To investigate the utilization of nitrogen in chicken manure by black soldier fly larvae （BSFL） under treatment with urease inhibitors，three different urease inhibitors，N-butylthiophosphoric triamide （NBPT），acetylhydroxamic acid （AHA） and hydroquninone （HQ） were added to the treatment groups，and chicken manure without urease inhibitors was used as the control group.All groups were inoculated with 800 BSFL，and the larvae growth and transformation efficiency，NH₃ emission，and changes in the physical and chemical properties of the substrate were compared among different groups.The results showed that compared with the control group，urease inhibitors could effectively reduce NH₃ emission and increase fresh larvae weight during bioconversion of chicken manure by BSFL.The BSFL body weight and protein content of NBPT，AHA and HQ groups increased by （64.75±3.46） g，（71.33±3.38） g，and （78.84±1.84） g，respectively，and the BSFL nitrogen content was （14.47±1.14）%，（15.92±0.34）% and （16.24±0.45）%，respectively.The results indicated all three treatments could improve the body weight and protein content of BSFL，especially for the HQ treatment，which could effectively reduce NH₃ emission and significantly increase the fresh BSFL weight.