In order to explore the optimum spectrum for largemouth bass （Micropterus salmoides） larvae，four treatments including full spectrum （FS），red light （RL），green light （GL） and blue light （BL） were set up under the industrial recirculating aquaculture system. Hoping to find suitable spectral range for largemouth bass breeding，the growth performance，activities of superoxide dismutase （SOD），catalase （CAT），lysozyme （LZM），alkaline phosphatase （AKP），contents of glutathione （GSH） and malondialdehyde （MDA），the relative expression levels of growth hormone （gh），insulin-like growth factors-1 （igf-1），thyroid stimulating hormone （tsh），heat shock protein 70 （hsp70），corticotropin releasing factor （crf），caspase-3 of largemouth bass larvae in different treatments were compared and analyzed. The results showed that the final body length and weight，specific growth rate （SGR），weight gain rate （WGR） and LZM activity of larvae in the full spectrum，green light and blue light groups were significantly higher than those in the red light group （P<0.05），while the contents of MDA，GSH and activities of SOD and CAT were significantly lower than those of the red light group （P<0.05）. The activity of AKP and mRNA expressions of gh，igf-1 and tsh in the full spectrum and green light groups were significantly higher than those in the red light group （P<0.05），and the mRNA expressions of hsp70，crf and caspase-3 were significantly lower than those of the red and blue light groups （P<0.05）. The survival rate （SR） of larvae in the full spectrum group was significantly higher than that in other groups （P<0.05），and showed good cluster phototaxis behavior. In conclusion，full spectrum light source is the most beneficial for largemouth bass larvae cultivation under the industrial recirculating aquaculture.