Tobacco is an important economic crop. Tobacco mosaic virus (TMV) seriously affects the quality and yield of tobacco leaves. Spraying immune inducers is currently a new approach for preventing and controlling plant viral diseases. Treat tobacco with dipotassium glycyrrhizinate, diamine glycyrrhizinate, Gancao cream, sodium cholate, and aluminum reagent respectively; Administer TMV 24 hours later; Quantitative PCR and Western-blot detection will be performed 7 days later. The results showed that treating tobacco with 10 mg/mL sodium cholate could effectively inhibit the expression of TMV genes and the accumulation of CP. The optimal induction time is 24 hours. The induced resistance can last for 10 days. Moreover, sodium cholate treatment can promote the growth of tobacco seedlings. By analyzing the expression of genes related to the JA pathway, SA pathway, and ET pathway, it is inferred that sodium cholate induced TMV resistance is closely related to the JA signaling pathway.