Expression of an novel 5enolpyruvylshikimate3phosphate synthase genes (EPSPS) in plastids conferring glyphosate resistance in tobacco
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    Abstract:

    A novel glyphosatetolerant EPSPS gene AroAI.va* derived from Isoptericola variabilisn and driven by maizederived composite ciselement was efficiently and simultaneously expressed in the plastids of leaves and nongreen tissues of tobacco. The results of transcription and proteinlevel determination of the homoplastomic transplastomic lines showed that the AroAI.va* gene was effectively expressed in the leaf and root tissues. The T0 seeds of homoplastomic transplastomic plants pLSZ3#2 and pLSZ3#3 tolerated more than 12 mmol/L glyphosate treatment. The transgenic plants cultivated in the greenhouse tolerated the spraying treatment with glyphosate at a dose of 1 800 mg/L. Reciprocal crosses between wildtype and transgenic plants confirmed that the glyphosateresistant transgenic plants obtained by plastid transformation had the characteristics of maternal inheritance.

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李凡,路史展,刘羽鑫,林拥军,吴高兵,周菲. Expression of an novel 5enolpyruvylshikimate3phosphate synthase genes (EPSPS) in plastids conferring glyphosate resistance in tobacco[J]. Jorunal of Huazhong Agricultural University,2021,40(5).

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History
  • Received:May 18,2021
  • Revised:
  • Adopted:
  • Online: September 29,2021
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