YAN Xue-rui , CHEN Wen-feng , CHEN Wen-xin , FU Jun-fan , XUE Cai-yun , SUI Xin-hua
2007, 26(2).
Abstract:The study aimed to investigate the rhizobia isolated from Caragana microphylla growing in Liaoning Province of China.The nodules from 5 different sites were separated,purified and cultured in YMA medium.The isolates'symbiotic ability was confirmed by nodu
LI Yun-feng , CAO Yan , JI Chun-yan , WANG Zhen-zhong
2007, 26(2).
Abstract:利用PLACE数据库,对大麦β-1,3-葡聚糖酶同功酶基因PGⅢ启动子的可能顺式作用元件进行分析,并设计特异性引物。通过5′-缺失法获得了不同大小的启动子片段,分别与报告基因gus(β-葡聚糖酸醛苷酶基因)耦联,构建植物表达载体,通过农杆菌介导法转化水稻胚性愈伤组织。GUS组织化学染色结果表明,稻瘟菌来源的激发子可以诱导PGⅢ启动子不同缺失体驱动的gus表达。荧光法结果表明,缺失体P1(-1 107 bp)具有最高的激发子诱导活性,而缺失体P4(-359 bp)驱动的gus表达量迅速降低,缺失体P5的诱导活性最低。这表明转录起始位点上游-1 107 bp序列是保持启动子最高活性所必需的,而-572--359 bp的序列是保持激发子诱导活性所必需的。GCC框和GTAC序列可能是激发子诱导表达的顺式作用元件。
CHEN Xiao-qing , LUN Xuan , ZHOU Ling-yun , XU Dong-lin , ZHOU Guo-hui
2007, 26(2).
Abstract:An isolate of Sugarcane mosaic virus(SCMV),named SCMV mz-gz1,was obtained from sweet maize in Guangzhou,China.Viral CP gene sequence analysisIt was revealed that this isolate was a unique molecular variant in MZ group of SCMV.It shares 91.6% identity with
2007, 26(2).
Abstract:By using Agrobacterium-mediated transformation method,cry1Ab gene encoding Bt protein was introduced into an elite restorer line Minghui63.92 independently transformed rice plants were obtained,of which 13 had a single-copy transgene.11 Homozygous transge
LIU Wen-jun , WANG Ling-qiang , HE Yu-qing
2007, 26(2).
Abstract:Quantitative trait loci(QTL) were identified for heading date and plant height in rice(Oryza sativa L.),using two recombinant inbred line populations derived from the cross Zhenshan97/Delong 208 and the cross Zhenshan97/Nanyangzhan.Totally,11 QTLs for hea
WANG Chun-xia , LIAO Yu-cai , ZHANG Jie , LIU Zheng-wei , HUANG Tao , LI He-ping
2007, 26(2).
Abstract:To optimize the system of plantlet regeneration from wheat microspore,the effect of cold treatment and induction time on the morphology of microspore,embryogenesis and green plant regeneration from anther and young spikes of wheat was investigated.The res
2007, 26(2).
Abstract:记述采自黑龙江省蝗总科昆虫2新种,即黑翅异爪蝗Euchorthippus nigripennissp.nov.及北安拟绿洲蝗Chrysochroides beianensissp.nov.。模式标本保存于陕西师范大学动物研究所昆虫标本室。
LIU Qiong-guang , LIANG Jian-mei , LI Zhi-wei , ZENG Wei-da
2007, 26(2).
Abstract:The study was done about the identification of pathogenic bacteria causing Anthurium bacterial leaf blight which was found in Fangcun flower plantation in Guangzhou,Guangdong Province.With the researching of morphological,bacteria staining,physiological a
ZHANG Shu , ZHANG Qiu-dong , LUO Han-gang , LU Liang , YANG Xiao-lin , CHANG Xiang-qian , XIAO Zhi-min , WU Jia-tao , CHEN Qi-zhi
2007, 26(2).
Abstract:于水稻破口前3 d施用8种药剂防治稻曲病,结果表明,爱苗、万兴、好力克、井冈霉素、HB20-1、纹曲宁、三唑酮对稻曲病的防效分别达67.53%、62.15%、61.75%、57.52%、52.11%、48.35%和29.68%,其每公顷理论和实际增产幅度分别达1.71%-19.62%和3.85%-15.03%。于水稻孕穗期、破口前3 d、齐穗期、扬花期施用爱苗和井冈霉素防治稻曲病,结果表明,随着生育期推迟,对稻曲病的防治效果及产量均呈下降趋势,且以孕穗期施药防效最佳,增产最高。爱苗、井冈霉素孕穗期施药防效分别达到67.03%、58.54%,每公顷理论产量分别比不施药处理增产11.89%和10.68%,每公顷实际产量分别比不施药处理增产了11.31%和9.34%。
ZHANG Zhi-xiang , CHENG Dong-mei , XU Han-hong , WU Yu-lin , YING Biao-lin , CHEN Li , YANG Xiao-yun
2007, 26(2).
Abstract:2′,3′,22,23-四氢印楝素A和22,23-二氢印楝素A是印楝素A氢化加成产物。处理后24 h,2′,3′,22,23-四氢印楝素A、22,23-二氢印楝素A和印楝素A对斜纹夜蛾3龄幼虫的AFC50值分别为6.55μg/mL、2.89μg/mL和0.87μg/mL,处理后48 h,分别为12.61μg/mL、8.01μg/mL和1.81μg/mL。处理后24 h,3μg/mL 2′,3′,22,23-四氢印楝素A、22,23-二氢印楝素A和印楝素A对小菜蛾3龄幼虫的拒食率分别为63.74%、80.77%和85.33%;5μg/mL 2′,3′,22,23-四氢印楝素A、22,23-二氢印楝素A和印楝素A对棉铃虫3龄幼虫的拒食率分别为82.75%、90.74%和94.90%。3μg/mL 2′,3′,22,23-四氢印楝素A、22,23-二氢印楝素A和印楝素A处理后72 h,小菜蛾3龄幼虫的校正死亡率分别为77.90%、85.96%和90.42%;5μg/mL 2′,3′,22,23-四氢印楝素A、22,23-二氢印楝素A和印楝素A处理后72 h,棉铃虫3龄幼虫的校正死亡率分别为94.19%、72.86%和99.05%。2′,3′,22,23-四氢印楝素A和22,23-二氢印楝素A可明显降低棉铃虫幼虫的体重。
LIAO Zhi-wen , GONG Xi-min , HU Cheng-xiao , ZOU Juan , LI Xiao-kun
2007, 26(2).
Abstract:The soil nutrient contents in 24 soil samples which were collected from four main rice planting regionsof southeastern,middle and northern Hubei Province and Jianghan Plain were analyzed.The results showed that the soil pH gradually decreased from the nor
GUO He-rong , CHEN Qiong-xian , ZHENG Shao-ling , YAN Xiao-long
2007, 26(2).
Abstract:In the present study,Nutritive Soil Modifier(hereinafter abbreviated as NSM)was applied to acid soils with different character.An indoor soil incubation experiment were conducted and a greenhouse soil pot experiment with corn(Zea mays L.).The majorresults
LI Xiao-kun , LU Jian-wei , LU Jun-ming , CHEN Fang
2007, 26(2).
Abstract:A field trial was carried out in Jianghan Plain to study the effect of the combined application of N and P fertilizer on the yield and nutrients uptake of ryegrass.It was revealed that when P fertilizer was applied alone without N fertilizer,revealed the
XIONG Shuang-lian , XIONG Zhi-ting
2007, 26(2).
Abstract:利用溶液培养试验研究了镧(0.00-5.00 mg/L La)与铅(0、101、00 mg/L Pb)相互作用对雪菜幼苗生长以及铅和镧在雪菜体内累积分配的影响。结果表明,镧对雪菜幼苗根和地上部生长无明显的促进作用。当溶液中镧的浓度≥1.00 mg/L时,镧抑制了根长生长;铅抑制了雪菜根中镧的含量;当溶液中La≤1.00 mg/L时,铅还抑制了镧向地上部的累积和转移。在低水平铅污染(10 mg/L Pb)条件下,镧对雪菜铅的吸收和累积均无显著影响;但是在高水平铅(100 mg/L Pb)处理的溶液中,镧降低了雪菜根中铅的含量。100 mg/L Pb和5.00 mg/L La处理促使更多比例Pb和La向雪菜地上部转移。镧与铅在雪菜根细胞壁中存在竞争作用。
ZHAO Rui-xia , LI Bang-you , ZHOU Mu-qing , XIA Yu , LIANG Zuo-zhao , YANG Zhong-xiang , WU Xin-cheng , GONG Yan-zhang , PENG Xiu-li , LI Wen-ming , XIONG Jia-jun , YU Yong , ZHANG Shu-jun
2007, 26(2).
Abstract:以广东温氏集团培育的黄羽肉鸡种蛋胚胎为研究对象,并以与人和小鼠胚胎发育相关的胰岛素样生长因子2受体(insulin-like growth factor type II receptor,IGF2R)基因为候选基因,进行了SNP位点的筛选和多态性的检测,探讨了IGF2R的变异对胚胎死亡的影响。在IGF2R第34外显子中发现2个SNP位点,对具有AlwNⅠ酶切位点的SNP位点进行了PCR-RFLP检测,分析了胚胎IGF2R的SNP位点PCR-RFLPs基因型在死亡和正常发育胚胎中分布特征。经2χ检验,鸡基因IGF2R第34外显子AlwNⅠ-RFLP基因型在死亡和正常发育胚胎中存在显著差异,在死亡的胚胎中基因型AT的频率(0.326)显著地高于正常发育胚胎的频率(0.077)。
ZHAO Jin , SI Li-fang , XU Yong-ping , LI Wen-xian , FAN Guang-li
2007, 26(2).
Abstract:为了解外源性IFN-γ对植入前期大鼠下丘脑内IL-4表达的影响,通过阴道口肌肉注射不同剂量IFN-γ后观察植入前期大鼠下丘脑内IL-4表达的变化,并且用免疫组织化学SP法对其检测。结果表明:在下丘脑的视前大细胞核(POMA)、视前内侧核(POM)、交叉上核(SO)、视上核(SC)、室旁核内侧部(PVM)和室周核(HPV)等处均有IL-4阳性细胞的表达分布;试验1组(注射IFN-γ25 000 IU)和试验2组(注射IFN-γ75 000 IU)各个核团中阳性细胞数与对照组相比较少而且差异显著(P<0.05),2个试验组相比,在部分核团中阳性细胞数试验1组高于试验2组,差异显著(P<0.05)。结果说明下丘脑内IL-4阳性细胞随着外源性IFN-γ剂量的增加而减少,从而说明外源性IFN-γ有可能会影响下丘脑中IL-4蛋白的表达分布。
DENG Li-xin , DENG Gan-zhen , HE Cong , TIAN Chao
2007, 26(2).
Abstract:用AMI-900型兽用B超,对经过超排处理的供体羊进行实时监测,主要包括卵巢的发育,卵泡或黄体的数量、大小等,并据此判断超排效果、估测排卵数和胚胎回收数。结果表明,卵巢上卵泡发育的最佳监测时间为超排处理后的第3-4天,黄体发育监测可在手术冲胚前2-3 d进行。13例供体羊的B超监测卵泡数与回收胚胎数基本相符(142/133),说明根据监测卵泡数而预测回收胚胎数是可行的。
FU Xiu-ling , WU Hua , CHEN Hong-ying , YUAN Li , PAN Yu-shan , WU Bo-da , HU Gong-zheng
2007, 26(2).
Abstract:The antibacterial activity of various antibiotics or beta-lactams with inhibitors against non-beta-lactamase-prouding,extended-spectrum beta-lactamase(ESBLs)-producing and AmpC enzyme-producing isolates were evaluated with two-fold dilution method after p
SHI Xiu-jie , LIU Hong , GAO Long-ying , HE Jun-qiang , JIANG Yu-lin
2007, 26(2).
Abstract:Severe ulceration occurred on the head and back of cultured arctic charr(Salvelinus alpinus) in a farm of Sichuan Province in May,2004.In order to study the aetiology and prevention measures,bacteriological and molecular biology methods were used in the i
JIA Ya-dong , WANG Cheng-zhu , CHEN Chang-fu
2007, 26(2).
Abstract:Soft-shelled turtle(Pelodiscus sinensis)were immunized with crude lipopolysaccharide(LPS) extracted from Aeromonas hydrophila,Cytophaga columnaris and Edwardsiella ictaluri respectively.The immune response of crude lipopolysaccharide of Aeromonas hydrophi
JIANG Ling , CHEN Ze-xiong , XU Xiang-hua , DENG Xiu-xin
2007, 26(2).
Abstract:The effect of shoot-tip size on eradicating pathogen of Citrus Yellow Shoot by shoot-tip grafting(STG) was studied.With the binoculars and stereoscopic microscope,the meristem of shoot-tips containing growing cone and 1,2,3 or 4 leaf primordia separately
2007, 26(2).
Abstract:愈伤组织保存是柑橘种质资源保存的一条值得探索的途径,具有节省人力、物力、财力以及避免自然灾害和病虫害等优点。研究表明[1],待测的35种基因型的愈伤组织中,只有9种具有体胚发生能力。对于具有体胚发生能力的愈伤组织,它们能否长成正常的植株,这对于利用愈伤组织进行细胞融合或遗传转化等生物技术研究具有非常重要的指导意义。本试验以具有体胚发生能力的8种愈伤组织为试材,分析其体细胞胚胎发生能力与再生植株生长状态的关系,通过改变培养基配方来诱导胚性愈伤组织畸形胚状体分化,为细胞融合和遗传转化提供参考。通过鉴定再生植株的倍…
FU Chun-hua , LEI Cheng , DENG Hui , YANG Ying , LIU Shun , YU Long-jiang
2007, 26(2).
Abstract:Factors influencing adventitious bud regeneration of Glycyrrhiza hypocotyls were studied.The results indicated that KT had a prominent effect.Combined with 6-BA and IBA,adventitious bud can be directly induced from hypocotyls of Glycyrrhiza,the polarizati
2007, 26(2).
Abstract:Rhus chinensis is an important tree species with high economic value.Its hard seed-shell significantly impedes its seeds germination.In order to explore the methods to promote the seeds' germination of Rhus chinensis,researches were conducted on the effec
2007, 26(2).
Abstract:The effects of period of explants and growth regulator on the callus induction and adventitious shoot formation were investigated.The results showed that scapes within five-leaf period of Rose Supreme and seven-leaf period of Advanced Red were ideal expla
HU Hong-ju , CHEN Qi-liang , WANG You-ping , TIAN Rei , YANG Xiao-ping
2007, 26(2).
Abstract:Four sand pears,including Jinshui 2,Cuiguan,Housui,Jinshui 1,were used to investigate the changes of the contents of main sugar and acid during fruit development.The results showed that:the changes of contents of the fructose and glucose remained similar
LI Jun-li , WANG Yun-qiang , XIANG Chang-ping
2007, 26(2).
Abstract:研究了南瓜多糖体外清除自由基以及抗氧化的作用.采用水杨酸法和AP-TEMED法分别检测羟基自由基(·OH)和超氧阴离子自由基(O2-),研究南瓜多糖清除·OH和O2-的效果.用硫代巴比妥酸法测定小鼠肝丙二醛含量,用分光光度法测定小鼠红细胞溶血和肝线粒体肿胀程度,研究南瓜多糖的抗氧化效果.结果表明:南瓜多糖可以清除·OH、O2-,抑制·OH所致丙二醛的产生,减少·OH所致红细胞溶血,减轻·OH所致肝线粒体肿胀程度.试验证明南瓜多糖具有明显的抗氧化作用.
2007, 26(2).
Abstract:This paper is focused on discussion about experiencing design under economical conditions.including introduction of the main theory on experience economy and analysis of the principles and essentials,which lay a theoretical foundation for design of forest
LIU Rui , LI Cai-guo , LIU Ding-yi , PAN Si-yi
2007, 26(2).
Abstract:以大豆分离蛋白为原料提取7S球蛋白,研究了风味复合蛋白酶对大豆7S球蛋白的改性作用,并且评价了7S球蛋白改性前后的功能性质。结果表明,试验得到的7S球蛋白的持水性、乳化能力和吸油性优于大豆分离蛋白。风味复合蛋白酶酶解最佳的条件为pH值8.5,温度55℃。7S球蛋白改性后功能性质优于改性前。改性后7S球蛋白持水性有明显改善,在水解度为18.43%效果最好。吸油性、乳化性及乳化稳定性也有明显改善,在16.07%的水解度时效果最好。
HUANG Liang , HU Xian-wen , YUE Xia-li , WANG Hong , YE Fa-bing , DONG Yuan-yan
2007, 26(2).
Abstract:将甲壳素溶于NaOH/CO(NH)2水相溶剂中,研究了甲壳素水溶液的紫外光谱性质,确定甲壳素在新溶剂中的最佳紫外吸收波长为230 nm,试验了不同溶剂组分浓度、测试温度对紫外分光光度法测定甲壳素浓度的影响,发现对于不同溶剂组分浓度的甲壳素溶液,甲壳素的质量百分比浓度与紫外吸收值均有较好的线性相关性,且测定时温度在8-20℃范围内时,测定结果准确。
WU Wei-bin , HONG Tian-sheng , WANG Xi-ping , PENG Wan-xi , LI Zhen , ZHANG Wen-zhao
2007, 26(2).
Abstract:This paper reviews current knowledge concerning the use of direct and indirect ground-based measurement methods and problems for leaf area index(LAI) determination.It also compares each instrument based on indirect methods with other.Finally,the LAI measu
Tel: 027-87287364
Postcode: 430070
E-mail:hnlkxb@mail.hzau.edu.cn
Address: No.1 Shizishan Street,Hongshan District,Wuhan, Hubei,China
Jorunal of Huazhong Agricultural University ® 2024 All Rights Reserved Supported by:Beijing E-Tiller Technology Development Co., Ltd.