Neuropeptide Y receptors （YR） are thought to mediate multiple physiological functions of teleost NPY family peptides，such as feeding. However，the structure and signaling of fish NPY receptors have not been fully elucidated. In this study，the gene structure，tissue expression and signaling of YR in grass carp （Ctenopharyngodon idellus） were reported. The sequences of seven YR genes of Y1R，Y2R，Y2-2R，Y4R，Y7R，Y8Ra and Y8Rb were obtained in the grass carp genome. Homology analysis of grass carp YR revealed that the amino acid sequences encoded by seven YR isoforms were highly conserved in teleost fish. The results of tissue expression showed that grass carp YR was mainly expressed in central tissues，but also in peripheral tissues，including eyes，gills and intestines. NPY can activate the expression of Y2R，Y4R and Y8Rb in HEK293t cells. Y2R is coupled to the cAMP/PKA signaling pathway and Y4R is coupled to the MAPK/ERK signaling pathway. Y8Rb can activate both cAMP/PKA and MAPK/ERK signaling pathways. Intracerebroventricular injection of NPY could significantly increase the expression of Y1R，Y2R，Y4R and Y8Rb. This study shows that grass carp Y2R，Y4R and Y8Rb are functional and can transmit signals efficiently，and NPY action is likely mediated by these receptors.