To study the role of lipin1 （lpin1） gene in the repair of muscle injury in fish， taking Megalobrama amblycephala as the research object， in this study， the temporal and spatial expression of lpin1 gene and its expression change after muscle injury were detected by fluorescent quantitative PCR （qPCR） and whole embryo in situ hybridization （WISH）. The results showed that the ORF sequence of the M. amblycephala lpin1 gene was 2 694 bp， encoding 897 amino acids. Functional domain analysis showed that the Lpin1 protein had highly conserved Lipin-N， Lipin-mid and LNS2 （Lipin/Ned1/Smp2） domains. Phylogenetic analysis revealed that the M. amblycephala Lpin1 was closely related to those of Cyprinus carpio and Danio rerio. The results of qPCR showed that lpin1 was expressed at all stages during the embryonic development of M. amblycephala， and the expression level was the highest in the muscle of adult M. amblycephala. The results of WISH further revealed that lpin1 was specifically expressed in the head and body segments during muscle functioning stage and heartbeating stage. Compared with the uninjured group， the lpin1 expression was the highest at 48 h after mild injury and 216 h after severe injury， respectively， while the pax7 expression was the highest at 144 h after mild injury and 96 h after severe injury， respectively. The above results indicated that lpin1 gene may play an important role in muscle injury and repair of M. amblycephala.