The cDNA from cassava Arg7 leaves was used to amplify MePP2CAa gene with RT-PCR to study the role of the protein phosphatase 2C （PP2C） gene in the response of cassava to abiotic stress.The sequence，self-activation activity，and the activity of promoter of the MePP2CAa gene were analyzed bioinformatically.The expression patterns of MePP2CAa gene under different stress and hormone treatments，and its interaction with ABA receptor PYLs were studied.The results showed that the total length of MePP2CAa gene was 1 311 bp，encoding 436 amino acids with the structural domain characteristics of the PP2C family.The protein sequence of MePP2CAa had the highest homology with that of PP2C in Hevea rubber and Jatropha curcas，with 78.95% and 74.09%，respectively，and conserved at the C-terminus.The results of real time fluorescence quantitative PCR showed that the expression level of MePP2CAa gene in cassava storage roots was significantly higher than that in stems and leaves.Mannitol，NaCl，ABA，MeJA，low temperature and SA treatment significantly induced the expression of MePP2CAa gene.The results of analyzing the cis-acting elements of promoters showed that the promoter of MePP2CAa gene included ABRE responsive element，MeJA responsive element，and drought induced element，etc.The results of yeast two hybridization showed that MePP2CAa interacted with MePYL1.It is indicated that the MePP2CAa gene may respond to the abiotic stress in cassava.