54℃下贮存14 d,印楝素B的降解率分别为-1.87%、1.13%、-2.32%和5.24%。印楝素干粉中印楝素B在甲醇、乙醇、丙酮和DMF等溶剂中的稳定性不佳,在54℃下贮存14 d,印楝素B的降解率分别为24.07%、35.65%、19.88%和10.29%。印楝种子甲醇抽提物在甲醇 水(55/3,V/V)中于0℃下贮存7 d和14 d,印楝素B的降解率分别为-2.98%和1.03%。于54℃下贮存7 d和14 d,印楝素B的降解率分别8.73%和15.09%。于甲醇 水(9/1,V/V)中在54℃下热贮14 d,印楝素B的降解率为52.47%,稳定性高于印楝素A。紫外光下照射19 d,印楝种子甲醇抽提物中印楝素B的降解率为-2.94%。
HUANG Ji-guang , XU Han-hong , ZHOU Li-juan , YANG Xiao-yun , ZHANG Ting-ying
2007, 26(3).
Abstract:The seasonal variation of rotenone content in Tephrosia vogelii Hook f.leaves of purple and white varieties importend from Philippines and Tanzania were detected with Sohxlet and HPLC.Results showed that the content of rotenone in T.vogelii leaves was dif
YANG Chang-long , JIANG Shi-hong , XU Han-hong
2007, 26(3).
Abstract:The oviposition repellent effect of alcohol extracts from twelve plants on Conopomorpha sinensis Bradley was investigated.The result showed that the oviposition repellent effect of alcohol extracts of Bauhinia blaakeana was the best.The choice and non-cho
SONG Yue-qin , LI Yi-ping , SUN Hui-zhong , WU Jun-xiang , HE Cheng-yi
2007, 26(3).
Abstract:斜纹夜蛾(Spodoptera literaFabricius)是一种世界性害虫,是我国农作物上常发生暴食性害虫之一,可危害99科,290种植物。近年来,由于种植结构等因素,斜纹夜蛾在我国发生危害的地区逐渐扩大,已成为农作物的重要害虫[1-2]。关于斜纹夜蛾的研究报道颇多[3-5],但未见关于气门扫描电子显微镜下的超微结构方面的报道。气门(spiracle)是昆虫气管在体壁上的开口,是外界空气进入体内的第1道结构单位[6-7]。昆虫气门和其他组织结构一样,也是昆虫长期的自然进化进程中与生境协同适应的结果,了解气门形态分类特征便于更深入的掌握和解释昆虫的生物学特性…
ZHOU Ming-hua , HUANG Guan-yi , CHEN Yi-ping , NI De-zhi , HU Hong-qing
2007, 26(3).
Abstract:An investigation on temporal and spatial distribution of N in marsh soils of pond-wetlands system in the artificial wetland was conducted in a little experimental watershed of Wuhan Zoo,Hubei Prorince.Results showed that the contents of total nitrogen(TN)
LIU Yan , HOU Long-yu , CUI Xiao-dong , MA Feng-yun , XING Shang-jun
2007, 26(3).
Abstract:The relationships between soil urease activity and soil nutrient contents of four different wetlands in Yellow River Delta were studied through path analysis.The results showed that the standard multiple regression equation by the set of independent varia
ZENG Xian-lu , LIANG Ji-nan , TAN Zhong-wen
2007, 26(3).
Abstract:Effects of silicate on some photosynthetic characteristics of sugarcane leaves were studied.The upper and lower leaves were compared,including leaf water content,water potential,leaf erectness,chlorophyll content,activity of nitrate reductase,leaf area in
DING Yan-rui , CAI Yu-jie , SUN Jun , RAO Zhi-ming , XU Wen-bo
2007, 26(3).
Abstract:All complete genome unicellular microorganisms were selected from NCBI COG database and all proteins whose three-dimensional structure had been measured were collected to study the influence of salt bridge on protein thermostability(the proteins had been
WANG Jian-ping , WANG Xiao-li , WANG Chang-jun , JI Zhi-xia , CHEN Shou-wen , YU Zi-niu
2007, 26(3).
Abstract:用不同浓度聚-γ-谷氨酸(γ-PGA)溶液对烟草种子进行不同时间浸种处理,通过种子发芽率、种子活力与种子酶活性的变化,筛选出γ-PGA浸种的最佳浓度和时间;还研究了不同浓度γ-PGA溶液灌根对烟草苗期生长的影响。结果表明,用0.10~0.30 g/L的γ-PGA溶液浸种1~3 d可以提高烟草种子的发芽率、种子活力,缩短出苗时间。处理后的种子淀粉酶、过氧化物酶和过氧化氢酶的活性与对照相比均有不同程度的提高,以0.20g/L的γ-PGA溶液处理种子1 d效果最好。灌根试验表明,烟草苗期生长的最适γ-PGA浓度也为0.20 g/L。
JIN Shuang-xing , ZHANG Gui-zhi , LI Yue-min
2007, 26(3).
Abstract:To investigate the effect of different basic mediam and methods on the in vitro culture of porcine preantral follicles,two basic medium of M199 and NCSU23 and three of 96-well plates,hollowness and granulose cells coating culture systems were adopted in c
SHEN Zhong-yan , ZHAO Shu-miao , LIANG Yun-xiang
2007, 26(3).
Abstract:The lactic acid bacteria(LAB) in this experiment were isolated from the faeces of local healthy pigs.Three of them were most tolerant to in vitro antagonism of enteric pathogenic microorganisms.They were identified as Enterococcus faecium,Enterococcus fae
ZHONG Kai , YANG Guo-yu , LIU Yi , WANG Yan-ling , ZOU Si-xiang , CHEN Wei-hua
2007, 26(3).
Abstract:选择同处于泌乳初期的6头睢宁白山羊为试验动物,于左、右乳区分别灌注黄芪多糖和生理盐水5 mL,连续灌注5 d,第6天注入大肠杆菌内毒素诱导产生山羊实验性乳腺炎。于注入内毒素前1 h及灌注后3、6、9、24 h取颈静脉血和采集乳样,测定乳液及血清中N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活力及丙二醛含量(MDA)等指标。结果表明:与灌注内毒素前相比,灌注生理盐水的对照乳区乳中的NAGase活力在注入内毒素后9 h时达到峰值(23.95 U/mg),而灌注黄芪多糖的试验乳区乳中的NAGase活力6 h时达到峰值(28.29 U/mg),24 h时乳中NAGase活力已下降到灌注内毒素以前的水平。山羊乳导管注入内毒素后,2种处理的乳区乳中的总抗氧化能力显著下降,乳中MDA的含量显著升高,SOD活力显著下降;2种处理的乳区乳之间比较无显著差异。
FU Yuan , ZHANG Xue-juan , ZHAO Jun-long , HE Yong-qiang , LIU En-yong , LU Fu-zhuang , JIANG Tao , FENG Shang-lian
2007, 26(3).
Abstract:In order to get the recombinant Schistosoma japonicum 22.6 ku tegumental protein,a pair of primers was designed.The 576 bp fragment was cloned by using reverse transcirption polymerase chain reaction(RT-PCR) from the mRNA of Schistosoma japonicum.Sequence
ZHAO Fei , WU Zhi-xin , PANG Su-feng , PENG Jian , NIE Pin , CHEN Xiao-xuan
2007, 26(3).
Abstract:试验鱼以投喂饲料的不同和是否注射抗原共分为10组,即免疫组:A、B、C、D、E组,免疫对照组:a、b、c、d、e组,饲料对照组:A、a组.饲料试验组B、C、D、b、c、d、e组.其中,饲料对照组以豆粕和鱼粉为基础蛋白源,饲料试验组分别以双低菜籽粕和普通菜籽粕等氮替代对照组中50%(B、b;D、d)和100%(C、c;E、e)的豆粕蛋白,测定异育银鲫血液白细胞和头肾吞噬细胞的吞噬活性、血清溶菌酶活性、血清补体(C3,C4)含量、血清凝集抗体效价及免疫保护率.结果表明:从免疫后21 d开始,饲料试验组E、e、C、c组的各项免疫指标都显著低于相应饲料对照组;在49 d,D、d组的部分免疫指标也显著低于相应饲料对照组;B、b组和相应饲料对照组之间没有显著的变化.免疫组的各项免疫指标均显著高于相对应的投喂相同饲料的免疫对照组.
LIU Wen-kui , FAN Qi-xue , ZHU Bang-ke , DU Hai-ming
2007, 26(3).
Abstract:研究了饵料密度(每毫升0.1个、0.3个、1.0个、3.0个和10.0个)对3种规格(I:0.68 cm;II:1.50 cm;III:3.20 cm)杂交鳢仔鱼生长、存活的影响。结果表明:在饵料密度为每毫升0.1~1.0个的范围内,3种规格仔鱼的特定生长率随饵料密度增加而显著升高,当饵料密度为每毫升1.0个时,特定生长率达到最高值,随着饵料密度进一步增加,规格I仔鱼的特定生长率随饵料密度的增加而下降,规格ⅡI、II仔鱼的特定生长率无显著变化。饵料密度对成活率没有显著影响,各饵料密度组的成活率都很高。规格差异对饵料密度不敏感,3种规格仔鱼的最适饵料密度均为每毫升1.0个。
XIA Lu , WANG Cheng-zhu , CHEN Chang-fu
2007, 26(3).
Abstract:Channal catfish(Ictalurus punctatus Rafinsque)were immunized with outer membrane protein(OMP) extracted from Aeromonas hydrophila,Cytophaga columnaris and Edwardsiella ictaluri.The immunogenicity of OMP to channal catfish were compared by examing the aggl
ZHANG Xun-pu , CHEN Yu , GONG Shi-yuan
2007, 26(3).
Abstract:对绢丝丽蚌鳃组织的形态和显微结构进行了观察。结果表明:内外鳃瓣的鳃丝在组成上是相同的,均由鳃丝壁、肌肉组织和结缔组织构成。鳃丝上分布有丰富的前纤毛、后纤毛以及侧纤毛,内部则存在着大量鳃腺。用于连接鳃组织的丝间隔和瓣间隔,纤毛稀少。丝间隔呈“Z”字形连接。在雌蚌的内外鳃瓣之间出现了一定的结构差异,这与胚胎在雌蚌外鳃孕育有关。
2007, 26(3).
Abstract:Since 2001,20 persimmon cultivars have been introduced to Luoyang,Henan Province.Based on the evaluation of growth,prolificacy and fruit quality,adaptability etc.,5 fine cultivars of early,middle and late-maturing PCA & PCNA were screened,among the introd
DU Ke-bing , SHEN Bao-xian , XU Lin , TU Bing-kun , CAI Chong-fa , DAI Miao-hong
2007, 26(3).
Abstract:用离体培养基法研究了不同贮藏温度下杨树花粉生活力随时间变化的规律及贮藏后的花粉用于杂交育种的可行性.结果表明:Ⅰ-63杨和辽宁杨花粉于-20℃贮藏1 a后生活力分别为23.77%和34.19%,与新鲜花粉相比,其生活力分别降低48.51%和62.23%,花粉管长度分别短48.9%和48.45%.用4℃条件贮藏1 a或-20℃条件贮藏2 a的花粉授粉后可以得到正常萌发的种子,尤以-20℃下贮藏的效果为好.
SHU Chang-qing , ZHAO Xi-mei , YANG Zhen , WANG Lin , ZHANG Hai-zhong
2007, 26(3).
Abstract:A preliminary research on the cutting propagation of Ligustrum lucidum,Ligustrum quihoui and Ligustrum vicaryi was carried out.The results showed that rooting percentages of L.quihoui and L.vicaryi were high and there is no significant difference among th
ZHOU Ming-qin , YANG Qin-jun , CHEN Long-qing
2007, 26(3).
Abstract:为了建立蜡梅AFLP-银染的优化体系,采用EcoRI和MseI 2种限制性内切酶酶切组合,对该2种酶的用量、酶切时间、预扩增与选择扩增中的Mg2 浓度、Taq DNA聚合酶的用量、引物的浓度以及预扩增产物的稀释倍数等因素进行了多水平的筛选,同时探讨了温度及聚丙烯酰胺凝胶电泳(PAGE)的电流强度等因素对胶图的影响。结果表明:用5 U限制性内切酶酶切4 h便能在40μL的反应体系中完全切割500 ng的总DNA,酶切连接产物稀释10倍后在含有1.5 mmol/L Mg2 1、U Taq、预扩增引物E A和M C分别为40 ng的20μL反应体系中进行预扩增,将预扩增产物稀释30倍后在含有1.5 mmol/L Mg2 、0.6 U Taq、选择扩增引物E 3为30 ng、M 3为60 ng的20μL的体系中进行选择性扩增,能够得到质量比较好的AFLP胶图。此外,在进行PAGE电泳和硝酸银染色,将电泳的温度控制在50℃左右,电流控制在45~60 mA左右能提高胶图的质量。利用这种优化的反应体系,成功地评价了40个蜡梅基因型的遗传多样性,6对选择扩增引物组合表现出较高的稳定性、清晰度和多态性,它们分别是:E-AAC/M-CCC,E-ATC/M-CCC,E-AGA/M-CAG,E-AAA/M-CAC,E-ATA/M-CCA,E-ATT/M-CCA。
WANG Li-mian , HU Yong-hong , QIN Jun , GAO Kai , HUANG Juan
2007, 26(3).
Abstract:In order to obtain the most ecological functions of carbon fixation and oxygen production,the photosynthesis velocities of 151 plants in Shanghai were measured.On the basis of the measurement,carbon fixation and oxygen production ability was calculated.Th
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