Yuhuang pomelo （C.grandis Osbeck）is a local citrus variety grown in Jianshi County， Hubei Province.To provide a scientific basis for the harvest of the fruit and the choice between seeded and seedless in production， the appearance and its internal quality of fruits among various harvest periods of different types were compared and analyzed.The results showed that the peel coloring degree and soluble solid content of fruits harvested 240 days post anthesis was comparatively highest.Meanwhile， the seeded type demonstrated the lowest titratable acid content and the highest solid-acid ratio （by 14.62）， while the seedless fruit on the 222 days post anthesis had the highest solid-acid ratio （by 14.55）.In general， the appearance and flavor quality of seeded fruit harvested 240 days post anthesis was the best， followed by those harvested 222 days post anthesis.The content of Vc （by 35.98 mg/100 g in seeded type， 31.77 mg/100 g in seedless type） and main volatile substance Germacrene D in seeded fruit were significantly higher than those in seedless fruit 240 days post anthesis.It indicates that timely harvesting and pollination can optimize the fruit quality of Yuhuang pomelo， so scientific harvesting and increasing pollination rate can be used to increase orchard revenue.