The purpose of this study was to explore the effect of PGF2α signal on the oocyte and early embryo in buffalo, the expression patterns of PTGFR in buffalo oocytes and early embryos development were determined by immunofluorescence and fluorescence quantification. Subsequently, the effects of AL-8810 on the maturation efficiency of oocytes and the efficiency of embryonic development in buffalo were studied by adding different concentrations of AL-8810 into the oocyte maturation medium and embryonic development medium. The results showed that PTGFR protein was widely distributed in buffalo oocytes and embryos, and the expression level of PTGFR gene was significantly higher in 4-16 cell stage than in unfertilized oocytes and morulae (P＜0.01). AL-8810 had no significant differences effect on the nuclear maturation efficiency of buffalo oocytes (P＞0.05), however, high concentration treatment group(1 600 nmol/L) significantly reduced the early apoptosis rate of buffalo oocytes (P＜0.05). AL-8810 at the concentration of 100 and 800 nmol/L significantly improved embryonic development efficiency in 16 cell stage, and the morula formation rate was the highest in the 800 nmol/L group. The above results indicated that inhibition of prostaglandin PGF2α signal effectively improved in vitro maturation quality of oocytes and early embryos development potential in buffalo.