Clarifying the types of viruses in different edible mushrooms during production is the foundation for preventing and controlling fungal virus disease in edible mushrooms. High throughput sequencing technology was used to detect fungal viruses in 53 strains of Pleurotus spp., 10 strains of Ganoderma spp., 17 strains of Flammulina filiformis, and 12 strains of Coprinus comatus. PCR amplification and sequence analysis were performed on newly discovered potential new viruses, and phylogenetic tree analyses were conducted based on sequence comparison. BLASTx sequence alignment results showed that a total of 51 contigs were annotated as 41 viruses detected from 92 strains of four types of edible mushrooms. A total of 13 possible novel viruses were identified through RT-PCR amplification and sequence analysis, including 7 species found in Pleurotus spp., 3 species in Coprinus comatus, 1 species in Flammulina filiformis, and 2 species in Ganoderma spp.. These viruses belong to the +ssRNA viruses, -ssRNA viruses, and dsRNA viruses, and are distributed in 8 viral families, including Barnaviridae, Botuoviridae, Deltaflexiviridae, Mymonaviridae, Partiviridae, Phenuiviridae、Phlegiviridae, and an unclassified member of the order Bunyavirales. In conclusion, there are relatively abundant fungal viruses in the four types of edible mushrooms, among which fungal virus was first discovered in Coprinus comatus and Ganoderma spp.. The discovery of these novel viruses provides a certain reference for further research, prevention, and control of edible mushroom viral diseases.