Li Maoteng ) Cai Detian ) Huang Limin ) Ma Pingfu )
2001, 20(3).
Abstract:Studies were been made on the biological characteristics and the variation of chromosome numbers of root tip in BC 2F 2 offsprings between wheat and wheat grass. The hybrid's biological characters are between wheat and wheat grass, but are more closel
Cheng Zhongping ) , ) Chen Zhiwei ) Hu Chungen ) Deng Xiuxin ) \ Luo Zhengrong )
2001, 20(3).
Abstract:采用RAPD技术,用筛选的22个随机引物对桃属主要种类及其类型进行DNA扩增分析,结果表明:不同引物扩增出的DNA片段范围在3-13条,分子量范围在300bp-3dkb之间,依据特征谱带,可建立种间及其类型识别的分子检索表,利用22个引物扩出的180个位点上的BAPD带,进行聚类分析,结合前人研究出的结果,揭示桃种间亲缘演化顺序为:内蒙古长柄扁桃→光核桃→山桃→甘肃桃→白花山桃、红花山桃、帚形山桃→桃巴旦、陕西桃巴旦→新疆桃、毛桃。
2001, 20(3).
Abstract:采用RAPD技术,用筛选的22个随机引物对桃属主要种类及其类型进行DNA扩增分析。结果表明不同引物扩增出的DNA片段范围在3~13条,分子量范围在300bp~3kb之间,依据特征谱带,可建立种间及其类型识别的分子检索表。利用22个引物扩出的180个位点上的RAPD带,进行聚类分析,结合前人研究出的结果,揭示桃种间亲缘演化顺序为内蒙古长柄扁桃→光核桃→山桃→甘肃桃→白花山桃、红花山桃、帚形山桃→桃巴旦、陕西桃巴旦→新疆桃、毛桃。
2001, 20(3).
Abstract:对小麦(Triticum aestivum)和无融合生殖小麦草(Elymus rectisetus)的杂种原形代(BC2E2)生物学特性和根尖染色体数目变化情况进行了研究,结果表明,BC2F2的生物学特性处于小麦和小麦草之间,但更趋向于小麦,这晨由于杂交后代经2次回交所致,根尖染色体检查发现,BC2F2的体细胞染色体数目存在1个很大的变异范围,从29条到69条不等,但大部分细胞内的染色体数目是42条,杂种后代的体细胞染色体数目的变化一方面与远缘杂交有关,另一方面可能与无融合生殖具有一定的联系。
2001, 20(3).
Abstract:The parental male sterile line 1141A of the pol CMS high quality hybrid "Huaza No.4" of Brassica napus L. was genetically improved by utilizing "the plant nuclear and cytoplasmic male sterility(RGCMS) theory" proposed by the authors. The newly bred R
Zhang Jiaohai Bie Shu Tang Shifang Wang Shaohua He Xingjiao\ Wang Xiaogang
2001, 20(3).
Abstract:Studies were carried out on the soil physical characters, roots vigor, flower bud differentiation, accumulation and contribution of dry matter, changes of leaf area and lint yield of transplanted cotton covered with plastic film (T
Cheng Guoying Tu Lichao Yang Guogui Zhou Huazhong
2001, 20(3).
Abstract:Comparing of the phage isolation methods of the maceration extracts of the diseased leaves treated with bacterial filters, chloroform and the original maceration extracts without any treatment respectively, the result indicated that the treatment with a b
2001, 20(3).
Abstract:卢斯垫刃线虫新种 (Tylenchuslucin .sp .)和广东垫刃线虫新种 (Tylenchusguangdongensisn .sp .)分别采集于广东省深圳光明农场的玉米 (Zeamays)和宝安马田菜场的葱 (Alliumfistulosum)根际土壤。前者以角质层无明显环纹、有明显的后阴子宫囊等特征明显区别于相似种小垫刃线虫 (TylenchusminorXie&Feng ,1997)和拟小垫刃线虫 (TylenchusparaminorXie&Feng ,1997) ;以口针长 8.3~ 10 .4μm、后阴子宫囊明显和尾短于或等于肛阴距等特征明显区别于相似种微针垫刃线虫 (TylenchusstylolusXie&Feng ,1995 )。后者以头部环纹明显、后阴子宫囊显著等特征区别于相似种小垫刃线虫 (T .minor)和拟小垫刃线虫 (T .paraminor) ;以尾长于肛阴距 ,交合刺较长等特征区别于相似种卢斯垫刃线虫新种 (T .lucin .sp .)和亚戴维恩垫刃线虫 (T .subdavaineiMukhina,1981)。
2001, 20(3).
Abstract:针对月季栽培过程中出现的最严重的根结线虫开门见山防治问题,开展了月季对根结线虫抗性遗传研究,研究结果如下:(1)对常用的4种月季砧木R.multiflora K1,K2,CE63及R.indica对左面种常见根结线虫即南方根结线虫(Meloidogyne incognita)、瓜哇根结线虫(M.javanica)、花生根结线虫(M.arenaria)及北方根结线虫(M.hapla)的抗性进行了测定,得出只有北方根结线虫能侵染月季,其余3种不能侵染。(2)根结指数与对数转化后的每克根中雌虫数、雄虫数、3-4龄幼虫数之和、2龄幼虫数、卵数、成虫有为3-4继幼虫数之总和、2龄幼虫与卵数之总和均呈线性相关关系,因此根结指数是进行抗性评价的可靠、简单及实用的指标,抗性划分标准如下:根结指数0.0-1.0,抗病;1.0-2.0,中等抗病;2.0-3.0,感病;3.0-4.0,比较感病;4.0-5.0,高度感病。(3)以根结指数为指标,通过对杂交所得74个子代的抗性评价试验表明;在2抗性亲本所得12个子代中,各子代抗性水平存在抗性分化,说明抗病基因不晨由单一显性基因控制;在所有的62个抗性亲本与感病亲本的杂交子代中,59个子代均比抗病亲本感病,表明抗病基因由隐性基因控制,因此,提出了月季对根结线虫的抗性遗传可能由2个基因决定。
Wen Yanhua Feng Zhixin Xu Hanhong Chen Li
2001, 20(3).
Abstract:Extracts of 14 Chinese plants (belong to 10 families in Hubei,Shanxi and Guangdong Provinces, China) were assessed for nematicidal activity against Bursaphelenchus xylophilus using a fungal feeding method in vitro. Some of 14 plant extracts were also
2001, 20(3).
Abstract:茯苓喙扁蝽Mezira(zemira)poriaicolaLiu ,又称茯苓虱 ,属于半翅目 (Hemiptera) ,扁蝽科 (Araidae) ,是近年来在茯苓产区发现的一种为害十分严重的害虫。茯苓 (Poriacocos)是一种重要中药材 ,主要出产在我国的一些山区。茯苓除满足我国人民需要外 ,还出口东南亚等国。因此 ,发展茯苓生产对保证人民身体健康 ,增加山区农民收入和出口创汇具有重要意义。但茯苓喙扁蝽的严重为害成为发展茯苓生产的极大障碍。国内外对这种害虫很少 ,研究仅见关于成虫形态的描述[1~3] ,目前对这种害虫的发…
Duan Haiyan Wang Yunhua Xu Fangsen Liu Hui
2001, 20(3).
Abstract:Genotypic differences of phosphorus(P) efficiency among 194 Brassica napus L. cultivars were investigated under pot culture in acid soil with a method of two step screening typical cultivars. P efficiency coefficient(the ratio of dry weights or seed
2001, 20(3).
Abstract:丛枝菌根真菌能与多数旱地植物共生 ,形成菌根。菌根的形成能促进植物对营养元素 ,特别是磷素的吸收 ,并增强植物的多种抗逆性 ,从而达到促进植物生长 ,提高产量的目的。水稻是我国的主要粮食作物 ,需要在淹水条件下生长 ,而菌根真菌是好氧性微生物 ,在淹水条件下不能形成菌根 ,因而至今还未见到将菌根真菌应用于水稻栽培的报导。但是在旱育条件下 ,能否通过接种菌根真菌 ,帮助秧苗形成菌根 ,培育出健壮的秧苗 ,从而提高水稻的产量 ?这是本试验的目的。在测试菌根真菌效果的同时 ,为了探讨菌根真菌与细菌肥料的协同关系 ,在试验中加入了细菌…
2001, 20(3).
Abstract:The serological relationship between 8 strains of Bradyrhizobium japonicum isolated from the north east area of China and 15 standard serotype strains were compared with the cross agglutination reaction method. It was found that strains 93H10F5 and
Liu Jianzhong ) Xiong Yuanzhu ) Li Ning )
2001, 20(3).
Abstract:In this article,the following two questions was answered: (1) Could transgene(s) be transmitted to the offspring of the transgenic animals? And (2) if could, how to inherit? Two female transgenic mice were mated with nontransgenic male mice. After full t
Yi Jianming ) Li Shucong ) Ji Zhaojun ) Xia Xuanyan ) \ Yan Bangfu ) Mao Zhaoxun ) Wu Shiqin )
2001, 20(3).
Abstract:The milk yield records of Holstein cows on the Bayi and Wuchang dairy farms from 1995 to 1999 in Wuhan were collected and adjusted to standard lactation length of 305 days. One year was divided into three seasons considering the special climatic character
Gao Tengyun ) Wang Yanling ) Han Zhengkang ) Yang Xuefeng ) Wang Laoqi )
2001, 20(3).
Abstract:and 16 beef cattle were assigned to experimental group and control group to carry out a feeding trial by adding 20 mg/kgBW cysteamine in the ration of the experimental group only. The results showed that cysteamine could significantly increase the weight
2001, 20(3).
Abstract:研究发现由于雏鸡消化道内无 β 葡聚糖酶 ,在采食大麦日粮后 ,其中所含的 β 葡聚糖使食糜的粘性增加 ,阻碍了内源性消化酶与营养物质的相互作用 ,导致消化率下降 ,影响机体正常生长 ,同时增加粪便的排泄物量 ,加重了对环境的污染[1 ] 。在对生长鹅的研究中发现 ,大麦日粮添加含有 β 葡聚糖的粗酶制剂 ,能解除大麦中 β 葡聚糖等可溶性非淀粉多糖的抗营养作用 ,改善消化 ,促进吸收 ,从而提高机体对大麦的利用 ,加速生长[2 ,3] 。目前生产中主要是采用添加外源性酶制剂来解除 β 葡聚糖的抗营养效应 ,以提高大麦的生物利用价值和机体的生产…
Chen Mingyong ) Chen Dewei ) Zeng Qunhui )
2001, 20(3).
Abstract:A enterotoxigenic Escherichia coli was isolated from died yak with diarrhea in Tibet area. The strain had almost the same morphological, cultural and biochemical characteristics as Escherichia coli . The serotype of the strain was O148. The isolate
2001, 20(3).
Abstract:沙拉沙星 (Sarafloxacin)是新型氟喹诺酮类抗菌药物 ,1 995年首次在美国上市 ,是FDA批准可用于食品动物的专用品种。其抗菌谱广、对革兰氏阳性菌、阴性菌均有高度的抗菌活性 ,在组织中的残留期短 ,是防治家禽大肠杆菌病、沙门氏菌病、霉形体病及霉形体与细菌混合感染的有效药物。为保证国产沙拉沙星的安全应用 ,我们按农业部有关规定对其进行了毒理学评价 ,为临床安全用药提供科学依据。1 材料与方法1 .1 药品与试剂沙拉沙星原粉 :含量 98.5 % ,批号 970 31 5 ;沙拉沙星口服液 ,浓度 1 % ,批号 970 31 5 ,均由洛阳惠中兽药…
2001, 20(3).
Abstract:采用神尔马林灭活的嗜水气单胞菌(Aeromondas hydrophila)菌苗免疫接种异育银鲫,通过测定受免鱼血清中凝集抗体效价,溶菌酶活性,血液中吞噬细胞的吞噬活性,杀菌活性并通过鱼体攻毒试验,探讨了异育银鲫对福尔马林灭活的嗜水气单胞菌菌苗的免疫应答状况,结果表明,接种28d后,受免鱼血清中凝集抗体效价和溶菌酶活性均值分别为1:18.89和156.36U/dml,相应的对照组则为1:0.83和138.68dU/ml;受免鱼血中白细胞吞噬百分比和吞噬指数分别为63.38%和6.02,而对照组的分别库存47.95%和5.08;免疫组和对照组白细胞的杀菌指数分别为1.59和1.15,受免鱼的免疫保护力达到73.7%。
Chang Mingxian Chen Xiaoxuan Wu Zhixin\ Hu Xianqin
2001, 20(3).
Abstract:在饲料中添加1‰的虫草多糖,以口服形式对日本沼虾进行免疫,通过连续测定日本沼虾血淋巴吞噬活性,血清中抗菌活力、溶菌活力以及酚氧化酶活力,研究虫草多糖对日本沼虾免疫机能的作用,结果表明:除个别情况外,虫草组的吞噬百分比和吞噬指数、抗菌活力、溶菌活力以及酚氧化酶活力、均高于对照组,因此,投喂虫草多糖能够明显地增强日本沼虾的免疫防御能力。
2001, 20(3).
Abstract:T. thiebautii is transplanted from Sanliku in Japan and cultivated in the house. In its logarithmic phase, TLC, GLC and HPLC analyses are carried out on its total fatty substance, fatty acid, mobile aminoacid, exocellular polysaccharose and its componen
Liu Jun ) Xie Conghua ) Yu Zhanshen ) Liu Yong )
2001, 20(3).
Abstract:Segments from stem root, stem tuber and seed of A. albus and A. konjac were cultured on MS medium supplemented with various combinations of BA, NAA and KT in different concentrations. The results showed that BA and NAA favored the development of
2001, 20(3).
Abstract:钙 (Ca)对果实发育具有极其重要的作用 ,尤其是在与果实质地有关的性状中 ,Ca的含量多少及动态更是解明其机理的基础。虽然有一些报道研究了果实发育过程中Ca的含量变化动态[1 .2 ] ,但不同品种之间的对比研究还很少。而另一方面 ,随着钙调素 (CaM)的发现和深入研究 ,Ca2 +不仅是一种必需的大量营养元素 ,Ca2 +与CaM的结合更重要的是作为偶联胞外信号与胞内生理生化反应的第二信使 ,成为植物代谢和发育的调控者[3,4] 。为此 ,有关Ca2 +与CaM的研究就成为当今植物生理学研究最活跃的领域之一。果树生理研究中虽然有一些…
Ni Dejiang Chen Yuqiong Zhou Jirong Yuan Fangting
2001, 20(3).
Abstract:以卷曲形名优茶为试材,研究了30型滚筒连续杀青机用于名优绿茶增香的效果。结果表明,30型滚筒连续杀青机可用于名优绿茶增香;增香的温度和时间对名优绿茶的品质有较大影响,在温度低于130℃范围,随着增香温度的升高,茶多酚、叶绿素、可溶性糖含量呈下降趋势,氨基酸呈增加趋势;随着时间的增加,茶多酚、叶绿素,氨基酸含量下降,可溶性糖含量增加;在温度超过130℃时,茶叶色泽易黄变,香味易焦变,香气分析结果表明,在增香过程中,二氢海葵内酯、香叶醇、芳樟醇等具有良好香型的香气成分含量明显上升,结合内质分析和感官审评结果,采用30型滚筒连续杀青机增香的最佳温度和时间是120℃10-30s,其次是110℃10-30s,所制茶叶栗香高长。
2001, 20(3).
Abstract:Remote structure analysis system based on internet use Windows DNA framework idea,distributing component module and Winsock technology, implement remote structure analysis function. The system can run steadily and has high efficiency, which can provide ch
Tel: 027-87287364
Postcode: 430070
E-mail:hnlkxb@mail.hzau.edu.cn
Address: No.1 Shizishan Street,Hongshan District,Wuhan, Hubei,China
Jorunal of Huazhong Agricultural University ® 2024 All Rights Reserved Supported by:Beijing E-Tiller Technology Development Co., Ltd.