Determination of Prochloraz and Imazalil on postharvest sorting line of citrus
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1.College of Plant Science & Technology/Hubei Province Key Laboratory of Plant Pathology, Huazhong Agricultural University,Wuhan 430070, China;2.National R & D Center for Citrus Postharvest Technology,Wuhan 430070, China;3.Yichang Yiling District Agricultural Technology Service Center, Yichang 443100, China

Clc Number:

S663.1

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    Abstract:

    A method for simultaneously detecting the concentrations of Prochloraz and Imazalil at relatively high concentrations was established with high performance liquid chromatography (HPLC) to scientifically standardize the use of Prochloraz and Imazalil in the preservation of citrus fruits.The results showed that the linear relationship between Prochloraz and Imazalil was good within the range of 5-300 μg/mL, indicating that this method has high sensitivity and accuracy.Prochloraz was stable at room temperature and Imazalil had a high rate of degradation in the post-harvest sorting line of Satsuma mandarins.The concentration of Prochloraz and Imazalil after 6 hours of processing 15 tons of Satsuma mandarins decreased by 98.6 μg/mL and 290.3 μg/mL, respectively.It is indicated that this method can be applied to detect the concentration of Prochloraz and Imazalil during the post-harvest processing of citrus fruits, which is beneficial for improving the efficiency of using fungicide and reducing costs of production.

    Fig.1 Chromatogram of Prochloraz and Imazalil
    Fig.2 Stability of Prochloraz and Imazalil in aqueous solution under room temperature
    Fig.3 Stability of Prochloraz and Imazalil in aqueous solution under -20 ℃
    Table 1 Detection of Prochloraz and Imazalil
    Table 2 The precision of the instrument
    Table 3 The recovery of Prochloraz and Imazalil
    Table 4 Changes of mass concentration of Prochloraz and Imazalil
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张家宁,龙复涵,杨中华,李亚荣,谢合平,林杨,郑文艳,于晓,付艳苹. Determination of Prochloraz and Imazalil on postharvest sorting line of citrus[J]. Jorunal of Huazhong Agricultural University,2025,44(2):221-227.

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History
  • Received:October 28,2024
  • Online: April 02,2025
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