To investigate the effects of tea polyphenols on deamination and glycolipid metabolism in Chinese perch，healthy and well-trained fish with an initial body weight of （35.40±1.90） g were intraperitoneally injected with different doses of tea polyphenol solution （0，25，50，and 100 mg/kg），designated as TP0 （blank control group），TP25，TP50，and TP100 groups，respectively. Metabolism-related indices in tissues and serum were measured at 6 h，12 h，24 h，and 48 h after injection. The results showed that，compared to the control group，serum levels of AST，ALT，and TP were significantly reduced in the tea polyphenol-injected groups，and the expression of the liver gdh gene was significantly downregulated after 48 h. Regarding glycolipid metabolism，blood glucose and liver glycogen levels decreased in all tea polyphenol-injected groups，while muscle glycogen content increased. The expression of key liver glycolysis enzyme genes （gk，pk） was upregulated，whereas the expression of the gluconeogenesis-limiting enzyme gene （pepck） was downregulated. Additionally，the expression of fat synthesis genes （accα，fas，srebp1） was significantly reduced，while the expression of the fat transporter gene （cpt1） was significantly increased in all tea polyphenol-injected groups. Among these，the TP25 group exhibited significant reductions in serum levels of high-density lipoprotein （HDL），low-density lipoprotein （LDL），total cholesterol （TCHO），and triglyceride （TG）. Furthermore，the expression of key genes in the AMPK signaling pathway （lkb1，eef2） was significantly upregulated in all tea polyphenol-injected groups. In summary，the tea polyphenol injection group directly inhibited the expression of the gdh gene，thereby reducing amino acid deamination. It also decreased the demand for protein catabolism by activating glycolysis and lipolysis to supply energy，which alleviated the accumulation of liver lipids. Additionally，it inhibited gluconeogenesis through the AMPK signaling pathway，reducing amino acid consumption. The results of this study indicate that tea polyphenols regulate low ammonia emissions through multiple pathways，providing a theoretical basis for the development of efficient and healthy breeding practice in Chinese perch.