In the biogas project of large-scale farms，the environmental nitrogen and phosphorus pollution is often caused by the inability of biogas slurry to be consumed nearby，which makes the recovery technology of nitrogen and phosphorus nutrient from biogas slurry more concerned.In this article，the low C/N ratio biogas slurry was enriched and cultured by adding glucose carbon source to realize the value-added utilization of wastewater and recovery of nutrient from biogas slurry.The biogas slurry in pig farms with initial C/N of 1.5 was added with 2 g/L，4 g/L，6 g/L，8 g/L and 10 g/L glucose carbon sources，respectively.The OD₆₀₀ increased first and then decreased after adding carbon sources，and reached the maximum at 2-3 d.The total protein growth rate of 6 g/L was the highest in each group.The results showed that the optimal conditions for enrichment culture of the indigenous microflora in biogas slurry were the addition of 6 g/L glucose and 2 d treatment based on the comprehensive economy.Under the optimal conditions，the removal rate of TN and TP from biogas slurry were 66.1% and 77.5%，respectively.0.72 g/L total protein was produced by enrichment of the indigenous microflora in biogas slurry.The biomass dry weight increased by 146.1%.29.0% of total nitrogen and 59.7% of total phosphorus were recovered.It is indicated that the enrichment culture of the indigenous microflora in biogas slurry can be effectively used for the recovery of nitrogen and phosphorus nutrient.Microbial dry weight and protein growth can be used as a pretreatment method for the advanced treatment of biogas slurry in pig farms.