The compositions of HMW-GS in 121 wheat landraces in China were identified and analyzed with SDS-PAGE to mine high-molecular-weight glutenin subunits （HMW-GS） combinations with application value and screen wheat landraces with high-quality. The results showed that 3， 9 and 6 types of allelic variations were detected in the materials tested at the Glu-A1， Glu-B1 and Glu-D1 locus， respectively. Among them， null， 5+10， and 2+12 had the highest frequency at the Glu-A1， Glu-B1 and Glu-D1 locus， with the frequency of 78.51%， 76.86%， and 76.03%， respectively. There were 30 combinations of subunit formed by different types of allelic variation， among which the combination type of null/7+8/2+12 subunit had the highest proportion， accounting for 55.37%. However， the quality score of this combination type of subunits was relatively low， with 6 points only. The combination types of subunits including 1/7+8/5+10， 1/7+9/5+12， 1/7+9/5+10， 2 */7+8/5+10， and 2 */7+9/5+12 had the highest score and became high-quality combinations of subunits. Wheat landraces with these high-quality combinations of subunits including Jiangmai， Qianjiaomai and Bianguangtoukemai， etc. ，can be used as elite genetic resources for improving the quality of modern wheat.