Abstract:根据丝氨酸/苏氨酸蛋白激酶类催化结构域Ⅰ和Ⅸ的氨基酸保守序列设计简并引物,PCR扩增‘紫花芒’杧果嫩绿色叶片基因组DNA。在NCBI中用BlastX比对发现,有6个阳性克隆是抗病基因同源序列,并命名为PK1~PK6,GenBank注册序列号为AY693369~AY693371和AY776277~AY776279。在PK3、PK4和PK6中发现了丝氨酸/苏氨酸蛋白激酶的9个催化结构域Ⅰ~Ⅸ,而其它3个克隆具有部分结构域。氨基酸序列同源性分析还发现,6个克隆编码的氨基酸序列都与受体样蛋白激酶有较高的氨基酸序列同源性。系统进化树分析还表明它们都是可能的抗病基因同源序列。总之,6个克隆不仅是抗病基因同源序列而且是可能的受体样蛋白激酶基因同源序列。

Abstract:从毛白杨(Populus tomentosa)木质化茎中提取总RNA,利用RT-PCR技术进行cDNA扩增,获得1条1 621 bp的片段。测序结果表明:该片段编码536个氨基酸组成的多肽,序列中包含所有已知4-CL蛋白的2个特征序列boxⅠ(SSGTTGLPKGV)和boxⅡ(GEICIRG)。将cDNA克隆到原核表达载体pET-32a( )中,并转化大肠杆菌(Escherichia coli)感受态细胞BL21(DE3)。IPTG诱导后,经SDS-PAGE和Western-blot分析表明,4-CL基因在大肠杆菌BL21中获得表达,所表达融合蛋白的分子量约为70 ku。酶活性分析表明:该重组蛋白对底物PA、FA、CA均表现出催化活性,其偏爱性顺序为PA>FA>CA,对SA则没有活性。

Abstract:Reagents such as glycerol,DMSO,formamide are usually used to improve the efficiency of PCR for the template with high G C PCR.Much of the work is focused on one or two reagents ' effect on PCR,rather than the combination of more reagents or the influence

Abstract:人白细胞介素4(hIL4)是一种重要的免疫活性调节分子,与一些自身免疫性疾病有重要关系。将hIL4基因融合到衣藻叶绿体rbcL 5-’UTR和3’-UTR之间,组成5’UTR-hIL4-3’UTR片段,将其插入到来源于衣藻叶绿体基因组5.7 kb同源片段中,构建成pXhIL4同源重组质粒。利用电脉冲法将该质粒与含有aadA基因的壮观霉素抗性质粒p228共同转化衣藻叶绿体,从含100μg/mL的壮观霉素抗性平皿上筛选到阳性藻落,并通过PCR方法检测到hIL4基因,其共转化频率高达90%。经过western blotting印迹转移分析,证实了人白细胞介素4基因在衣藻叶绿体中获得成功表达,为进一步研究在衣藻叶绿体中高效表达外源蛋白奠定了基础。

Abstract:流行性乙型脑炎(Japanese encephalitis virus,JEV)是一种中枢系统人畜共患急性传染病,由蚊为媒介传播,以高热和狂暴或沉郁等神经症状为特征[1],猪患乙型脑炎的主要症状表现为怀孕母猪流产,产死胎或弱仔,公猪则患睾丸炎,仔猪呈神经症状,使养猪业遭受巨大损失。1988年我国学者方

Abstract:Ethylene release rate was determined in flourishing phase after treating Napus SW 0742 oil rapeseed with different concentrations of Huashaling.The changes of ethylene release rate and its relation with male sterility were studied.The result showed that t

Abstract:Two-years' experiments in double-cropping rice field were conducted.Results showed that the ditch no-tillage and straw returning,compared with the conventional tillage,made rice grow faster and the tillering stage end earlier.But the filling and productiv

Abstract:根据病原菌致病性、生物学特性及形态学研究结果,鉴定湿地松猝倒病的病原菌为丝核菌属的立枯丝核菌(Rhizoctonia solaniKühn.)。该菌的生长温度为10~35℃,以25~30℃最适宜;病菌生长的pH值范围是4~11,以pH 7~8最适宜;以蔗糖或麦芽糖为碳源,硝酸钾或硝酸钠为氮源的培养基,有利于菌丝生长;光照对菌丝生长有利。

Abstract:稻瘟病(Magnaporthe grisea)历来是湖北省各稻区危害较严重的病害之一,已造成很大的经济损失。为了解稻瘟病致病性分化,提高湖北省抗病育种的效率,合理布局抗病品种并延长其寿命,笔者连续几年用中国鉴别品种对从全省各稻区采集来的标本上分离的稻瘟病菌单孢进行了鉴定。1材料与

Abstract:The host selection of Liriomyza sativae Blanchard to different cucumber varieties(Cucumis sativus L.) was tested in the laboratory and field using choice and no-choice tests.It was found that the selection difference was mainly presented in the average nu

Abstract:通过田间试验,对稻-鸭-鱼共栖生态系统中水稻纹枯病的发生规律进行了研究并建立了相应的数学模型。结果表明:稻-鸭-鱼共栖生态系统中纹枯病发病高峰期的病株率为16.07%,比对照的病株率76.37%低60.30%,控制效果达79%。对照区、稻-鱼区和稻-鸭-鱼区三者的感染系数分别为σ=212.106 1、σ=23.945 8和σ=8.352 0。

Abstract:Result of gamma irradiation on eggs and nymphs of citrus rust mite(Phyllocoptruta oleivora Ashmead) showed that 100 Gy caused direct death of eggs and the alive eggs with hatching failure.To protonymphs,100 Gy was enough to cause a full mortality while to

Abstract:The portion clays' particle group size distribution fractal dimension in South China using digital micro-analysis was measured.A new method to calculate the soil's clay partial group size distribution fractal dimension was proposed.Moreover,the relationsh

Abstract:Parameters of rice N-nutrition and ricegrowing status were obtained with exchangeable resin and digital camera.Results indicated that the available-N from resin sack correlated with that from soil and crops growth information collected from digital camera

Abstract:从屠宰场收集黄牛卵巢,取皮质深层卵母细胞进行体外成熟、体外受精和早期胚胎体外培养,分析影响其效果的因素。结果表明,在成熟培养液中添加促卵泡素(FSH)(10 IU/mL)、人绒毛膜促性腺激素(HCG)(20 IU/mL)和17β-雌二醇(17β-E2)(1μg/mL)对卵母细胞受精后早期胚胎发育能力有极显著的促进作用。等量牛卵泡液(BFF)与初生犊牛血清(NCS)效果差异不显著,以添加15?F为最宜。颗粒细胞与输卵管上皮细胞均能显著提高卵母细胞体外成熟受精后早期胚胎的发育率,颗粒细胞 输卵管上皮细胞对克服胚胎阻滞现象效果最显著。

Abstract:A total of 65 crossbred piglets(Duroc Large White Landrace) weighing 22.5 kg were allotted,based on weight and sex,to five dietary treatments with 13 replicate pens each and 1 pig per pen as a replicate.The five dietary treatments were diet 1 based on cor

Abstract:选取体重约25 kg的长×大二元杂交猪60头,采用单因子完全随机设计,分为5组,3个重复,在饲粮中添加不同比例的苜蓿草粉。在正试期开始后的第1、20和40天称试验猪的体重,分析其饲喂含苜蓿草粉饲粮后的主要生产性能。在正试期开始后的第20天采取猪的血样,分析其饲喂苜蓿后血液中主要生化指标。结果表明:(1)添加5%,10%苜蓿草粉有利于提高生长猪的增重及饲料转化率,尤其10%组的日增重、日采食量较对照组有极显著改善,饲料转化效率较对照组明显提高。而15%、20%的苜蓿草粉组的上述指标比对照组降低。(2)添加苜蓿草粉后,饲粮干物质、粗蛋白质、NDF的消化率提高。(3)添加苜蓿草粉后,GH、Ins和IGF-1的含量显著提高,但试验组中的T3、T4含量显著降低;随着苜蓿草粉添加水平的提高,血液中尿素氮、甘油三酯、血糖和胆固醇含量递减,其中试验II组胆固醇含量和对照组相比差异显著,试验III、IV组与对照组相比差异极显著。而总蛋白的含量随草粉添加量上升有所提高,尽管各组间无显著性差异。上述结果表明:在生长猪饲粮中添加10%紫花苜蓿草粉后对生长猪生产性能、营养消化率及血液生化指标有较显著的改善。结合生产实际,建议在生长猪饲粮中添加10%紫花苜蓿草粉。

Abstract:To study the efficiency of sperm-mediated gene transfer to produce transgenic porcine,the hCD59 gene were mixed with liposome,and two methods were used to produce transgenic porcine.Firstly,the gene/ liposome complex were directly injected into male swine

Abstract:复口吸虫在我国东北、华东、华中、华南等地均有分布,能引起淡水鱼类复口吸虫病的广泛流行,其中尤以华中一些省份为甚[1-2]。研究发现引起华中地区复口吸虫病流行的主要是倪氏复口吸虫(Dip-lostomum niedashui)和湖北复口吸虫(D.hupe-hensis)[1]。为了对尾蚴和后囊蚴的形态分类

Abstract:采用单因子梯度设计方法，将维生素E设6个添加水平，分别为Ⅰ0mg／kg、Ⅱ50mg／kg、Ⅲ125mg／kg、Ⅳ200mg／kg、Ⅴ275mg／kg、Ⅵ350mg／kg，试验期共175d，以雌鳝性腺系数、产卵力、孵化率以及各组织中SOD活性、MDA含量等指标作为判据，研究了VE对雌鳝繁殖性能的影响。结果表明，随饲料中VE含量的增加，Ⅰ、Ⅱ、Ⅲ、Ⅳ组雌鳝性腺系数、产卵力和孵化率显著提高，Ⅴ、Ⅵ组则提高不显著，卵中VE的含量反映了饲料中VE的含量。雌鳝卵巢中SOD活性，Ⅳ、Ⅴ和Ⅵ组比Ⅰ、Ⅱ和Ⅲ组显著降低；相应地，MDA含量Ⅳ、Ⅴ和Ⅵ组显著低于Ⅰ、Ⅱ和Ⅲ组。Ⅰ、Ⅱ和Ⅲ组雌鳝卵巢中SOD活性较高，但因饲料中抗氧化性的VE缺乏或不足，引发卵子脂质过氧化，MDA含量显著升高，导致卵质低下，从而影响了卵子孵化。以上结果表明，雌鳝饲料中添加维生素E，能有效地改善雌鳝的繁殖性能，其最适添加量为200mg／kg。

Abstract:The effect of different plantlet,basic medium,dark culture,hormone combination and densities on the formation and differentiation of callus ofEucalyptus smithiiL was studied.It was found that callus form well in the dark using true leaf and bud as plantle

Abstract:根据丝氨酸／苏氨酸蛋白激酶类催化结构域Ⅰ和Ⅸ的氨基酸保守序列设计简并引物，PCR扩增‘紫花芒’杧果嫩绿色叶片基因组DNA。在NCBI中用Blast X比对发现，有6个阳性克隆是抗病基因同源序列，并命名为PK1～PK6，GenBank注册序列号为AY693369-AY693371和AY776277-AY776279。在PK3、PK4和PK6中发现了丝氨酸／苏氨酸蛋白激酶的9个催化结构域Ⅰ～Ⅸ，而其它3个克隆具有部分结构域。氨基酸序列同源性分析还发现，6个克隆编码的氨基酸序列都与受体样蛋白激酶有较高的氨基酸序列同源性。系统进化树分析还表明它们都是可能的抗病基因同源序列。总之，6个克隆不仅是抗病基因同源序列而且是可能的受体样蛋白激酶基因同源序列。

Abstract:Two-year-old Osmanthus fragrans Lour.was potted to study the physiological responses to different water stress.The results were as followings:The tissue water content,free water content and the ratios of the free water content to bound water content decre

Abstract:Sagittaria potamogetifolia Merr.is a vulnerable species of aquatic plant with limited distribution in the Southern China.Clonal diversity and population differentiation of four populations(sampled from Hunan,Jiangxi and Fujian)were analyzed with allozyme.

Abstract:通过MTT细胞增殖抑制试验,测定肿瘤细胞膜唾液酸(SA)含量、线粒体跨膜电位(ΔΨm)、细胞培养液中NO和NOS含量等的变化,研究菹草类胡萝卜素(carotenoids extracts fromPotamogeton crispusL.,CEPC)对人宫颈癌Hela细胞株增殖的影响及其作用机制。结果表明:CEPC对Hela细胞增殖有明显的抑制作用;能显著降低Hela细胞表面的SA含量,诱导Hela细胞ΔΨm下降,导致iNOS活性增强,NO含量显著升高。CEPC对Hela细胞的生长抑制作用可能是通过降低肿瘤细胞膜SA含量,使线粒体跨膜电位下降i、NOS的活性增强、NO的浓度升高而引起肿瘤细胞凋亡。

Abstract:The volatiles in cured fish were extracted through the solid phase micro-extraction fiber and identified by GC-MS.Results indicated that pickling of cured fish could accelerate the transformation of volatiles and their precursors into the corresponding fl

Abstract:A data object access model combining the object-oriented programming technique with the access process of traditional relational database management system(RDBMS) was put forward.The results indicated that the model can simplify the access process of RDBM

Abstract:The diversity of genetics and plasmids,functions of plasmids,Nod factors,nod genes and exopolysaccharide in Mesorhizobium huakuii were reviewed.Mesorhizobium huakuii could only form nodules in Astragalus sinicus L.,and was incorporated in Mesorhizobium in