Four fertilization treatments including no fertilization （CK），nitrogen，phosphorus and potassium fertilization （NPK），organic fertilization （OM），and nitrogen，phosphorus and potassium organic fertilization （NPKOM） were applied to the long-term positioning test sites.The denitrification potential of soil under the four fertilization treatments was determined by a combination of field sampling and laboratory analyses.The differences of soil denitrification potential under different fertilization treatments and its intrinsic relationship with environmental factors were compared and analyzed based on the basic physical and chemical properties of soil to study the relationship between the denitrification potential of soil and fertilization treatments.The results showed that the denitrification potential of soil with organic fertilizer was the highest，and that of soil with NPK fertilizer was the lowest.The main factors affecting soil denitrification potential were pH，organic matter，total phosphorus and fast available phosphorus.The application of organic fertilizer increased soil denitrification potential and N₂O emission while increasing soil pH.Nitrogen，phosphorus and potassium fertilizer reduced the soil pH，leading to the reduction of soil denitrification potential.The mixed application of NPK and organic fertilizer can increase soil pH，reduce soil denitrification potential of single application of organic fertilizer，and reduce soil N₂O emissions.