Home > Archive>Volume 43, Issue 6, 2024 >240-252. DOI:10.13300/j.cnki.hnlkxb.2024.06.023
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Study on differences in response to phytoplasma infection in different resistant jujube germplasms
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Affiliation:

1.College of Biological Sciences and Technology，Beijing Forestry University， Beijing 100083， China;2.Shuangjing Forestry Farm，Aohan Banner，Inner Mongolia Autonomous Region，Chifeng 024300，China;3.National Foundation for Improved Cultivars of Chinese Jujube， Cangzhou 061000， China;4.Shandong Institute of Pomology，Taian271000，China

Clc Number:

S436.65

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    Abstract:

    Jujube witches’ broom disease is a significant threat to jujube trees.To reveal the key biological pathways associated with disease resistance in jujube germplasm in response to phytoplasma infection， a graft-inoculation method was used to evaluate the resistance of 324 jujube germplasm resources to this disease.The study utilized disease-resistant germplasms ‘QS10’，‘UU12’，‘EQ15’ and the susceptible germplasms ‘Zhongyangmuzao’，‘Hupingzao’，‘Luzhao 5’ as materials.The phytoplasma content in leaf tissues was measured at 90 days and 120 days post-infection，and transcriptome sequencing was conducted to elucidate the differential responses.The results showed that the concentration of phytoplasma in resistant germplasm was low and gradually decreased after grafting，while the concentration of phytoplasma in susceptible germplasm was higher and gradually increased over time.The DEGs of both resistant and susceptible germplasm were significantly enriched in pathways such as plant-pathogen interaction，plant hormone signal transduction，and biosynthesis of secondary metabolites.Notable differences were observed in the expression of genes related to peroxisome，photosynthetic pathway and flavonoid biosynthesis pathway.WGCNA analysis revealed that core genes such as lipid phosphatase，RNA editing factor MORF 3，LysM domain receptor-like kinase，and serine/threonine protein kinase sty13-like were associated with resistant germplasm.Research indicated that in response to phytoplasma invasion，the differential expression of genes involved in plant-pathogen interaction，synthesis of secondary metabolites，plant hormone signal transduction，and MAPK signaling pathways may contribute to the differential phenotypes observed between resistant and susceptible germplasms.The peroxisome-related genes are upregulated in disease-resistant germplasm，while photosynthetic genes are less affected by phytoplasma invasion，which may contribute to the normal progression of energy metabolism and redox balance of resistant germplasm，thereby enhancing its disease resistance.Additionally，secondary metabolites such as flavonoids and terpenes may play a role in eliminating the damage caused by active oxygen.

    Fig.1 The phenotype of resistant and susceptible germplasm at 120 days after grafting
    Fig.2 Statistics of differentially expressed genes between resistant and susceptible groups
    Fig.3 GO enrichment results of differentially expressed genes
    Fig.4 KEGG enrichment results of differentially expressed genes obtained by DESeq2 respectively
    Fig.5 Plant and pathogen interaction pathway in jujube tree
    Fig.6 Differential gene analysis of pathways related to phytoplasma infection in jujube trees
    Fig.7 DEG analysis of peroxisome， photosynthetic and flavonoid biosynthesis pathways in response to phytoplasma infection in resistant and susceptible cultivars of jujube
    Fig.8 Gene co-expression network and its core genes
    Table 1 Phytoplasma contents of the germplasm after grafting invasion
    Table 2 Functional annotations of hub genes in different modules
    Reference
    [1] 高京草．中国枣资源研究与利用[D].杨凌:西北农林科技大学,2005.GAO J C.Research and utilization of jujube resources in China[D].Yangling:Northwest A & F University,2005 (in Chinese with English abstract).
    [2] 王海妮,吴云锋,安凤秋,等.枣疯病和酸枣丛枝病植原体16S rDNA和tuf基因的序列同源性分析[J].中国农业科学,2007,40(10):2200-2205.WANG H N,WU Y F,AN F Q,et al.The homology analysis for sequences of 16S rDNA and tuf gene of Phytoplasma from jujube witches’ broom and wild jujube witches’ broom[J].Scientia agricultura sinica,2007,40(10):2200-2205 (in Chinese with English abstract).
    [3] 郭建民,杨俊强,薛新平,等.枣疯病研究进展[J].山西农业科学,2017,45(8):1389-1392.GUO J M, YANG J Q, XUE X P, et al.Research progress on jujube witches’ broom disease[J].Journal of Shanxi Agricultural Sciences,2017,45(8):1389-1392 (in Chinese with English abstract).
    [4] 温秀军,郭晓军,田国忠,等.几个枣树品种和婆枣单株对枣疯病抗性的鉴定[J].林业科学,2005,41(3):88-96.WEN X J, GUO X J, TIAN G Z, et al.Identification of resistances of several jujube cultivars and selected pozao single trees against jujube witches-broom disease[J].Scientia silvae sinicae,2005,41(3):88-96 (in Chinese with English abstract).
    [5] 王合,任争光,潘彦平,等.北京市区古枣树单株种源抗枣疯病测定与抗病品种(系)筛选[J].林业科学,2018,54(8):124-132.WANG H,REN Z G,PAN Y P,et al.Determination of individual jujube trees against jujube witches’-broom disease and screening of resistant varieties from the ancient individual jujube trees growing in Beijing[J].Scientia silvae sinicae,2018,54(8):124-132 (in Chinese with English abstract).
    [6] 刘孟军,周俊义,赵锦,等.极抗枣疯病枣新品种‘星光’[J].园艺学报,2006,33(3):687.LIU M J,ZHOU J Y,ZHAO J,et al.An excellent new cultivar of Chinese jujube with high resistance to jujube witches broom disease ‘Xingguang’[J].Acta horticulturae sinica,2006,33(3):687 (in Chinese with English abstract) .
    [7] WANG L X,LIU S Y,GAO M J,et al.The crosstalk of the salicylic acid and jasmonic acid signaling pathways contributed to different resistance to Phytoplasma infection between the two genotypes in Chinese jujube[J/OL].Frontiers in microbiology,2022,13:800762[2024-03-28].https://doi.org/10.3389/fmicb.2022.800762.
    [8] 田国忠,李志清,赵俊芳,等. 我国部分枣树品种(系)对枣疯病的抗性研究: 第三届中国森林保护学术大会论文摘要集[C].北京:中国林学会, 2010: 25.TIAN G Z, LI Z Q, ZHAO J F, et al. Some Chinese jujube varieties (strains) on the resistance of JWB:Third China Forest Protection Academic Conference Abstract Book[C].Beijing: Chinese Academy of Forestry ,2010: 25(in Chinese).
    [9] LIU Z G,WANG L X,XUE C L,et al.Genome-wide identification of MAPKKK genes and their responses to phytoplasma infection in Chinese jujube (Ziziphus jujuba Mill.)[J/OL].BMC genomics,2020,21(1):142[2024-03-28]. https://doi.org/10.1186/s12864-020-6548-6.
    [10] 李继东,倪静,叶霞,等.枣AP2/ERF转录因子鉴定及其响应枣疯病植原体表达分析[J].园艺学报,2020,47(8):1463-1474.LI J D,NI J,YE X,et al.Genomic identification of jujube AP2/ERF transcription factors and their expression pattern during jujube witches' broom pathogenesis process[J].Acta horticulturae sinica,2020,47(8):1463-1474 (in Chinese with English abstract).
    [11] YE X,WANG H Y,CHEN P,et al.Combination of iTRAQ proteomics and RNA-seq transcriptomics reveals multiple levels of regulation in phytoplasma-infected Ziziphus jujuba Mill[J].Horticulture research,2017,4:17080[2024-03-28].https://doi.org/10.1038/hortres.2017.80.
    [12] 刘伟,霍辰思,王国平,等.枣疯病导致的花异常发育相关基因鉴定及表达分析[J].山西农业大学学报(自然科学版),2021,41(6):57-66.LIU W,HUO C S,WANG G P,et al.Identification and expression analysis of genes related to abnormal flower development induced by jujube madness disease[J].Journal of Shanxi Agricultural University (natural science edition),2021,41(6):57-66 (in Chinese with English abstract).
    [13] 李玲,闫旭宇,张昊,等.基于高通量测序的枣疯病转录组分析[J].分子植物育种,2020,18(11):3537-3543.LI L,YAN X Y,ZHANG H,et al.Transcriptome data analysis of witches’broom disease in Ziziphus jujube based on high-throughput sequencing[J].Molecular plant breeding,2020,18(11):3537-3543 (in Chinese with English abstract).
    [14] 张舒怡,张钟,张春梅,等.基于转录组水平的枣疯病发病机理研究[J].园艺学报,2017,44(7):1287-1298.ZHANG S Y,ZHANG Z,ZHANG C M,et al.Transcriptomic analysis on pathogenesis of witches’ broom disease in Ziziphus jujuba[J].Acta horticulturae sinica,2017,44(7):1287-1298 (in Chinese with English abstract).
    [15] 田国忠,李志清,胡佳续,等.我国部分枣树品种(系)的枣疯病抗性鉴定[J].林业科技开发,2013,27(3):19-25.TIAN G Z,LI Z Q,HU J X,et al.Resistance evaluation of a collection of Chinese jujube cultivars (line) against jujube witches’broom phytoplasma in China[J].China forestry science and technology,2013,27(3):19-25 (in Chinese with English abstract).
    [16] 任争光,王合,林彩丽,等.实时荧光定量PCR(SYBR Green Ⅰ)检测不同抗枣疯病枣树品种嫁接接穗中的植原体浓度[J].植物病理学报,2015,45(5):520-529.REN Z G,WANG H,LIN C L,et al.A real-time (SYBR Green Ⅰ) PCR assay for detection and quantification of jujube witches’-broom phytoplasma in the grafted jujube cultivar scions with different resistance[J].Acta phytopathologica sinica,2015,45(5):520-529 (in Chinese with English abstract).
    [17] HUANG J,ZHANG C M,ZHAO X,et al.The jujube genome provides insights into genome evolution and the domestication of sweetness/acidity taste in fruit trees[J/OL].PLoS genetics,2016,12(12):e1006433[2024-03-28].https://doi.org/10.1371/journal.pgen.1006433.
    [18] XU Y,WANG C,KONG D, et al. Identification of high tolerance to jujube witches’ broom in indian jujube (Ziziphus mauritiana Lam.) and mining differentially expressed genes related to the tolerance through transcriptome analysis[J/OL]. Plants,2023,12(11):2082[2024-03-28]. https://doi.org/10.3390/plants12112082.
    [19] 张毅,吴万亿,刘霞宇,等.利用WGCNA鉴定甘薯耐盐相关共表达网络及核心基因[J].河南农业科学,2021,50(6):16-27.ZHANG Y, WU W Y, LIU X Y, et al.Identification of salt tolerance co-expression modules and hub genes in Ipomoea batatas by WGCNA[J].Journal of Henan agricultural sciences,2021,50(6):16-27 (in Chinese with English abstract).
    [20] KOHL M,WIESE S,WARSCHEID B.Cytoscape:software for visualization and analysis of biological networks[J].Methods in molecular biology,2011,696:291-303.
    [21] 赵锦,刘孟军,周俊义,等.枣疯植原体的分布特点及周年消长规律[J].林业科学,2006,42(8):144-146.ZHAO J, LIU M J, ZHOU J Y, et al.Distribution and YearRound concentration variation of jujube witches’ broom (JWB) Phytoplasma in the plant of Chinese jujube[J].Scientia silvae sinicae,2006,42(8):144-146 (in Chinese with English abstract).
    [22] KARASOV T L,CHAE E,HERMAN J J,et al.Mechanisms to mitigate the trade-off between growth and defense[J].The plant cell,2017,29(4):666-680.
    [23] ZHANG S Y,LI C,SI J P,et al.Action mechanisms of effectors in plant-pathogen interaction[J/OL].International journal of molecular sciences,2022,23(12):6758[2024-03-28].https://doi.org/10.3390/ijms23126758.
    [24] 王会鱼.枣响应植原体侵染及恢复的转录组和蛋白组分析[D].郑州:河南农业大学,2018.WANG H Y.Transcriptome and proteome analysis of jujube in response to phytoplasma infection and recovery[D].Zhengzhou:Henan Agricultural University,2018 (in Chinese with English abstract).
    [25] ZHANG M M, ZHANG S Q.Mitogen-activated protein kinase cascades in plant signaling[J].Journal of integrative plant biology,2022,64(2):301-341.
    [26] RENTEL M C,LECOURIEUX D,OUAKED F,et al.OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis[J].Nature,2004,427(6977):858-861.
    [27] DU M M,ZHAO J H,TZENG D T W,et al.MYC2 orchestrates a hierarchical transcriptional cascade that regulates jasmonate-mediated plant immunity in tomato[J].The plant cell,2017,29(8):1883-1906.
    [28] XUE C L,LIU Z G,WANG L H,et al.The antioxidant defense system in Chinese jujube is triggered to cope with phytoplasma invasion[J].Tree physiology,2020,40(10):1437-1449.
    [29] SEWELAM N,JASPERT N,VAN DER KELEN K,et al.Spatial H2O2 signaling specificity:H2O2 from chloroplasts and peroxisomes modulates the plant transcriptome differentially[J].Molecular plant,2014,7(7):1191-1210.
    [30] 郭艳玲,张鹏英,郭默然,等.次生代谢产物与植物抗病防御反应[J].植物生理学报,2012,48(5):429-434.GUO Y L,ZHANG P Y,GUO M R,et al.Secondary metabolites and plant defence against pathogenic disease[J].China industrial economics,2012,48(5):429-434 (in Chinese with English abstract).
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王超,许亚茹,焦立军,薄文浩,曹明,孔德仓,张琼,庞晓明. Study on differences in response to phytoplasma infection in different resistant jujube germplasms[J]. Jorunal of Huazhong Agricultural University,2024,43(6):240-252.

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  • Received:March 28,2024
  • Online: January 07,2025
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