An orthogonal experiment with 4 factors and 3 levels was designed using models of operating including XP2020 and T16， types of pesticide including chlorfenapyr suspension， methomyl indoxacarb water emulsion， and cyanogen caulis EC and silicone adjuvant， volumes of application including 15 L/hm²， 22.5 L/hm²， and 30 L/hm²， and empty column as control to study the optimal operation mode of using agricultural unmanned aerial vehicles （UAVs） to prevent and control the corn borer. 9 collection areas of UAV droplet and investigation areas of prevention and control were set up in the field experiment， and high-gap operation areas of sprayer and blank area were set up as controls to comprehensively evaluate the deposition of droplet and the effect of prevention and control of corn borer under different modes of operation. The significance of each factor was analyzed in depth with Analysis of Variance （ANOVA）. The results showed that there was a positive correlation between the diameter of droplet volume in the experimental area and the amount of deposition under the current experimental conditions （R²=0.712， P<0.05）. The type of pesticide had a significant impact on the effect of prevention and control of corn borer （P<0.05）. The model of operating and the volume of pesticide applied within the range （15~30 L/ hm²） had no significant impact on the effect of prevention and control of corn borer. It is recommended to use T16 UAV in combination with cyanogen caulis EC and silicone adjuvant， with an application rate of 22.5 L/hm² to prevent and control the corn borers taking into account various factors including the effect of prevention and control， the efficiency of operation， economic benefits， and the mitigation of risk. The effect of prevention and control on corn borer can reach 72% after 7 days of application. It will provide a reference for using agricultural UAVs to prevent and control the corn borer larvae and an important guidance for optimizing the application of pesticide and improving the utilization of pesticide.