Home > Archive>Volume 44, Issue 2, 2025 >158-169. DOI:10.13300/j.cnki.hnlkxb.2025.02.016
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Mechanism of ZmDIR11 regulating resistance to salt stress in maize
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Affiliation:

1.College of Fisheries and Life Sciences， Shanghai University of Ocean， Shanghai 201306， China;2.Institute of Crop Breeding and Cultivation， Shanghai Academy of Agricultural Sciences/ CIMMYT-China Special Maize Research Center/Shanghai Engineering Research Center of Specialty Maize/Shanghai Key Laboratory of Agricultural Genetics and Breeding， Shanghai 201403， China

Clc Number:

S513.3

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    Abstract:

    The ZmDIR11 gene， a member of the DIR gene family， was cloned from maize and its sequence of gene and structures of protein were predicted to mine key genes involved in regulating the resistance to salt stress in maize. The patterns of gene expression were analyzed to further investigate the regulatory mode of ZmDIR11 in salt stress tolerance in maize B73-EMS mutants. The results showed that ZmDIR11 encoded a peptide containing the Dirigent domain， and its promoter region contained structural elements including ARE， MYB， MYC， STRE， LTR， and MBS， all associated with the regulation of abiotic stress. ZmDIR11 was predominantly expressed in maize roots and upregulated under salt stress， with subcellular localization in the endoplasmic reticulum. It was found that the growth and material accumulation of mutant maize plants after salt stress treatment were significantly lower than those of wild-type B73， and the regulation pathway of oxidative metabolism， the accumulation of proline， the synthesis of chlorophyll， the accumulation of lignin， and the transport pathway of Na+/K+ were inhibited. Relevant metabolic indexes of mutant maize plants were significantly lower than those of wild-type B73. It is indicated that ZmDIR11 positively regulates the metabolic pathway of salt tolerance in maize， and its mutation reduces the salt tolerance in maize.

    Fig.1 Basic information about ZmDIR11
    Fig.2 Expression pattern of ZmDIR11 in maize
    Fig.3 The salt tolerance of maize between CK and salt stress treatments
    Fig.4 The antioxidant capacity of maize plants between CK and salt stress treatments
    Fig.5 The accumulation of resistant metabolites between CK and salt stress treatments
    Fig.6 Related indicators of Na+ and K+ ion transport in maize between CK and alt stress treatments
    Table 1 RT-qRCR primer
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赵志雄,关媛,王慧,顾炜,于典司,郑洪建,秦涛. Mechanism of ZmDIR11 regulating resistance to salt stress in maize[J]. Jorunal of Huazhong Agricultural University,2025,44(2):158-169.

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History
  • Received:December 17,2024
  • Online: April 02,2025
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