Yang Xiuping Liu Huanzhang Liu Bingyin
2002, 21(2).
Abstract:In the present paper, specimens of Zacco platypus (Temminck et Schlegel) collected from 13 areas were researched by the methed of the frame and morphomentric and principle component analysis was conducted. The result showed that main morphological differe
Cai Mingli Lu Kaiyang Chen Peng Zhao Heming Huazhong Agricultural University , Wuhan , China Hubei Seed Management Station , Wuhan , China
2002, 21(2).
Abstract:In the experiment, late indica hybrid rice Jinyou928 was studied by means of quadratic orthogonal regressive rotation combination designed with four factors including planting density, nitrogen fertilizer, phosphorous fertilizer and potassium fertilizer,
2002, 21(2).
Abstract:棉花病虫害是制约棉花高产稳产和提高棉花品质的主要因素,减轻病虫对棉花的危害,节省防治投资、提高防治效果,尤其是进一步保护环境、减少农药对环境的污染,保持棉田生态平衡,争取获得经济效益、社会效益和生态效益同步提高是棉花科技工作者的重要任务。为了实现这一目标,必须建立
Fang Yusheng Zhao Jun Zhuo Kan
2002, 21(2).
Abstract:Bursaphelenchus dongguanensis sp. n. is described and illustrated. Both sexes have a long, slender body (a=64-134) and a short tail (c=48-102). The female vulva lacks a flap and is more posterior than with other species (V=86-92). The conoid tail of both
Hong Lifang Fu Libo Zhao Zongsheng Guo YurongZhang Jun Chen Peng Liu Wuding
2002, 21(2).
Abstract:The relationship between K accumulation of roots and endogenous and exogenous auxin was conducted by pot culture. Under the condition of no applied K, exogenous auxin helped accumulation of root-K at different growing periods, while endogenous auxin stabi
Miao Lihong Ma Xiangdong Zhou Junchu
2002, 21(2).
Abstract:Sinorhizobium fredii HN01 is a cultivar non-specific nodulation strain on soybean. The symbiotic plasmid pSymHNOlb of HN01 was deleted about 40 kb when it mobilized into WWG18SR which is another cultivar non-specific strain of S. fredii. The pot plant tes
Zhao Junlong Chen Huanchun Lii Jianqiang Zhou Fuchun
2002, 21(2).
Abstract:On the basis of single PCR of PPV and PRV, multiple PCR-based diagnosis method was studied. The results showed that two specific fragments of 445 bp for PPV and 217 bp for PRV could be amplified in a single PCR. This method could differentiate PRV infecti
Gu Changqin Cheng Guofu Hu Xueying Xie Changqing Song Nianhua
2002, 21(2).
Abstract:8周龄健康肉鸡60只随机分成2组,试验组(45只)经关节腔注射鸡源致病性金黄色葡萄球菌2.5×109cfu,复制鸡葡萄球菌性关节炎的病理模型,对照组(15只)经关节腔注射PBS。对所建立的病理模型进行临床症状观察、外周血内细菌、白细胞及其分类计数以及抗体水平的动态研究。结果显示:试验组接种后均发展为典型的关节炎症状。整个试验期内试验组的死亡率为51.11%,对照组表现正常。接种后1-5 d外周血内金黄色葡萄球菌迅速升高。试验组鸡接种后血液中白细胞(WBC)总数升高。试验组和对照组的WBC总数的差异水平在第1、5、7、21、28 d极显著(P<0.01)。异嗜性细胞和淋巴细胞总数在接种后1周内明显升高,与对照组相比差异水平极显著(P<0.01);单核细胞在第3 d上升至11.67%,显著高于对照组。淋巴细胞的转化率在接种后第3、5、7 d的转化率极显著的高于对照组(P<0.01),第7 d达到峰值70.30%。试验组的抗体水平在整个试验期内呈缓慢增长趋势。
Hu Gongzheng Xu Lanju Yuan Li Li Min Yuan Baoqing Deng Qiang
2002, 21(2).
Abstract:测定了左旋氧氟沙星对家禽致病菌的抗菌活性和抗菌后效应(PAE),观察了其对人工感染鸡葡萄球菌病的治疗效果。结果表明:左旋氧氟沙星有广谱抗菌作用,对家禽常见致病菌(革兰氏阳性,阴性菌)均有高度的抗菌活性。对鸡大肠杆菌O74等标准菌株的MIC介于0.05-0.20μg/ml,而对临床分离菌株的MIC介于0.20-0.80μg/ml之间。左旋氧氟沙星等氟喹诺酮类抗菌药物对革兰氏阳性,阴性菌均产生明显的PAE,并呈浓度依赖性。左旋氧氟沙星对人工感染鸡葡萄球菌病有良好的治疗效果,其分别按每升水25mg,50mg,100mg及氧氟沙星按每升水50mg混饮4d,治愈率分别为81.3%,90,6%,68.8%,感染对照组的死亡率为59.4%。
Wang Daju Zhou Shiqi Kong Ke Fan Shengxian Cao Jiyue Yang Hui
2002, 21(2).
Abstract:The wistar rats were used to study the sub-chronic toxicity of sarafloxacin hydrochloride. The drug was given to 4 groups of animal at levels of 218. 8 mg/kg, 109. 4 mg/kg , 21. 8 mg/kg (equal to 200 mg/kg, 100 mg/kg , 20 mg/kg of sarafloxacin) and 0 by o
Liu Guoyong Mao Zhijuan Chen Chaoran Chen Changfu
2002, 21(2).
Abstract:Formalin and phenol and heat which killed cells of Vibrio harveyi were prepared to show immunogenicity to silver crucian carp,Carassius auratus gibelio by examination of agglutinating antibod-y titres of serum as well as phagocytic activity of blood leuco
2002, 21(2).
Abstract:二氧化氯(ClO2)因为具有良好的消毒效果而被广泛用于生活用水和工业循环冷却水处理、医疗卫生消毒、环境保护以及石油、造纸、食品等许多领域,也已被证明是一种良好的水产养殖用消毒剂。然而,由于ClO2制备方法和剂型较多,如何评价ClO2的消毒效果,不同的研究者所采用的方法各异。笔者通过测定供试鱼鳃片上附着菌数量,比较了不同浓度的ClO2溶液浸泡消毒对附着菌的清除作用,旨在将ClO2科学地应用于水产养殖业中提供依据。
Ai Pengfei Luo Zhengrong {Huazhong Agricultural University , Wuhan , China)
2002, 21(2).
Abstract:The factors influencing rooting of nonastringent persimmon(Diospyros kaki L. f. ) shoots in vitro were studied by orthogonal design. The result showed that the shoots subcultured constantly on the MS(1/2N) medium supplemented with CPPU 0. 2 mg/L were dipp
2002, 21(2).
Abstract:黄姜(Dioscorea zingiberensis C H.Wright)是薯蓣科薯蓣属植物,因其含有较高的薯蓣皂甙元,而成为我国生产薯蓣皂素的主要原料。近年来随着野生资源的逐渐枯竭,不少地区已开始人工栽培。以前对黄姜的研究,侧重于工业利用、组织培养和人
Zhao Siming Xiong Shanbai Zhang Shenghua
2002, 21(2).
Abstract:The rheological properties and texture of rice starch paste during retrogradation were studied by using a rotary rheometer and discrepant microscope. Results indicated that rice starch paste displayed the character of pseudoplastic fluid, and showed shear
Ni Xuewen Wu Moueheng Ding Keyong
2002, 21(2).
Abstract:对RP-HPLC分离银杏酚酸的各影响因素进行研究,分析了流动相组成、pH值和温度对银杏酚酸各组分色谱保留行为的影响,得到优化分析条件为色谱柱:SUPELCOSILTMLC-18,5 cm×4.6 mm,5 μm;流动相:90%甲醇,乙酸pH值为3.15;紫外检测波长λ=310 nm;流速0.7 ml/min;AUFS=0.02;柱温30℃。在确定的分离条件下,银杏酚酸各组分能很好的分离。
Su Qingyun Yan Mei Guo Zuming Zhao Chengyi
2002, 21(2).
Abstract:Crude corn oil was extracted from corn grown in Enshi ,a district rich in selenium of China. The content of selenium in the crude oil was 22 times higher than an usual corn oil. Among the products obtained from the oil extraction and saponification separa
Li You Zhao Yong Dong Zhongqiang {Henan Agricultural University , Zhengzhou , China)
2002, 21(2).
Abstract:According to the fact that the sunlight absorbent-side of the sunlight greenhouse inclining to the sun caused the amount of sunlight increasing, the concept of raising-temperature coefficient and the mathematical model was raised and the rule of raising-t
2002, 21(2).
Abstract:光合作用是作物高产的生理基础。关于小麦旗叶光合作用对籽粒发育和产量形成的作用,前人已有较多报道。但以往的研究试验的品种有限,测定的旗叶生理指标较少,更没有系统分析旗叶光合生理指标与光合产物——叶片含糖量之间的关系。以湖北省大面积种植的小麦品种和育成的新品系为材料,研究不同粒型品种旗叶光合生理指标的差异及其与叶片含糖量的关系,可丰富小麦高产育种和栽培理论。
2002, 21(2).
Abstract:红豆杉细胞培养生产紫杉醇,是一种潜在的大规模生产紫杉醇的重要途径,然而和其它植物细胞培养一样,红豆杉细胞的紫杉醇含量较低等问题,限制着该技术的产业化进程。寻找提高和稳定紫杉醇产量的方法,是目前需要解决的主要问题。筛选合适的生物或非生物诱导子(如真菌培养物、茉莉酸甲酯、水杨酸、Cu2+、Ce4+、Ag+),提高细胞的紫杉醇产量,成为国内外研究的热点。
2002, 21(2).
Abstract:磷脂酶A2(Phospholipase A2,PLA2),即磷脂-2-酰基水解酶(EC 3.1.1.4),广泛存在于生物体内,是磷脂分解代谢中起重要作用的酶。它催化甘油磷脂C-2位脂肪酸的水解反应,生成溶血磷脂和脂肪酸。PLA2具有重要的生理意义,在代谢过程中能够消化磷脂分子,而且在激素的信号传递以及发炎过程等方面都起重要作用。PLA2也具有广泛的应用价值,可以用于生物膜构造和机能的研究、溶血磷脂的制造、油脂精炼、疾病治疗、饲料添加剂制造等方面。
Li Xiaoding Wu Moucheng Zeng Xiaobo Rong Jianhua Wang Zhongmin
2002, 21(2).
Abstract:灰树花子实体用热水提取,乙醇沉淀,透析冻干得灰树花多糖粗品,再经Sevag法脱蛋白,DEAE-Sephadex A-25柱层析纯化得到4种多糖分别为PGF-1,PGF-2,PGF-3和PGF-4。4种多糖经纸层析,Sephadex G-200柱层析及聚丙烯酰胺凝胶电泳分析。结果表明都为单一均匀组分:PGF-1经纸层及气相色谱分析证实它是一种葡萄糖;凝胶渗透色谱测定其分子量为11万。红外光谱揭示PGF-1含β-型糖苷键。
Chen Chunli Guo Wenwu Deng Xiuxin {Huazhong Agricultural University , Wuhan , China)
2002, 21(2).
Abstract:The technique of chromosome in situ hybridization (CISH) has been extensively applied to genetics and breeding of higher plants. In genetic identification of somatic hybrids of higher plants, CISH has been used to detect the origin and ploidy variation, c
Li Qing Liu Hanlan Chen Hao {Huazhong Agricultural University , Wuhan , China)
2002, 21(2).
Abstract:Analysis of pesticide residues in Chinese herbal medicine and its recent development are reviewed. Solid-phase microextraction, capillary electrophoresis and supercritical fluid extraction are widely applied in pre-treatment of sample. Coupled technology
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