Development and application of synchronous and rapid test strip array device for detecting multiple mycotoxins
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1.Huangpu Customs Technology Center of the People’s Republic of China,Guangzhou 510770,China;2.State Key Laboratory of Food Science and Technology/ College of Food Science and Technology,Jiangnan University,Wuxi 214122,China

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TS207.5

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    Abstract:

    An array device for synchronously and rapidly detecting multiple mycotoxins was developed to further reduce the time of detection and achieve rapid customs clearance of import and export food. Due to the interaction between probe and analyte,it is difficult to quantify the existing scheme of fixing multiple detection lines on a single test paper,and single quality control line cannot guarantee the effectiveness of multiple channels,and the real “synchronous” detection is not achieved. In this article,two kinds of horizontal and vertical arrays adapted to colloidal gold lateral flow immunoassay (LFIA) strips were designed and constructed by 3D printing technology,with four channels synchronous detection capability. The LFIA strip of aflatoxin B1 (AFB1),zearalenone (ZEN),ochratoxin A (OTA) and deoxynienol (DON) was integrated into the array and used to detect corn,corn meal and wheat kernel. Results showed that the detection limit (LOD) of the four toxins was 0.031 ng/mL,0.19 ng/mL,0.78 ng/mL,0.22 ng/mL,respectively,with recoveries ranging from 74.42% to 112.40%. There was no cross-reaction. The synchronous detection of the four indicators of a single sample,including the pretreatment time,was completed within 40 minutes. The array device constructed will provide a potential equipment support for synchronous and rapid detection of multiple mycotoxins.

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徐正华,杨晗婕,吴思超,张毅. Development and application of synchronous and rapid test strip array device for detecting multiple mycotoxins[J]. Jorunal of Huazhong Agricultural University,2022,41(5):283-290.

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History
  • Received:May 09,2022
  • Revised:
  • Adopted:
  • Online: October 11,2022
  • Published: