Soil acidification is currently increasing, but how does soil acidification affect nutrients distribution and accumulation in plant is still not clear. Effects of soil acidification or pH on soil nutrient availability, nutrients concentration and accumulation, migration and distribution in plant were measured, with aim to utilize acidic soil and its nutrients efficiently. Pot experiment was conducted with acidic red soil (pH 5.05) and Cherry tomatoes (Lycopersivon esculentum Mill) as test materials, the soil pH was adjusted with citric acid-sodium hydrogen phosphate buffer and sodium carbonate-sodium bicarbonate buffer to adjust soil pH to 4.0,5.0,6.0 and 7.0. There were 4 treatments in total, with 5 replicates for each treatment. The result shows that measured soil pH were 4.35, 4.92, 5.89, 6.85, respectively, adjusted down by 0.70, 0.13 and increased by 0.84 and 1.80 pH. Soil acidification significantly reduced the leaf area and biomass of Cherry tomato, especially the root biomass,and root to shoot ratio. Soil acidification severely inhibited the accumulation of N, P, K, Mg and Fe in roots, promoted the accumulation and distribution of Fe and Mn in stem, and Ca and Mg in leaves. Significantly decreased N, P, K, Ca, Mg and Fe accumulations and its proportion to total in root, with more N, K, Fe and Mn accumulated in stems, more P, Ca and Mg accumulated in leaves, particularly raised all whole plant Mn concentrations significantly. It concluded that the root was the most severe and sensitive part of whole tomato plant inhibited by soil acidification, as a result, most nutrients were absorbed by the roots, migration were blocked, and accumulation was significantly reduced.