To explore the effect and mechanism of ginger extract （GE） on high temperature stress，an acute heat stress （37 ℃） model of Caenorhabditis elegans was established.The lifespan and movement status of wild-type nematodes，the lifespan of mutants under acute heat stress and the mRNA expression of genes related to heat shock response （HSR） in wild-type nematodes under different degree of heat stress after GE treatment were measured.The results showed that compared with the control group（CK），30，60 and 120 μg/mL GE treatment significantly prolonged the heat-stress lifespan of wild-type nematodes by 16.96%，18.73% and 14.44%，respectively，and significantly improved the movement status of worms at 1st，2nd，and 3rd hour under heat stress.GE-mediated extension of heat-stress lifespan in wild-type nematodes depended on the transcription factor hsf-1，which regulated the heat shock response.GE intervention significantly up-regulated the mRNA expression of the HSR-related genes，hsf-1，hsp-90，hsp-16.2，and the protein expression of HSP-16.2 in wild-type nematodes at 20 ℃.Besides，GE had a regulatory effect on the expression levels of HSR-related genes in wild-type nematodes with different degrees of heat stress （transient heat stress，continuous heat stress）.Compared with normal temperature （20 ℃），transient heat stress significantly up-regulated the expression of hsf-1，hsp-16.2，hsp-70 and hsp-90 in nematodes，while GE treatment significantly down-regulated the expression of HSR-related genes.Compared with normal temperature （20 ℃），continuous heat-stress down-regulated the expression of HSR-related genes in nematodes，and GE treatment significantly up-regulated the expression of hsp-16.2 and the expression of hsf-1 and hsp-90 showed an up-regulation trend，indicating that GE treatment reversed the effect of heat stress on the mRNA expression of HSR-related genes to a certain extent.According to the results above，ginger extract could alleviate heat stress in C. elegans by regulating HSR.