Home > Archive>Volume 43, Issue 6, 2024 >261-269. DOI:10.13300/j.cnki.hnlkxb.2024.06.025
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Correlation analysis of lutein content and color coefficient in petals of marigold （Tagetes erecta）
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1.College of Horticulture and Forestry Sciences/National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops，Huazhong Agricultural University，Wuhan 430070，China;2.Yunnan Lidar Biological Technology Co.， Ltd.，Wenshan663100，China;3.Guangzhou Lidar Biotechnology Co.， Ltd.，Guangzhou 510663，China;4.Beijing Institute of Landscape Architecture，Beijing 100102，China

Clc Number:

S682.1+1

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    Abstract:

    The colorimeter was used to measure the values of L* （brightness），a* （redness-greenness） and b* （yellowness-blueness） in petals of marigold （Tagetes erecta） to establish a rapid method for determining the lutein content in petals of marigold.The color coefficients including a*/b*，（a*/b*）2，chroma value，hue value and shade value were calculated.UV-spectrophotometry combined with HPLC method was used to determine the lutein content in petals.The correlation between the lutein content and the color coefficients was analyzed.The regression equation was established and confirmed.The results showed that the lutein content in petals of marigold was significantly correlated with a*、a*/b*、（a*/b*）2，hue value and shade value.The regression equation between the lutein content and a* value constructed by cubic function model had the highest degree of fitness，and there was no significant difference between the predicted value and the measured value of the lutein content.It is indicated that the cubic function model based on a* value can quickly and non-destructively predict the content of lutein petals of marigold.

    Fig.1 Flower colors of different marigold genotypes
    Fig.2 Lutein content of petals of different marigold genotypes
    Fig.3 Comparison of the predicted lutein content of the four regression models with the measured values of the four marigold genotypes
    Table 1 Basic information about different marigold genotypes
    Table 2 Color coefficient of petals from different marigold genotypes
    Table 3 Correlation between lutein content and color coefficient of marigold petals
    Table 4 Regression equations of color coefficient and lutein content of marigold petals
    Table 5 Paired t-test results between the measured and predicted values of lutein content
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吕桂芳,臧运平,彭逸,陶正国,炎天埸,张华丽,刘建,何燕红. Correlation analysis of lutein content and color coefficient in petals of marigold （Tagetes erecta）[J]. Jorunal of Huazhong Agricultural University,2024,43(6):261-269.

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  • Received:March 13,2024
  • Online: January 07,2025
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