In order to investigate the regulatory mechanisms of the interferon-induced transmembrane protein 1 gene （Ifitm1） in mammalian follicle development， Ifitm1 was overexpressed and suppressed in mouse granulosa cells （mGCs）. The effects of Ifitm1 on the growth of mGCs were detected by flow cytometry， qRT-PCR and EdU method， the effects of Ifitm1 on the expression of ovulation-related genes in mice were detected by qRT-PCR and Western blot， and the mGCs were treated with related pathway inhibitors to investigate the mechanisms by which Ifitm1 affectes follicle development and ovulation in mice. The results showed that the Ifitm1 gene was successfully overexpressed and suppressed in mGCs. Further studies revealed that interference with Ifitm1 gene expression reduced the expression of cell cycle protein Ccnd1 by 63.5%， decreased the percentage of cells in G0/G1 phase， blocked the cells in G2/M phase， thus inhibiting the proliferation of granulosa cell. Ifitm1 interference also resulted in 1.95-6.76 fold increase in the expression levels of the ovulation marker genes Lhr， Ereg and Cyp19a1 （P<0.05）， and inhibited the expression of p-AKT （Ser473）， a key protein in the PI3K/AKT signalling pathway， blocked the PI3K/AKT signalling pathway， and then inhibited the expression of Ifitm1 gene， but the mRNA levels of Lhr， Ereg and Cyp19a1 remained unchanged in mGCs， suggesting that the Ifitm1 gene affects ovulation through inhibiting the activity of PI3K/AKT signalling pathway. In summary， Ifitm1 plays an important role in mGC growth and follicular ovulation through the PI3K/AKT pathway.