To select optimal reference genes to improve the accuracy of qRT-PCR analysis results in the freshwater snail Bellamya purificata，ten commonly used reference genes，including actβ、18S、gapdh、ef1α、ef1β、rps3、rpl4、tubα、tubβ and sdha were chosen as candidate genes，and geNorm，NormFinder，BestKeeper，ΔCt and RefFinder were used to assess their expression stability during the embryonic and larval stages，as well as across different tissues and between sexes in adult individuals.The results showed that，the expression stability of the candidate genes during the embryonic and larval stages was ranked as follows：gapdh>tubα>18S>ef1α>ef1β>rps3>actβ>tubβ>sdha>rpl4.In terms of inter-tissue expression stability between female and male individuals，the expression stability of the candidate genes were ranked as ef1β>tubα>rps3>ef1α>18S>actβ>gapdh>sdha>tubβ>rpl4 for females，and ef1α>rps3>ef1β>rpl4>tubα>actβ>18S>gapdh>tubβ>sdha for males，respectively.In terms of tissue expression stability between sexes，the ranking was as follows：ef1β>ef1α>rps3>tubα>18S>actβ>gapdh>rpl4>sdha>tubβ.Consequently，gapdh and tubα are recommended as reference genes for qRT-PCR analysis during the embryonic and larval stages，ef1β and tubα are suggested as reference genes for qRT-PCR analysis across female snail tissues，ef1α and rps3 are suggested as reference genes for qRT-PCR analysis across male snail tissues，while ef1α and ef1β are the most appropriate for qRT-PCR analysis between sexes in B.purificata.