In this study，to investigate the relationship among the three selenoprotein genes selenow2a，selenop2 and selenot2 in yellow catfish，Pelteobagrus fulvidraco，3′/5′ RACE PCR was used to clone the full length cDNAs of three genes，which were 891，1 998 and 1 432 bp with the ORFs of 288，828 and 600 bp，encoding 95，275 and 219 amino acids，respectively. The cDNA sequence analysis of the three genes by online tool SECISerach3 showed that they all contained the stop codons encoding selenocysteine and the selenocysteine insertion sequence （SECIS） element in the 3′ untranslated region （3′ UTR）. Through amino acid sequence comparison and phylogenetic tree analysis，it was found that the predicted amino acid sequences of selenow2a，selenop2 and selenot2 genes shared 82.24%，66.19%，and 79.45% similarity with those of zebrafish （Danio rerio），respectively，and shared 94.74%，68.50% and 90.95% similarity with those of channel catfish （Ictalurus punetaus），respectively，which was shown to be close to the fork of the tree. Quantitative real-time PCR was used to examine the mRNA expression of three selenoprotein genes in the heart，liver，muscle，brain，intestine，spleen，testis and ovaries of yellow catfish，and the results suggested that the mRNA expression levels were tissue-specific. These results suggested that these three genes share the characteristics of selenoprotein family，but are tissue-specific in expression.