The Lane Late navel orange with five approach-grafting rootstock combinations including Poncirus trifoliate，red tangerine，Citrus junos，Poncirus trifoliate+red tangerine，and Poncirus trifoliate+Citrus junos in Qingjiang River Basin of Hubei Province was used to study the effects of different rootstock combinations on the nutrition，growth，and fruit quality of Lane Late navel orange.Results showed that the uptake efficiency of iron，copper，and zinc of Poncirus trifoliate+Citrus junos and Citrus junos were higher than that of other rootstock combinations.The same patterns were also observed in the uptake efficiency of calcium and magnesium of red tangerine and Poncirus trifoliate+red tangerine and the uptake efficiency of boron of Poncirus trifoliate.Comparing plant height，crown width and branch length，the growth and tree vigor of different rootstock combinations was in the decreasing order of red tangerine > Poncirus trifoliate+red tangerine > Poncirus trifoliate+Citrus junos > Citrus junos > Poncirus trifoliate.Through the comprehensive comparison of fruit size，peel thickness，soluble solid matter，titratable acid，soluble solids and acid ratio and other indicators，the fruit quality of different rootstock combinations was in the decreasing order of Poncirus trifoliate+red tangerine > Poncirus trifoliate > Poncirus trifoliate+Citrus junos > red tangerine> Citrus junos.The fruit granulation rate and granulation index of navel orange in Poncirus trifoliate+red tangerine and Poncirus trifoliate+Citrus junos were significantly lower than those in other rootstock combinations.On the whole，the nutrient uptake efficiency of navel orange in Poncirus trifoliate+red tangerine and Poncirus trifoliate+Citrus junos are higher，the tree vigor is stronger，and the comprehensive fruit quality is the best，which is suitable for promotion in the Qingjiang River Basin.