Rice is one of the important food crops.Lodging during the production of rice is a common phenomenon that can have a significant impact on the yield and quality of rice.Therefore，developing rice varieties with lodging-resistance is one of the main goals of rice breeding.524 rice germplasm resources were used to further mine the genes that control lodging resistance in rice.Genome-wide association analysis （GWAS） was used to identify the locus qRLG7 that is significantly associated with lodging resistance.The candidate genes regulating lodging resistance in rice were determined by analyzing the expression level and the association of candidate gene.CRISPR-Cas9 technology was used to construct mutant families of two candidate genes on the basis of analyzing the expression characteristics and natural variations of candidate genes in the promoter region.The lodging resistance of mutant families was examined.The results showed that the expression levels of two tandemly arrayed genes （LOC_Os07g37220 and LOC_Os07g37230），both encoding inositol-1-monophosphatase （IMP），were significantly higher than that of the other candidate genes in the internode at 5 cm away from the bottom of culm.The expression levels of the two IMP genes in the highly lodging resistant germplasms were significantly higher than that in the highly lodging-prone germplasms.The SNPs in the promoter regions of the two genes were significantly associated with culm diameter at 5 cm from the bottom （CD5）.The significant SNPs of candidate genes were mainly divided into two haplotypes，and the CD5 values of the two haplotypes were significantly different.The activity of the promoters of two genes had significant differences between the two haplotypes as well.The mutants of the two candidate genes had significantly reduced culm-breaking resistance，culm thickness，culm diameter，plant height，and panicle weight compared to the wild-type rice.It is indicated that the candidate gene LOC_ Os07g37220 and LOC_ Os07g37230 has a certain lodging resistance.It will provide reference for further mining and characterizing genes for the genetic improvement of lodging resistance in rice.