Abstract:A 6-phosphomannose isomerase(PMI) gene,called manA,from Escherichia coliwas cloned by PCRand used for construction of expression vectors for both prokaryotesand eukaryotes.Upon induction with IPTG a GST-PMI fusion protein was expressed in E.coliand purifi

Abstract:A highly efficient method of extracting total RNA from rapeseed was developed.Polyphenols,polysaccharides and protein,rich in rapeseed cells,could be removed effectively.Two clear bands of 28S rRNA and 18S rRNA isolated from rapeseeds could be detected by

Abstract:The fused Bt gene cry1Ab/cry1Acwas introducedinto the calli of indica TGMS lines 03B198Sand 03B199Susing Agrobacterirum-mediated method.After multiple-cycle selections withhygromycin,lots of independent transformants were obtained and at least 5 plants we

Abstract:The SLT-IIeB gene without its signal and transmembrane sequence was amplified by PCR from a Escherichia colistrain called Ee responsible for the edema disease in piglets in Hubei Province.The amplicon was cloned intothe prokaryotic expressionvector pGEX-K

Abstract:在武汉自然条件下,于2003年和2004年连续2 a观察810S、M102SI、D24、M23S和M61S 5个温敏核不育系稻蔸再生苗的花粉育性变化,结合观察期的温度变化进行育性稳定性、育性对温度的敏感时期以及育性转换临界温度的分析。结果表明,M23S和M102S的育性最稳定,810S和M102S的育性转换敏感期分别在其抽穗前的13~5 d和10~7 d,育性转换临界温度分别为24.7℃和24.0℃;ID24、M23S和M61S的育性转换敏感期分别在其抽穗前的19~10 d2、4~8 d和16~0 d,育性转换临界温度分别24.6℃、23.7℃和25.9℃。

Abstract:The relationship between population quality after heading stage and yield formation under SRI cultivation pattern was studied systematically using the high yield rice Liangyoupeijiu and Ilyou7954 as material. The results showed that SRI cultivation had no

Abstract:Different rapeseed(Brassica napus L.) varieties,such as Zhong R-888,Ning RS-1,783,Ningyou 7,Wanyouzao and Aijiazao,with different resistance to sclerotium blight(Sclerotinia sclerotiorum) were selected as materials six days after S.sclerotiorum was inocul

Abstract:The photosynthetic ecophysiological response of Jussiaea repens to two waters with different nutrient level was measured.The results showed that there were some difference between the variation of photosynthetic characteristics under different nutrient le

Abstract:In the present paper, two new species of Tetrix Latreille are described. Type specimens are kept in the Institute of Zoology, Shaanxi Normal University.

Abstract:Under the same eggs inoculation levels,and using such index as root decay,relatively gall index,relatively eggs mass index,shoot height,root length and shoot weight,the pathogenicity for 34 Meloidogyne populations from different areas in 11 provinces(muni

Abstract:Sequences of mitochondrial 16S rDNA gene were used to generate a molecular phylogeny for the Pentatomoidea based on 23 species representing 8 putative families.Phylogenetic analyses using MP(maximum parsimony),ML(maximum likelihood) and NJ(neighbor-joinin

Abstract:利用Y-型嗅觉仪测试了桔小实蝇不同生理状态下(性成熟与性未成熟)两性成虫在不同时间内对不同剂量香蕉果肉挥发物的行为反应。结果表明,香蕉果肉挥发物对性成熟与性未成熟的两性成虫均能产生显著的引诱效果,并随着剂量的增加,引诱作用增强,当测试剂量超过0.10 g时香蕉果肉即能引起两性成虫的行为反应;同时,随着剂量的增加,成虫对香蕉果肉的反应时间缩短,当测试剂量为0.1 g时,性未成熟雌虫的反应在10 min时达到显著水平,而性成熟雌虫在30 min时达到显著水平;性未成熟雄虫的反应在5 min时达到显著水平,而性成熟在40 min时达到显著水平。当测试剂量为1 g以上时,不论性成熟与否,雌、雄虫的反应均在5 min内达到显著水平。

Abstract:Pure ethanol extracts from Acorus calamus L.were tested for its repellent action and contact action against Tribolium castaneum,T.confusum,Oryzaephilus surinamensis and Cryptolestes pusillus.The result showed that A.calamus had good contact action to adul

Abstract:球孢白僵菌Beauveria bassianaVuillemin属丝孢纲、丛梗孢目、丛梗孢科、球孢白僵菌属,是一种广谱性的昆虫病原真菌,可寄生15目149科700多种昆虫和蜱螨类[1],具有致病性强、适应性强等特点,已被用于防治多种重要的农林害虫[2,3]。桔小实蝇Bactrocera dorsalis(Hendal),别名柑桔

Abstract:以红壤(可变电荷土壤)和黄褐土(恒电荷土壤)为材料,通过根垫法分组盆栽种植玉米、油菜等作物,获得2类土壤的根际土、近根际土以及非根际土样品,测定了它们的碱解氮、有效磷和有机质等含量。结果表明:在种植玉米、油菜等作物的条件下,均为根际土壤碱解氮含量最高,非根际土壤含量最小,意味着根际能活化或富集碱解氮;红壤、黄褐土非根际的有效磷含量比根际分别高28%~44%和29%~57%,表明根际有效磷存在亏缺;根际土与非根际土的有机质含量有所变化,但差异不明显。

Abstract:The background concentrations of red soil heavy metals such as Hg,Cd,As,Cr,Cu and Pb in small areas of Yingtan City,Jiangxi Province,were investigated.About 48 red soil samples and 78 paddy soil samples taken from the areas without pollution of heavy meta

Abstract:Thirty-eight oligotrophic bacteria were isolated from different environment and their 16S rDNA sequences were analyzed.Most of the isolated bacteria were closely related to genera Acinetobacter,Porphyrobacter and Brevibacterium.Among them,a strain Y11 was

Abstract:108 maleKM mice of 19~20 days were on the base of copper exhaustion for 20 days,anddivided into 3 groups with 36 per group.Feeds were supplemented with CuSO_(4) 4.5 mg/kg(counted with copper) of the first group,Cu-Lys 4.5 mg/kg(counted with copper)of the

Abstract:通过研究细胞因子GM-CSF与生长抑素pcS/2SS融合表达质粒pGM-CSF/SS诱导小鼠免疫应答的影响,探求生长抑素基因免疫新策略。将60只20日龄雄性昆明小白鼠均分为6组,分别用PBS(C0)、pGM-CSF质粒(C1)、pGM-CSF/SS质粒(T1、T2、T3)、pcS/2SS(T4)质粒口服免疫,T1~T4组的免疫剂量分别为2×106cfu/mL、2×108cfu/mL、2×1010cfu/mL、2×108cfu/mL,以上质粒均以减毒沙门氏菌为传导载体。结果显示:pGM-CSF/SS组的抗生长抑素抗体AP/AN值显著高于pcS/2SS组,阳性鼠体增重亦高于pcS/2SS组,抗体阳性率分别是71.4%和40.0%。另外,高剂量组的AP/AN值极显著高于低剂量组,显著高于中剂量组;中剂量组的AP/AN值显著高于低剂量组,高剂量组的抗体阳性率也明显高于中剂量组和低剂量组,分别为100%、71.4%和20.0%。可见,pGM-CSF/SS疫苗的免疫效果优于pcS/2SS疫苗,且pGM-CSF/SS疫苗呈剂量依赖关系。这些结果显示出细胞因子GM-CSF可以增强生长抑素DNA疫苗的免疫效果。

Abstract:Reverse transcription polymerase chain reaction(RT-PCR) and nested polymerase chain reaction(nested-PCR) were established for detectiing the infectious haematopoietic necrosis virus(IHNV).The nucleotide fragments of 786 bp and 323 bp coding part of the vi

Abstract:The pathogenic bacteria were isolated from the diseased channel catfish,Ictalurus punctatus,and identified.Healthy channel catfish were infected by injection and immersion with 19 strains of the isolated bacteria.Among the 19 strains,11 strains induced 80

Abstract:The nutritional composition in the edible part of Lamprotula fibrosa were analyzed and its nutritional quality was evaluated synthetically.The results showed that the content of crude protein in foot was 74.3%(dry basis).On the basis of dry matter of the

Abstract:Morphological factors and biomass of Schima superbaseedlings in three different habitats of environment gradients from gap's edge to gap's centre were measured and analyzed.The results showed that the difference of their morphologicalcharacter were signif

Abstract:The regeneration system of Populus euramericana San Martino was established.Leaves and petioles sprouted from the cutting shoots cultured in water were selected as explants.The results showed that on the MS medium appended with 0.5 mg/L BAand 0.1 mg/L NAA

Abstract:An extracellular polysaccharide(CEP) was extracted from submerged fermentation broth of Clitocybe gigantean.With KM mice as animal model,the method of immune organs weight of mice was used.Testing the antitumour effects of extracellular polysaccharide by

Abstract:通过对棉花秸秆干燥特性的高温综合热分析,研究了不同初含湿量的棉秆试样在不同温度条件下的定温干燥特性。结果表明:棉秆试样的干燥过程均具有预热、恒速和降速段等3段式干燥特征,且棉秆试样中所含的水分大多在预热阶段和恒速干燥阶段被脱除,干燥温度低于100℃时温度变化对棉秆试样干燥速率影响较大,而高温干燥阶段温度变化的影响明显减弱。

Abstract:Phytochelatins(PCs) can play important roles in the detoxification of toxic heavy metals and in the regulation of intracellular concentrations of essential metals in eukaryotes.At present,the biosynthetic regulation of PCs and its mechanism of detoxificat