Tomatoes were routinely planted in the same field for 3 consecutive years.Using sterile deionized water （CK） as a control，and wood vinegar diluted by 300 times （A），600 times （B），and 900 times （C）.The antibacterial activity of wood vinegar with different dilutions and its effect on the root growth and the structure of bacterial and fungal communities in the rhizosphere of tomato under continuous cropping system were studied to provide theoretical basis and technical support for the sustainable development of the tomato industry using wood vinegar.The results showed that the antibacterial activities of wood vinegars with different dilution significantly inhibited pathogens of bacterial wilt and fusarium wilt.Among the wood vinegars with three dilutions，irrigation with wood vinegar diluted by 600 times was not only beneficial to the growth of the roots，but also helped to improve the diversity of bacteria and fungi in the rhizosphere of tomato under continuous cropping system.Compared with the control （CK），wood vinegars with different dilutions reduced the relative abundance of Actinobacteria to varying degrees，and increased the relative abundance of beneficial microorganisms including Acidobacter，Ascomycota，Neocospora，Aspergillus，Penicillium，Trichoderma，Chaetomium，Trichoderma and Fusarium.It is indicated that wood vinegar has a significant inhibitory effect on the pathogenic microorganisms of tomato bacterial wilt and fusarium wilt.Irrigation with diluted wood vinegar is beneficial to the root growth of tomato and improves the microecology of the rhizosphere of tomato under continuous cropping system.Among them，the improvement effect of wood vinegar diluted by 600 times （B） was the best.