Two-dimensional composite nanosheet materials （GO-MnO₂ NSs） were prepared by combining graphene oxide （GO） with MnO₂ nanosheets （MnO₂ NSs） through π-π stacking force to develop new broad-spectrum nanomaterials for anti-animal borne virus infections. The morphology， structure， crystallinity and element valences of GO-MnO₂ NSs were characterized by TEM， XRD， XPS. The cytotoxicity of GO-MnO₂ NSs was tested by MTT. The results showed that the cell survival rate of 625 μg/mL GO-MnO₂ NSs exceeded 85%. Porcine epidemic diarrhea virus （PEDV） was used as a model virus for coronavirus to study the effect of GO-MnO₂ NSs on PEDV infection through concentration for 50% of maximal effect （EC₅₀）， indirect immunofluorescence and western blot. The results showed that GO-MnO₂ NSs had significant antiviral activity. It is indicated that GO-MnO₂ NSs can eliminate the overexpression of reactive oxygen species caused by viral infection and inhibit cell apoptosis， while upregulating the expression of antiviral factors including interferon and interferon stimulating genes （ISGs） in cells as well， effectively inhibiting viral infection.