In order to study the effect of buckwheat honey on improving the liver injury induced by alcohol and the dysbiosis of gut microbiota in mice,the liver index,activity of serum transaminase was measured after buckwheat honey administration in the mice with liver injury induced by alcohol. The effects of the liver index,activity of serum transaminase on the liver lipid metabolism,oxidative stress and liver tissue were analyzed. The changes of gut microbiota in mice were analyzed with highthroughput sequencing. The results showed that compared with the alcohol model group,buckwheat honey significantly reduced the levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),malondialdehyde (MDA),and the liver fat droplet phenomenon caused by alcohol (P<0.05),reduced the level of liver triglyceride (TG),improved the activity of superoxide dismutase (SOD),and significantly improved the steatosis. Compared with the alcohol model group,the intake of buckwheat honey improved the diversity and richness of gut microbiota in mice caused by alcohol intake. At the phylum level,the abundance of Bacteroidetes increased by 7.18%,the abundance of Firmicutes decreased by 15.59% and the ratio of Firmicutes to Bacteroidetes decreased by 21.25% and restored to the level of blank control group. The results of further analyzing the top 36 abundant microorganisms at the genus level showed that buckwheat honey significantly improved the changes of 16 bacterial genera caused by alcohol. It is indicated that buckwheat honey can improve the liver injury and gut microbiota imbalance caused by alcohol in mice,and can be developed and utilized as a raw material for liver protection products.