Fresh leaves of Cerasus humilis ‘Jinou 3’ were used to study the regulatory strategies and underlying mechanisms of the accumulation of γ-aminobutyric acid （GABA） in leaves of C. humilis under abiotic stress. Using soaking， low temperature， and vacuum anaerobic treatment， The accumulation of GABA， the activity of the key enzymes for GABA， and the related physiological and biochemical indexes in leaves under the treatments including soaking， low temperature， and vacuum anaerobic method were measured. The results showed that soaking with monosodium glutamate （MSG）， pyridoxal phosphate （PLP） and NaCl， and vacuum anaerobic method had significant effects on the accumulation of GABA， the activity of the key enzymes for GABA， and the related physiological and biochemical indexes in leaves of C. humilis. The GABA accumulation effect of soaking with MSG for 9 h on the accumulation of GABA in leaves of C. humilis was the best， with 2.3 times of that of untreated leaves （CK）. The content of GABA in leaves of C. humilis after vacuum for 15 h and -20 ℃ for 6 h was 1.99 and 1.97 times higher than that of untreated leaves （CK）. The activity of glutamic acid decarboxylase （GAD）， diamine oxidase （DAO）， polyamine oxidase （PAO）， SOD and POD， and the content of Glu， Ca²⁺， flavonoids， MDA， proline and soluble protein all significantly increased， while the activity of GABA-T significantly decreased. It is indicated that abiotic stress enhances the content of proline as osmotic regulator and the activity of antioxidant enzymes， accumulates the content of Ca²⁺， increases the activity of GAD， catalyzes the synthesis of GABA by Glu， reduces the activity of GABA-T， and decreases GABA degradation in leaves of C. humilis to achieve the enrichment of GABA.